Ageing has no effect on the regulation of the ubiquitin proteasome-related genes and proteins following resistance exercise
Skeletal muscle atrophy is a critical component of the ageing process. Age-related muscle wasting is due to disrupted muscle protein turnover, a process mediated in part by the ubiquitin proteasome pathway (UPP). Additionally, older subjects have been observed to have an attenuated anabolic response...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-01-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00030/full |
| _version_ | 1811194748381167616 |
|---|---|
| author | Renae Jane Stefanetti Evelyn eZacharewicz Paul eDella Gatta Andrew eGarnham Aaron Paul Russell Severine eLamon |
| author_facet | Renae Jane Stefanetti Evelyn eZacharewicz Paul eDella Gatta Andrew eGarnham Aaron Paul Russell Severine eLamon |
| author_sort | Renae Jane Stefanetti |
| collection | DOAJ |
| description | Skeletal muscle atrophy is a critical component of the ageing process. Age-related muscle wasting is due to disrupted muscle protein turnover, a process mediated in part by the ubiquitin proteasome pathway (UPP). Additionally, older subjects have been observed to have an attenuated anabolic response, at both the molecular and physiological levels, following a single-bout of resistance exercise (RE). We investigated the expression levels of the UPP-related genes and proteins involved in muscle protein degradation in 10 older (60-75 years) versus 10 younger (18-30 years) healthy male subjects at basal as well as 2 hours after a single-bout of RE. MURF1, atrogin-1 and FBXO40, their substrate targets PKM2, myogenin, MYOD, MHC and EIF3F as well as MURF1 and atrogin-1 transcriptional regulators FOXO1 and FOXO3 gene and/or protein expression levels were measured via real time PCR and western blotting, respectively. At basal, no age-related difference was observed in the gene/protein levels of atrogin-1, MURF1, myogenin, MYOD and FOXO1/3. However, a decrease in FBXO40 mRNA and protein levels was observed in older subjects, while PKM2 protein was increased in older subjects. In response to RE, MURF1, atrogin-1 and FBXO40 mRNA were upregulated in both the younger and older subjects, with changes observed in protein levels. In conclusion, UPP-related gene/protein expression is comparably regulated in healthy young and old male subjects at basal and following RE. These findings suggest that UPP signalling plays a limited role in the process of age-related muscle wasting. Future studies are required to investigate additional proteolytic mechanisms in conjunction with skeletal muscle protein breakdown measurements following RE in older versus younger subjects. |
| first_indexed | 2024-04-12T00:31:46Z |
| format | Article |
| id | doaj.art-257aaa20f25746919233b690a983a5ad |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-12T00:31:46Z |
| publishDate | 2014-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-257aaa20f25746919233b690a983a5ad2022-12-22T03:55:18ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2014-01-01510.3389/fphys.2014.0003076676Ageing has no effect on the regulation of the ubiquitin proteasome-related genes and proteins following resistance exerciseRenae Jane Stefanetti0Evelyn eZacharewicz1Paul eDella Gatta2Andrew eGarnham3Aaron Paul Russell4Severine eLamon5Deakin UniversityDeakin UniversityDeakin UniversityDeakin UniversityDeakin UniversityDeakin UniversitySkeletal muscle atrophy is a critical component of the ageing process. Age-related muscle wasting is due to disrupted muscle protein turnover, a process mediated in part by the ubiquitin proteasome pathway (UPP). Additionally, older subjects have been observed to have an attenuated anabolic response, at both the molecular and physiological levels, following a single-bout of resistance exercise (RE). We investigated the expression levels of the UPP-related genes and proteins involved in muscle protein degradation in 10 older (60-75 years) versus 10 younger (18-30 years) healthy male subjects at basal as well as 2 hours after a single-bout of RE. MURF1, atrogin-1 and FBXO40, their substrate targets PKM2, myogenin, MYOD, MHC and EIF3F as well as MURF1 and atrogin-1 transcriptional regulators FOXO1 and FOXO3 gene and/or protein expression levels were measured via real time PCR and western blotting, respectively. At basal, no age-related difference was observed in the gene/protein levels of atrogin-1, MURF1, myogenin, MYOD and FOXO1/3. However, a decrease in FBXO40 mRNA and protein levels was observed in older subjects, while PKM2 protein was increased in older subjects. In response to RE, MURF1, atrogin-1 and FBXO40 mRNA were upregulated in both the younger and older subjects, with changes observed in protein levels. In conclusion, UPP-related gene/protein expression is comparably regulated in healthy young and old male subjects at basal and following RE. These findings suggest that UPP signalling plays a limited role in the process of age-related muscle wasting. Future studies are required to investigate additional proteolytic mechanisms in conjunction with skeletal muscle protein breakdown measurements following RE in older versus younger subjects.http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00030/fullSarcopeniaskeletal muscleubiquitin-proteasome systemResistance exercisemuscle protein breakdownatrogene signaling |
| spellingShingle | Renae Jane Stefanetti Evelyn eZacharewicz Paul eDella Gatta Andrew eGarnham Aaron Paul Russell Severine eLamon Ageing has no effect on the regulation of the ubiquitin proteasome-related genes and proteins following resistance exercise Frontiers in Physiology Sarcopenia skeletal muscle ubiquitin-proteasome system Resistance exercise muscle protein breakdown atrogene signaling |
| title | Ageing has no effect on the regulation of the ubiquitin proteasome-related genes and proteins following resistance exercise |
| title_full | Ageing has no effect on the regulation of the ubiquitin proteasome-related genes and proteins following resistance exercise |
| title_fullStr | Ageing has no effect on the regulation of the ubiquitin proteasome-related genes and proteins following resistance exercise |
| title_full_unstemmed | Ageing has no effect on the regulation of the ubiquitin proteasome-related genes and proteins following resistance exercise |
| title_short | Ageing has no effect on the regulation of the ubiquitin proteasome-related genes and proteins following resistance exercise |
| title_sort | ageing has no effect on the regulation of the ubiquitin proteasome related genes and proteins following resistance exercise |
| topic | Sarcopenia skeletal muscle ubiquitin-proteasome system Resistance exercise muscle protein breakdown atrogene signaling |
| url | http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00030/full |
| work_keys_str_mv | AT renaejanestefanetti ageinghasnoeffectontheregulationoftheubiquitinproteasomerelatedgenesandproteinsfollowingresistanceexercise AT evelynezacharewicz ageinghasnoeffectontheregulationoftheubiquitinproteasomerelatedgenesandproteinsfollowingresistanceexercise AT pauledellagatta ageinghasnoeffectontheregulationoftheubiquitinproteasomerelatedgenesandproteinsfollowingresistanceexercise AT andrewegarnham ageinghasnoeffectontheregulationoftheubiquitinproteasomerelatedgenesandproteinsfollowingresistanceexercise AT aaronpaulrussell ageinghasnoeffectontheregulationoftheubiquitinproteasomerelatedgenesandproteinsfollowingresistanceexercise AT severineelamon ageinghasnoeffectontheregulationoftheubiquitinproteasomerelatedgenesandproteinsfollowingresistanceexercise |