Loaded delta-hemolysin shapes the properties of Staphylococcus aureus membrane vesicles
BackgroundMembrane vesicles (MVs) are nanoscale vesicular structures produced by bacteria during their growth in vitro and in vivo. Some bacterial components can be loaded in bacterial MVs, but the roles of the loaded MV molecules are unclear.MethodsMVs of Staphylococcus aureus RN4220 and its deriva...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-10-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1254367/full |
| _version_ | 1797665185183301632 |
|---|---|
| author | Juan Chen Yuhuan Lv Yuhuan Lv Weilong Shang Yi Yang Yuting Wang Zhen Hu Xiaonan Huang Rong Zhang Jizhen Yuan Jingbin Huang Xiancai Rao |
| author_facet | Juan Chen Yuhuan Lv Yuhuan Lv Weilong Shang Yi Yang Yuting Wang Zhen Hu Xiaonan Huang Rong Zhang Jizhen Yuan Jingbin Huang Xiancai Rao |
| author_sort | Juan Chen |
| collection | DOAJ |
| description | BackgroundMembrane vesicles (MVs) are nanoscale vesicular structures produced by bacteria during their growth in vitro and in vivo. Some bacterial components can be loaded in bacterial MVs, but the roles of the loaded MV molecules are unclear.MethodsMVs of Staphylococcus aureus RN4220 and its derivatives were prepared. Dynamic light scattering analysis was used to evaluate the size distribution, and 4D-label-free liquid chromatography–tandem mass spectrometry analysis was performed to detect protein composition in the MVs. The site-mutation S. aureus RN4220-Δhld and agrA deletion mutant RN4220-ΔagrA were generated via allelic replacement strategies. A hemolysis assay was performed with rabbit red blood cells. CCK-8 and lactate dehydrogenase release assays were used to determine the cytotoxicity of S. aureus MVs against RAW264.7 macrophages. The serum levels of inflammatory factors such as IL-6, IL-1β, and TNFα in mice treated with S. aureus MVs were detected with an enzyme-linked immunosorbent assay kit.ResultsDelta-hemolysin (Hld) was identified as a major loaded factor in S. aureus MVs. Further study showed that Hld could promote the production of staphylococcal MVs with smaller sizes. Loaded Hld affected the diversity of loaded proteins in MVs of S. aureus RN4220. Hld resulted in decreased protein diversity in MVs of S. aureus. Site-mutation (RN4220-Δhld) and agrA deletion (RN4220-ΔagrA) mutants produced MVs (ΔhldMVs and ΔagrAMVs) with a greater number of bacterial proteins than those derived from wild-type RN4220 (wtMVs). Moreover, Hld contributed to the hemolytic activity of wtMVs. Hld-loaded wtMVs were cytotoxic to macrophage RAW264.7 cells and could stimulate the production of inflammatory factor IL-6 in vivo.ConclusionThis study presented that Hld was a major loaded factor in S. aureus MVs, and the loaded Hld played vital roles in the MV-property modification. |
| first_indexed | 2024-03-11T19:41:14Z |
| format | Article |
| id | doaj.art-2573a167b0694e938503b364d36af89a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T19:41:14Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2573a167b0694e938503b364d36af89a2023-10-06T10:00:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-10-011410.3389/fmicb.2023.12543671254367Loaded delta-hemolysin shapes the properties of Staphylococcus aureus membrane vesiclesJuan Chen0Yuhuan Lv1Yuhuan Lv2Weilong Shang3Yi Yang4Yuting Wang5Zhen Hu6Xiaonan Huang7Rong Zhang8Jizhen Yuan9Jingbin Huang10Xiancai Rao11Department of Pharmacy, The Second Affiliated Hospital, Army Medical University, Chongqing, ChinaDepartment of Microbiology, College of Basic Medical Science, Army Medical University, Chongqing, ChinaDepartment of Clinical Laboratory, The 971st Hospital of Chinese People's Liberation Army Navy, Qingdao, ChinaDepartment of Microbiology, College of Basic Medical Science, Army Medical University, Chongqing, ChinaDepartment of Microbiology, College of Basic Medical Science, Army Medical University, Chongqing, ChinaDepartment of Microbiology, College of Basic Medical Science, Army Medical University, Chongqing, ChinaDepartment of Microbiology, College of Basic Medical Science, Army Medical University, Chongqing, ChinaDepartment of Microbiology, College of Basic Medical Science, Army Medical University, Chongqing, ChinaDepartment of Pharmacy, The Second Affiliated Hospital, Army Medical University, Chongqing, ChinaDepartment of Clinical Laboratory, The 971st Hospital of Chinese People's Liberation Army Navy, Qingdao, ChinaDepartment