Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells
Electrical stimulation is a well-known strategy for regulating cell behavior, both in pathological and physiological processes such as wound healing, tissue regeneration, and embryonic development. Electrotaxis is the directional migration of cells toward the cathode or anode when subjected to elect...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/11/2846 |
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| author | Mi Hee Lee Ye Jin Park Seung Hee Hong Min-Ah Koo Minyoung Cho Jong-Chul Park |
| author_facet | Mi Hee Lee Ye Jin Park Seung Hee Hong Min-Ah Koo Minyoung Cho Jong-Chul Park |
| author_sort | Mi Hee Lee |
| collection | DOAJ |
| description | Electrical stimulation is a well-known strategy for regulating cell behavior, both in pathological and physiological processes such as wound healing, tissue regeneration, and embryonic development. Electrotaxis is the directional migration of cells toward the cathode or anode when subjected to electrical stimulation. In this study, we investigated the conditions for enhanced directional migration of electrically stimulated adipose-derived stem cells (ADSCs) during prolonged culture, using a customized agar-salt electrotaxis chamber. Exposure of ADSCs to a 1200 μA electric current for 3 h, followed by cessation of stimulation for 6 h and resumed stimulation for a further 3 h, increased directional cell migration toward the anode without inducing cell death. Moreover, Golgi polarization maintained the direction of polarity parallel to the direction of cell movement. Herein, we demonstrated that a pulsed electric current is sufficient to trigger directional migration of ADSCs in long-term culture while maintaining cell viability. |
| first_indexed | 2024-03-10T05:37:06Z |
| format | Article |
| id | doaj.art-2579542c3778459c83afbc7fb6995dc6 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T05:37:06Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-2579542c3778459c83afbc7fb6995dc62023-11-22T22:47:36ZengMDPI AGCells2073-44092021-10-011011284610.3390/cells10112846Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem CellsMi Hee Lee0Ye Jin Park1Seung Hee Hong2Min-Ah Koo3Minyoung Cho4Jong-Chul Park5Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, KoreaCellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, KoreaCellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, KoreaCellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, KoreaCellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, KoreaCellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, KoreaElectrical stimulation is a well-known strategy for regulating cell behavior, both in pathological and physiological processes such as wound healing, tissue regeneration, and embryonic development. Electrotaxis is the directional migration of cells toward the cathode or anode when subjected to electrical stimulation. In this study, we investigated the conditions for enhanced directional migration of electrically stimulated adipose-derived stem cells (ADSCs) during prolonged culture, using a customized agar-salt electrotaxis chamber. Exposure of ADSCs to a 1200 μA electric current for 3 h, followed by cessation of stimulation for 6 h and resumed stimulation for a further 3 h, increased directional cell migration toward the anode without inducing cell death. Moreover, Golgi polarization maintained the direction of polarity parallel to the direction of cell movement. Herein, we demonstrated that a pulsed electric current is sufficient to trigger directional migration of ADSCs in long-term culture while maintaining cell viability.https://www.mdpi.com/2073-4409/10/11/2846electrotaxisdirectional migrationadipose-derived stem cellsGolgi polarization |
| spellingShingle | Mi Hee Lee Ye Jin Park Seung Hee Hong Min-Ah Koo Minyoung Cho Jong-Chul Park Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells Cells electrotaxis directional migration adipose-derived stem cells Golgi polarization |
| title | Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells |
| title_full | Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells |
| title_fullStr | Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells |
| title_full_unstemmed | Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells |
| title_short | Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells |
| title_sort | pulsed electrical stimulation enhances consistency of directional migration of adipose derived stem cells |
| topic | electrotaxis directional migration adipose-derived stem cells Golgi polarization |
| url | https://www.mdpi.com/2073-4409/10/11/2846 |
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