Bacterial Endophytes of Spring Wheat Grains and the Potential to Acquire Fe, Cu, and Zn under Their Low Soil Bioavailability
Wheat grains are usually low in essential micronutrients. In resolving the problem of grain micronutritional quality, microbe-based technologies, including bacterial endophytes, seem to be promising. Thus, we aimed to (1) isolate and identify grain endophytic bacteria from selected spring wheat vari...
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MDPI AG
2021-05-01
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| Online Access: | https://www.mdpi.com/2079-7737/10/5/409 |
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| author | Orysia Makar Agnieszka Kuźniar Ostap Patsula Yana Kavulych Volodymyr Kozlovskyy Agnieszka Wolińska Ewa Skórzyńska-Polit Olena Vatamaniuk Olga Terek Nataliya Romanyuk |
| author_facet | Orysia Makar Agnieszka Kuźniar Ostap Patsula Yana Kavulych Volodymyr Kozlovskyy Agnieszka Wolińska Ewa Skórzyńska-Polit Olena Vatamaniuk Olga Terek Nataliya Romanyuk |
| author_sort | Orysia Makar |
| collection | DOAJ |
| description | Wheat grains are usually low in essential micronutrients. In resolving the problem of grain micronutritional quality, microbe-based technologies, including bacterial endophytes, seem to be promising. Thus, we aimed to (1) isolate and identify grain endophytic bacteria from selected spring wheat varieties (bread Oksamyt myronivs’kyi, Struna myronivs’ka, Dubravka, and emmer Holikovs’ka), which were all grown in field conditions with low bioavailability of microelements, and (2) evaluate the relationship between endophytes’ abilities to synthesize auxins and the concentration of Fe, Zn, and Cu in grains. The calculated biological accumulation factor (BAF) allowed for comparing the varietal ability to uptake and transport micronutrients to the grains. For the first time, bacterial endophytes were isolated from grains of emmer wheat <i>T. turgidum</i> subsp. <i>dicoccum</i>. Generally, the 12 different isolates identified in the four varieties belonged to the genera <i>Staphylococcus</i>, <i>Pantoea</i>, <i>Sphingobium</i>, <i>Bacillus</i>, <i>Kosakonia</i>, and <i>Micrococcu</i>s (NCBI accession numbers: MT302194—MT302204, MT312840). All the studied strains were able to synthesize the indole-related compounds (IRCs; max: 16.57 µg∙mL<sup>−1</sup>) detected using the Salkowski reagent. The IRCs produced by the bacterial genera <i>Pantoea</i> spp. and <i>Bacillus</i> spp. isolated from high-yielding Oksamyt myronivs’kyi and Holikovs’ka grains may be considered as one of the determinants of the yield of wheat and its nutritional characteristics. |
| first_indexed | 2024-03-10T11:41:10Z |
| format | Article |
| id | doaj.art-256e97a94a104540b998ba8fd8ecce34 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T11:41:10Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj.art-256e97a94a104540b998ba8fd8ecce342023-11-21T18:27:09ZengMDPI AGBiology2079-77372021-05-0110540910.3390/biology10050409Bacterial Endophytes of Spring Wheat Grains and the Potential to Acquire Fe, Cu, and Zn under Their Low Soil BioavailabilityOrysia Makar0Agnieszka Kuźniar1Ostap Patsula2Yana Kavulych3Volodymyr Kozlovskyy4Agnieszka Wolińska5Ewa Skórzyńska-Polit6Olena Vatamaniuk7Olga Terek8Nataliya Romanyuk9Department of Plant Physiology and Ecology, Ivan Franko National University of Lviv, 4 Hrushevsky Street, 79005 Lviv, UkraineDepartment of Biology and Biotechnology of Microorganisms, The John Paul II Catholic University of Lublin, 1I Konstantynów Street, 20-708 Lublin, PolandDepartment of Plant Physiology and Ecology, Ivan Franko National University of Lviv, 4 Hrushevsky Street, 79005 Lviv, UkraineDepartment of Plant Physiology and Ecology, Ivan Franko National University of Lviv, 4 Hrushevsky Street, 79005 Lviv, UkraineInstitute of Ecology of the