Nanosecond Laser–Fabricated Monolayer of Gold Nanoparticles on ITO for Bioelectrocatalysis
Redox enzymes can be envisioned as biocatalysts in various electrocatalytic-based devices. Among factors that play roles in bioelectrochemistry limitations, the effect of enzyme-enzyme neighboring interaction on electrocatalysis has rarely been investigated, although critical in vivo. We report in t...
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fchem.2020.00431/full |
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| author | Vivek Pratap Hitaishi Ievgen Mazurenko Anjali Vengasseril Murali Anne de Poulpiquet Gaëlle Coustillier Philippe Delaporte Elisabeth Lojou |
| author_facet | Vivek Pratap Hitaishi Ievgen Mazurenko Anjali Vengasseril Murali Anne de Poulpiquet Gaëlle Coustillier Philippe Delaporte Elisabeth Lojou |
| author_sort | Vivek Pratap Hitaishi |
| collection | DOAJ |
| description | Redox enzymes can be envisioned as biocatalysts in various electrocatalytic-based devices. Among factors that play roles in bioelectrochemistry limitations, the effect of enzyme-enzyme neighboring interaction on electrocatalysis has rarely been investigated, although critical in vivo. We report in this work an in-depth study of gold nanoparticles prepared by laser ablation in the ultimate goal of determining the relationship between activity and enzyme density on electrodes. Nanosecond laser interaction with nanometric gold films deposited on indium tin oxide support was used to generate in situ gold nanoparticles (AuNPs) free from any stabilizers. A comprehensive analysis of AuNP size and coverage, as well as total geometric surface vs. electroactive surface is provided as a function of the thickness of the treated gold layer. Using microscopy and electrochemistry, the long-term stability of AuNP-based electrodes in the atmosphere and in the electrolyte is demonstrated. AuNPs formed by laser treatment are then modified by thiol chemistry and their electrochemical behavior is tested with a redox probe. Finally, enzyme adsorption and bioelectrocatalysis are evaluated in the case of two enzymes, i.e., the Myrothecium verrucaria bilirubin oxidase and the Thermus thermophilus laccase. Behaving differently on charged surfaces, they allow demonstrating the validity of laser treated AuNPs for bioelectrocatalysis. |
| first_indexed | 2024-12-12T22:56:32Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-12T22:56:32Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-257d50080e9b443d93a7589dedbfde602022-12-22T00:08:56ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462020-06-01810.3389/fchem.2020.00431547150Nanosecond Laser–Fabricated Monolayer of Gold Nanoparticles on ITO for BioelectrocatalysisVivek Pratap Hitaishi0Ievgen Mazurenko1Anjali Vengasseril Murali2Anne de Poulpiquet3Gaëlle Coustillier4Philippe Delaporte5Elisabeth Lojou6Aix Marseille Univ, CNRS, BIP, Bioénergétique et Ingénierie des Protéines, UMR 7281, Marseille, FranceAix Marseille Univ, CNRS, BIP, Bioénergétique et Ingénierie des Protéines, UMR 7281, Marseille, FranceAix Marseille Univ, CNRS, LP3, UMR 7341, Parc Scientifique et Technologique de Luminy, Marseille, FranceAix Marseille Univ, CNRS, BIP, Bioénergétique et Ingénierie des Protéines, UMR 7281, Marseille, FranceAix Marseille Univ, CNRS, LP3, UMR 7341, Parc Scientifique et Technologique de Luminy, Marseille, FranceAix Marseille Univ, CNRS, LP3, UMR 7341, Parc Scientifique et Technologique de Luminy, Marseille, FranceAix Marseille Univ, CNRS, BIP, Bioénergétique et Ingénierie des Protéines, UMR 7281, Marseille, FranceRedox enzymes can be envisioned as biocatalysts in various electrocatalytic-based devices. Among factors that play roles in bioelectrochemistry limitations, the effect of enzyme-enzyme neighboring interaction on electrocatalysis has rarely been investigated, although critical in vivo. We report in this work an in-depth study of gold nanoparticles prepared by laser ablation in the ultimate goal of determining the relationship between activity and enzyme density on electrodes. Nanosecond laser interaction with nanometric gold films deposited on indium tin oxide support was used to generate in situ gold nanoparticles (AuNPs) free from any stabilizers. A comprehensive analysis of AuNP size and coverage, as well as total geometric surface vs. electroactive surface is provided as a function of the thickness of the treated gold layer. Using microscopy and electrochemistry, the long-term stability of AuNP-based electrodes in the atmosphere and in the electrolyte is demonstrated. AuNPs formed by laser treatment are then modified by thiol chemistry and their electrochemical behavior is tested with a redox probe. Finally, enzyme adsorption and bioelectrocatalysis are evaluated in the case of two enzymes, i.e., the Myrothecium verrucaria bilirubin oxidase and the Thermus thermophilus laccase. Behaving differently on charged surfaces, they allow demonstrating the validity of laser treated AuNPs for bioelectrocatalysis.https://www.frontiersin.org/article/10.3389/fchem.2020.00431/fulllasernanoparticleselectrode nanostructurationenzymescatalysisself-assembled-monolayers |
| spellingShingle | Vivek Pratap Hitaishi Ievgen Mazurenko Anjali Vengasseril Murali Anne de Poulpiquet Gaëlle Coustillier Philippe Delaporte Elisabeth Lojou Nanosecond Laser–Fabricated Monolayer of Gold Nanoparticles on ITO for Bioelectrocatalysis Frontiers in Chemistry laser nanoparticles electrode nanostructuration enzymes catalysis self-assembled-monolayers |
| title | Nanosecond Laser–Fabricated Monolayer of Gold Nanoparticles on ITO for Bioelectrocatalysis |
| title_full | Nanosecond Laser–Fabricated Monolayer of Gold Nanoparticles on ITO for Bioelectrocatalysis |
| title_fullStr | Nanosecond Laser–Fabricated Monolayer of Gold Nanoparticles on ITO for Bioelectrocatalysis |
| title_full_unstemmed | Nanosecond Laser–Fabricated Monolayer of Gold Nanoparticles on ITO for Bioelectrocatalysis |
| title_short | Nanosecond Laser–Fabricated Monolayer of Gold Nanoparticles on ITO for Bioelectrocatalysis |
| title_sort | nanosecond laser fabricated monolayer of gold nanoparticles on ito for bioelectrocatalysis |
| topic | laser nanoparticles electrode nanostructuration enzymes catalysis self-assembled-monolayers |
| url | https://www.frontiersin.org/article/10.3389/fchem.2020.00431/full |
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