Over-Produced Extracellular Polymeric Substances and Activated Antioxidant Enzymes Attribute to Resistance of Pb(II) for Algal–Bacterial Granular Sludge in Municipal Wastewater Treatment
Algal–bacterial granular sludge technology is a new type of wastewater treatment and resource regeneration process, which has received widespread attention due to its excellent nitrogen and phosphorus removal performance, and energy-saving and emission reduction effects. Although algal–bacterial gra...
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/2073-4441/15/21/3833 |
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| author | Juanru Yang Yu Zhang Shulian Wang |
| author_facet | Juanru Yang Yu Zhang Shulian Wang |
| author_sort | Juanru Yang |
| collection | DOAJ |
| description | Algal–bacterial granular sludge technology is a new type of wastewater treatment and resource regeneration process, which has received widespread attention due to its excellent nitrogen and phosphorus removal performance, and energy-saving and emission reduction effects. Although algal–bacterial granular sludge technology has achieved an ideal nutrient removal ability, some pol-lutants in wastewater might affect the symbiotic relationship between algae and bacteria. This study investigated the impact of coexisting Pb(II) on the symbiosis of algal–bacterial granular sludge. It was found that 2.5–10.0 mg/L of Pb(II) exposure increased the relative abundance of Pro-teobacteria. In addition, more protein in extracellular polymeric substances (EPS-PN) was secreted at 2.5 mg/L of Pb(II) exposure while EPS-PN content reduced at a rate of 5.0–10.0 mg/L of Pb(II). Under different concentrations of Pb(II), the damage degree of algal–bacterial granular sludge was exacerbated, evidenced by increased malondialdehyde (MDA) content. To cope with these adverse circumstances, the antioxidant enzyme activity of both super-oxide dismutase (SOD) and peroxidase dismutase (CAT) was boosted. With the help of these adaptive strategies, the symbiosis of algal–bacterial granular sludge was stable. Moreover, the performance of algal–bacterial granular sludge in treating COD, ammonia-N and phosphate-P was kept at above 95%. This study approved that a Pb(II) concentration less than 10.0 mg/L had little effect on the performance of algal–bacterial granular sludge in wastewater treatment. It is hoped that this study can provide useful information for an improved engineering feasibility of algal–bacterial granular sludge process. |
| first_indexed | 2024-03-11T11:18:11Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-11T11:18:11Z |
| publishDate | 2023-11-01 |
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| series | Water |
| spelling | doaj.art-25804f4a766c43b7a7e64766686926a02023-11-10T15:15:29ZengMDPI AGWater2073-44412023-11-011521383310.3390/w15213833Over-Produced Extracellular Polymeric Substances and Activated Antioxidant Enzymes Attribute to Resistance of Pb(II) for Algal–Bacterial Granular Sludge in Municipal Wastewater TreatmentJuanru Yang0Yu Zhang1Shulian Wang2School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068, ChinaSchool of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068, ChinaKey Laboratory of Health Intelligent Perception and Ecological Restoration of River and Lake, Ministry of Education, Hubei University of Technology, Wuhan 430068, ChinaAlgal–bacterial granular sludge technology is a new type of wastewater treatment and resource regeneration process, which has received widespread attention due to its excellent nitrogen and phosphorus removal performance, and energy-saving and emission reduction effects. Although algal–bacterial granular sludge technology has achieved an ideal nutrient removal ability, some pol-lutants in wastewater might affect the symbiotic relationship between algae and bacteria. This study investigated the impact of coexisting Pb(II) on the symbiosis of algal–bacterial granular sludge. It was found that 2.5–10.0 mg/L of Pb(II) exposure increased the relative abundance of Pro-teobacteria. In addition, more protein in extracellular polymeric substances (EPS-PN) was secreted at 2.5 mg/L of Pb(II) exposure while EPS-PN content reduced at a rate of 5.0–10.0 mg/L of Pb(II). Under different concentrations of Pb(II), the damage degree of algal–bacterial granular sludge was exacerbated, evidenced by increased malondialdehyde (MDA) content. To cope with these adverse circumstances, the antioxidant enzyme activity of both super-oxide dismutase (SOD) and peroxidase dismutase (CAT) was boosted. With the help of these adaptive strategies, the symbiosis of algal–bacterial granular sludge was stable. Moreover, the performance of algal–bacterial granular sludge in treating COD, ammonia-N and phosphate-P was kept at above 95%. This study approved that a Pb(II) concentration less than 10.0 mg/L had little effect on the performance of algal–bacterial granular sludge in wastewater treatment. It is hoped that this study can provide useful information for an improved engineering feasibility of algal–bacterial granular sludge process.https://www.mdpi.com/2073-4441/15/21/3833algal–bacterial granular sludgedivalent lead ionextracellular polymeric substanceantioxidant enzymeresistance |
| spellingShingle | Juanru Yang Yu Zhang Shulian Wang Over-Produced Extracellular Polymeric Substances and Activated Antioxidant Enzymes Attribute to Resistance of Pb(II) for Algal–Bacterial Granular Sludge in Municipal Wastewater Treatment Water algal–bacterial granular sludge divalent lead ion extracellular polymeric substance antioxidant enzyme resistance |
| title | Over-Produced Extracellular Polymeric Substances and Activated Antioxidant Enzymes Attribute to Resistance of Pb(II) for Algal–Bacterial Granular Sludge in Municipal Wastewater Treatment |
| title_full | Over-Produced Extracellular Polymeric Substances and Activated Antioxidant Enzymes Attribute to Resistance of Pb(II) for Algal–Bacterial Granular Sludge in Municipal Wastewater Treatment |
| title_fullStr | Over-Produced Extracellular Polymeric Substances and Activated Antioxidant Enzymes Attribute to Resistance of Pb(II) for Algal–Bacterial Granular Sludge in Municipal Wastewater Treatment |
| title_full_unstemmed | Over-Produced Extracellular Polymeric Substances and Activated Antioxidant Enzymes Attribute to Resistance of Pb(II) for Algal–Bacterial Granular Sludge in Municipal Wastewater Treatment |
| title_short | Over-Produced Extracellular Polymeric Substances and Activated Antioxidant Enzymes Attribute to Resistance of Pb(II) for Algal–Bacterial Granular Sludge in Municipal Wastewater Treatment |
| title_sort | over produced extracellular polymeric substances and activated antioxidant enzymes attribute to resistance of pb ii for algal bacterial granular sludge in municipal wastewater treatment |
| topic | algal–bacterial granular sludge divalent lead ion extracellular polymeric substance antioxidant enzyme resistance |
| url | https://www.mdpi.com/2073-4441/15/21/3833 |
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