Neuroprotective Effects of a New Derivative of Chlojaponilactone B against Oxidative Damaged Induced by Hydrogen Peroxide in PC12 Cells
A new sesquiterpenoid (<b>1</b>) was obtained by hydrogenating Chlojaponilactone B. The structure of <b>1</b> was elucidated according to a combination of NMR, HRESIMS, and NOE diffraction data. The treatment of H<sub>2</sub>O<sub>2</sub> in a PC12 cel...
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MDPI AG
2022-09-01
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| Serija: | Molecules |
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| Online pristup: | https://www.mdpi.com/1420-3049/27/18/6049 |
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| author | Shaoxia Ye Qiyin Wen Longping Zhu Chunguo Qian Depo Yang Zhimin Zhao |
| author_facet | Shaoxia Ye Qiyin Wen Longping Zhu Chunguo Qian Depo Yang Zhimin Zhao |
| author_sort | Shaoxia Ye |
| collection | DOAJ |
| description | A new sesquiterpenoid (<b>1</b>) was obtained by hydrogenating Chlojaponilactone B. The structure of <b>1</b> was elucidated according to a combination of NMR, HRESIMS, and NOE diffraction data. The treatment of H<sub>2</sub>O<sub>2</sub> in a PC12 cell model was used to evaluate the antioxidant activity of <b>1</b>. An MMT assay showed that <b>1</b> had no cytotoxicity to the PC12 cell and rescued cell viability from the oxidative damage caused by H<sub>2</sub>O<sub>2</sub>. The treatment of <b>1</b> stabilized the mitochondria membrane potential (MMP), which decreased the intracellular ROS level and reduced cell apoptosis in the oxidative stress model. The activities of antioxidant enzyme superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of intracellular glutathione (GSH) were significantly enhanced after the treatment of <b>1</b>. In addition, the results of qRT-PCR showed that <b>1</b> treatment minimized the cell injury by H<sub>2</sub>O<sub>2</sub> via the up-regulation of the expression of nuclear factor erythroid 2 (Nrf2) and its downstream enzymes Heme oxygenase 1 (HO-1), glutamate cysteine ligase-modifier subunit (GCLm), and NAD(P)H quinone dehydrogenase 1 (Nqo1). Based on the antioxidant activity of <b>1</b>, we speculated its potential as a therapeutic agent for some diseases induced by oxidative damage. |
| first_indexed | 2024-03-09T22:59:47Z |
| format | Article |
| id | doaj.art-257e7af2cf75459684a5dcd4581a31ab |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T22:59:47Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-257e7af2cf75459684a5dcd4581a31ab2023-11-23T18:03:24ZengMDPI AGMolecules1420-30492022-09-012718604910.3390/molecules27186049Neuroprotective Effects of a New Derivative of Chlojaponilactone B against Oxidative Damaged Induced by Hydrogen Peroxide in PC12 CellsShaoxia Ye0Qiyin Wen1Longping Zhu2Chunguo Qian3Depo Yang4Zhimin Zhao5School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaA new sesquiterpenoid (<b>1</b>) was obtained by hydrogenating Chlojaponilactone B. The structure of <b>1</b> was elucidated according to a combination of NMR, HRESIMS, and NOE diffraction data. The treatment of H<sub>2</sub>O<sub>2</sub> in a PC12 cell model was used to evaluate the antioxidant activity of <b>1</b>. An MMT assay showed that <b>1</b> had no cytotoxicity to the PC12 cell and rescued cell viability from the oxidative damage caused by H<sub>2</sub>O<sub>2</sub>. The treatment of <b>1</b> stabilized the mitochondria membrane potential (MMP), which decreased the intracellular ROS level and reduced cell apoptosis in the oxidative stress model. The activities of antioxidant enzyme superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of intracellular glutathione (GSH) were significantly enhanced after the treatment of <b>1</b>. In addition, the results of qRT-PCR showed that <b>1</b> treatment minimized the cell injury by H<sub>2</sub>O<sub>2</sub> via the up-regulation of the expression of nuclear factor erythroid 2 (Nrf2) and its downstream enzymes Heme oxygenase 1 (HO-1), glutamate cysteine ligase-modifier subunit (GCLm), and NAD(P)H quinone dehydrogenase 1 (Nqo1). Based on the antioxidant activity of <b>1</b>, we speculated its potential as a therapeutic agent for some diseases induced by oxidative damage.https://www.mdpi.com/1420-3049/27/18/6049sesquiterpeneneuroprotectionPC12 cellsROSNrf2 |
| spellingShingle | Shaoxia Ye Qiyin Wen Longping Zhu Chunguo Qian Depo Yang Zhimin Zhao Neuroprotective Effects of a New Derivative of Chlojaponilactone B against Oxidative Damaged Induced by Hydrogen Peroxide in PC12 Cells Molecules sesquiterpene neuroprotection PC12 cells ROS Nrf2 |
| title | Neuroprotective Effects of a New Derivative of Chlojaponilactone B against Oxidative Damaged Induced by Hydrogen Peroxide in PC12 Cells |
| title_full | Neuroprotective Effects of a New Derivative of Chlojaponilactone B against Oxidative Damaged Induced by Hydrogen Peroxide in PC12 Cells |
| title_fullStr | Neuroprotective Effects of a New Derivative of Chlojaponilactone B against Oxidative Damaged Induced by Hydrogen Peroxide in PC12 Cells |
| title_full_unstemmed | Neuroprotective Effects of a New Derivative of Chlojaponilactone B against Oxidative Damaged Induced by Hydrogen Peroxide in PC12 Cells |
| title_short | Neuroprotective Effects of a New Derivative of Chlojaponilactone B against Oxidative Damaged Induced by Hydrogen Peroxide in PC12 Cells |
| title_sort | neuroprotective effects of a new derivative of chlojaponilactone b against oxidative damaged induced by hydrogen peroxide in pc12 cells |
| topic | sesquiterpene neuroprotection PC12 cells ROS Nrf2 |
| url | https://www.mdpi.com/1420-3049/27/18/6049 |
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