Carpathians, NAS of Ukraine, 4 Kozelnytska Street, 79000 Lviv, UkraineDepartment of Biology and Biotechnology of Microorganisms, The John Paul II Catholic University of Lublin, 1I Konstantynów Street, 20-708 Lublin, PolandDepartment of Plant Physiology and Biotechnology, The John Paul II Catholic University of Lublin, 1I Konstantynów Street, 20-708 Lublin, PolandSoil and Crop Sciences Section, School of Integrative Plant Science, Cornell University, 608 Bradfield Hall, Ithaca, NY 14853, USADepartment of Plant Physiology and Ecology, Ivan Franko National University of Lviv, 4 Hrushevsky Street, 79005 Lviv, UkraineDepartment of Plant Physiology and Ecology, Ivan Franko National University of Lviv, 4 Hrushevsky Street, 79005 Lviv, UkraineWheat grains are usually low in essential micronutrients. In resolving the problem of grain micronutritional quality, microbe-based technologies, including bacterial endophytes, seem to be promising. Thus, we aimed to (1) isolate and identify grain endophytic bacteria from selected spring wheat varieties (bread Oksamyt myronivs’kyi, Struna myronivs’ka, Dubravka, and emmer Holikovs’ka), which were all grown in field conditions with low bioavailability of microelements, and (2) evaluate the relationship between endophytes’ abilities to synthesize auxins and the concentration of Fe, Zn, and Cu in grains. The calculated biological accumulation factor (BAF) allowed for comparing the varietal ability to uptake and transport micronutrients to the grains. For the first time, bacterial endophytes were isolated from grains of emmer wheat <i>T. turgidum</i> subsp. <i>dicoccum</i>. Generally, the 12 different isolates identified in the four varieties belonged to the genera <i>Staphylococcus</i>, <i>Pantoea</i>, <i>Sphingobium</i>, <i>Bacillus</i>, <i>Kosakonia</i>, and <i>Micrococcu</i>s (NCBI accession numbers: MT302194—MT302204, MT312840). All the studied strains were able to synthesize the indole-related compounds (IRCs; max: 16.57 µg∙mL<sup>−1</sup>) detected using the Salkowski reagent. The IRCs produced by the bacterial genera <i>Pantoea</i> spp. and <i>Bacillus</i> spp. isolated from high-yielding Oksamyt myronivs’kyi and Holikovs’ka grains may be considered as one of the determinants of the yield of wheat and its nutritional characteristics.https://www.mdpi.com/2079-7737/10/5/409spring wheat<i>T. aestivum</i> L.emmer wheatyieldendophytic bacteriairon |
| spellingShingle | Orysia Makar Agnieszka Kuźniar Ostap Patsula Yana Kavulych Volodymyr Kozlovskyy Agnieszka Wolińska Ewa Skórzyńska-Polit Olena Vatamaniuk Olga Terek Nataliya Romanyuk Bacterial Endophytes of Spring Wheat Grains and the Potential to Acquire Fe, Cu, and Zn under Their Low Soil Bioavailability Biology spring wheat <i>T. aestivum</i> L. emmer wheat yield endophytic bacteria iron |
| title | Bacterial Endophytes of Spring Wheat Grains and the Potential to Acquire Fe, Cu, and Zn under Their Low Soil Bioavailability |
| title_full | Bacterial Endophytes of Spring Wheat Grains and the Potential to Acquire Fe, Cu, and Zn under Their Low Soil Bioavailability |
| title_fullStr | Bacterial Endophytes of Spring Wheat Grains and the Potential to Acquire Fe, Cu, and Zn under Their Low Soil Bioavailability |
| title_full_unstemmed | Bacterial Endophytes of Spring Wheat Grains and the Potential to Acquire Fe, Cu, and Zn under Their Low Soil Bioavailability |
| title_short | Bacterial Endophytes of Spring Wheat Grains and the Potential to Acquire Fe, Cu, and Zn under Their Low Soil Bioavailability |
| title_sort | bacterial endophytes of spring wheat grains and the potential to acquire fe cu and zn under their low soil bioavailability |
| topic | spring wheat <i>T. aestivum</i> L. emmer wheat yield endophytic bacteria iron |
| url | https://www.mdpi.com/2079-7737/10/5/409 |
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