Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (<i>Sus scrofa</i>)
In recent years, cases of hepatitis E virus (HEV) infection have increased in Europe in association with the consumption of contaminated food, mainly from pork products but also from wild boars. The animal’s serum is usually tested for the presence of anti-HEV antibodies and viral RNA but,...
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MDPI AG
2020-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/3/450 |
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| author | Alejandro Navarro Carmen Bárcena Pilar Pozo Alberto Díez-Guerrier Irene Martínez Coral Polo Clara Duque David Rodríguez-Lázaro Joaquín Goyache Nerea García |
| author_facet | Alejandro Navarro Carmen Bárcena Pilar Pozo Alberto Díez-Guerrier Irene Martínez Coral Polo Clara Duque David Rodríguez-Lázaro Joaquín Goyache Nerea García |
| author_sort | Alejandro Navarro |
| collection | DOAJ |
| description | In recent years, cases of hepatitis E virus (HEV) infection have increased in Europe in association with the consumption of contaminated food, mainly from pork products but also from wild boars. The animal’s serum is usually tested for the presence of anti-HEV antibodies and viral RNA but, in many cases such as during hunting, an adequate serum sample cannot be obtained. In the present study, liver transudate was evaluated as an alternative matrix to serum for HEV detection. A total of 125 sera and liver transudates were tested by enzyme-linked immunosorbent assay at different dilutions (1:2, 1:10, 1:20), while 58 samples of serum and liver transudate were checked for the presence of HEV RNA by RT-qPCR. Anti- HEV antibodies were detected by ELISA in 68.0% of the serum samples, and in 61.6% of the undiluted transudate, and in 70.4%, 56.8%, and 44.8% of 1:2, 1:10, or 1:20 diluted transudate, respectively. The best results were obtained for the liver transudate at 1:10 dilution, based on the Kappa statistic (0.630) and intraclass correlation coefficient (0.841). HEV RNA was detected by RT-qPCR in 22.4% of the serum samples and 6.9% of the transudate samples, all samples used for RT-qPCR were positive by ELISA. Our results indicate that liver transudate may be an alternative matrix to serum for the detection of anti-HEV antibodies. |
| first_indexed | 2024-04-14T06:25:54Z |
| format | Article |
| id | doaj.art-256d55724c5f4e5ab6f08c0f17241e7a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-14T06:25:54Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-256d55724c5f4e5ab6f08c0f17241e7a2022-12-22T02:07:50ZengMDPI AGMicroorganisms2076-26072020-03-018345010.3390/microorganisms8030450microorganisms8030450Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (<i>Sus scrofa</i>)Alejandro Navarro0Carmen Bárcena1Pilar Pozo2Alberto Díez-Guerrier3Irene Martínez4Coral Polo5Clara Duque6David Rodríguez-Lázaro7Joaquín Goyache8Nerea García9VISAVET Health Surveillance Center, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Center, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Center, Complutense University of Madrid, 28040 Madrid, SpainMAEVA SERVET, S.L., Alameda del Valle, 28749 Madrid, SpainVISAVET Health Surveillance Center, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Center, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Center, Complutense University of Madrid, 28040 Madrid, SpainMicrobiology Division, Faculty of Sciences, Universidad de Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, SpainVISAVET Health Surveillance Center, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Center, Complutense University of Madrid, 28040 Madrid, SpainIn recent years, cases of hepatitis E virus (HEV) infection have increased in Europe in association with the consumption of contaminated food, mainly from pork products but also from wild boars. The animal’s serum is usually tested for the presence of anti-HEV antibodies and viral RNA but, in many cases such as during hunting, an adequate serum sample cannot be obtained. In the present study, liver transudate was evaluated as an alternative matrix to serum for HEV detection. A total of 125 sera and liver transudates were tested by enzyme-linked immunosorbent assay at different dilutions (1:2, 1:10, 1:20), while 58 samples of serum and liver transudate were checked for the presence of HEV RNA by RT-qPCR. Anti- HEV antibodies were detected by ELISA in 68.0% of the serum samples, and in 61.6% of the undiluted transudate, and in 70.4%, 56.8%, and 44.8% of 1:2, 1:10, or 1:20 diluted transudate, respectively. The best results were obtained for the liver transudate at 1:10 dilution, based on the Kappa statistic (0.630) and intraclass correlation coefficient (0.841). HEV RNA was detected by RT-qPCR in 22.4% of the serum samples and 6.9% of the transudate samples, all samples used for RT-qPCR were positive by ELISA. Our results indicate that liver transudate may be an alternative matrix to serum for the detection of anti-HEV antibodies.https://www.mdpi.com/2076-2607/8/3/450hepatitis e viruspigwild boarliver transudatediagnosisserologyelisapcr |
| spellingShingle | Alejandro Navarro Carmen Bárcena Pilar Pozo Alberto Díez-Guerrier Irene Martínez Coral Polo Clara Duque David Rodríguez-Lázaro Joaquín Goyache Nerea García Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (<i>Sus scrofa</i>) Microorganisms hepatitis e virus pig wild boar liver transudate diagnosis serology elisa pcr |
| title | Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (<i>Sus scrofa</i>) |
| title_full | Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (<i>Sus scrofa</i>) |
| title_fullStr | Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (<i>Sus scrofa</i>) |
| title_full_unstemmed | Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (<i>Sus scrofa</i>) |
| title_short | Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (<i>Sus scrofa</i>) |
| title_sort | liver transudate a potential alternative to detect anti hepatitis e virus antibodies in pigs and wild boars i sus scrofa i |
| topic | hepatitis e virus pig wild boar liver transudate diagnosis serology elisa pcr |
| url | https://www.mdpi.com/2076-2607/8/3/450 |
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