of Pharmacy, The Second Affiliated Hospital, Army Medical University, Chongqing, ChinaDepartment of Microbiology, College of Basic Medical Science, Army Medical University, Chongqing, ChinaBackgroundMembrane vesicles (MVs) are nanoscale vesicular structures produced by bacteria during their growth in vitro and in vivo. Some bacterial components can be loaded in bacterial MVs, but the roles of the loaded MV molecules are unclear.MethodsMVs of Staphylococcus aureus RN4220 and its derivatives were prepared. Dynamic light scattering analysis was used to evaluate the size distribution, and 4D-label-free liquid chromatography–tandem mass spectrometry analysis was performed to detect protein composition in the MVs. The site-mutation S. aureus RN4220-Δhld and agrA deletion mutant RN4220-ΔagrA were generated via allelic replacement strategies. A hemolysis assay was performed with rabbit red blood cells. CCK-8 and lactate dehydrogenase release assays were used to determine the cytotoxicity of S. aureus MVs against RAW264.7 macrophages. The serum levels of inflammatory factors such as IL-6, IL-1β, and TNFα in mice treated with S. aureus MVs were detected with an enzyme-linked immunosorbent assay kit.ResultsDelta-hemolysin (Hld) was identified as a major loaded factor in S. aureus MVs. Further study showed that Hld could promote the production of staphylococcal MVs with smaller sizes. Loaded Hld affected the diversity of loaded proteins in MVs of S. aureus RN4220. Hld resulted in decreased protein diversity in MVs of S. aureus. Site-mutation (RN4220-Δhld) and agrA deletion (RN4220-ΔagrA) mutants produced MVs (ΔhldMVs and ΔagrAMVs) with a greater number of bacterial proteins than those derived from wild-type RN4220 (wtMVs). Moreover, Hld contributed to the hemolytic activity of wtMVs. Hld-loaded wtMVs were cytotoxic to macrophage RAW264.7 cells and could stimulate the production of inflammatory factor IL-6 in vivo.ConclusionThis study presented that Hld was a major loaded factor in S. aureus MVs, and the loaded Hld played vital roles in the MV-property modification.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1254367/fullStaphylococcus aureusmembrane vesicleHldagrAinflammatory factor |
| spellingShingle | Juan Chen Yuhuan Lv Yuhuan Lv Weilong Shang Yi Yang Yuting Wang Zhen Hu Xiaonan Huang Rong Zhang Jizhen Yuan Jingbin Huang Xiancai Rao Loaded delta-hemolysin shapes the properties of Staphylococcus aureus membrane vesicles Frontiers in Microbiology Staphylococcus aureus membrane vesicle Hld agrA inflammatory factor |
| title | Loaded delta-hemolysin shapes the properties of Staphylococcus aureus membrane vesicles |
| title_full | Loaded delta-hemolysin shapes the properties of Staphylococcus aureus membrane vesicles |
| title_fullStr | Loaded delta-hemolysin shapes the properties of Staphylococcus aureus membrane vesicles |
| title_full_unstemmed | Loaded delta-hemolysin shapes the properties of Staphylococcus aureus membrane vesicles |
| title_short | Loaded delta-hemolysin shapes the properties of Staphylococcus aureus membrane vesicles |
| title_sort | loaded delta hemolysin shapes the properties of staphylococcus aureus membrane vesicles |
| topic | Staphylococcus aureus membrane vesicle Hld agrA inflammatory factor |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1254367/full |
| work_keys_str_mv | AT juanchen loadeddeltahemolysinshapesthepropertiesofstaphylococcusaureusmembranevesicles AT yuhuanlv loadeddeltahemolysinshapesthepropertiesofstaphylococcusaureusmembranevesicles AT yuhuanlv loadeddeltahemolysinshapesthepropertiesofstaphylococcusaureusmembranevesicles AT weilongshang loadeddeltahemolysinshapesthepropertiesofstaphylococcusaureusmembranevesicles AT yiyang loadeddeltahemolysinshapesthepropertiesofstaphylococcusaureusmembranevesicles AT yutingwang loadeddeltahemolysinshapesthepropertiesofstaphylococcusaureusmembranevesicles AT zhenhu loadeddeltahemolysinshapesthepropertiesofstaphylococcusaureusmembranevesicles AT xiaonanhuang loadeddeltahemolysinshapesthepropertiesofstaphylococcusaureusmembranevesicles AT rongzhang loadeddeltahemolysinshapesthepropertiesofstaphylococcusaureusmembranevesicles AT jizhenyuan loadeddeltahemolysinshapesthepropertiesofstaphylococcusaureusmembranevesicles AT jingbinhuang loadeddeltahemolysinshapesthepropertiesofstaphylococcusaureusmembranevesicles AT xiancairao loadeddeltahemolysinshapesthepropertiesofstaphylococcusaureusmembranevesicles |