Effects of selective RNA processing and stabilization enzymes on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002
Synechococcus is one of the most abundant prokaryotic photosynthetic organisms on Earth and plays a key role in oceanic carbon fixation and transformation. To improve the photosynthetic efficiency of synechococcus, a post-transcriptional regulatory mechanism - Selective RNA Processing and Stabilizat...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1223060/full |
| _version_ | 1827910097885462528 |
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| author | Jinyu Chen Daixi Liu Yafei Wang Shaoyu Wang Ranran Huang |
| author_facet | Jinyu Chen Daixi Liu Yafei Wang Shaoyu Wang Ranran Huang |
| author_sort | Jinyu Chen |
| collection | DOAJ |
| description | Synechococcus is one of the most abundant prokaryotic photosynthetic organisms on Earth and plays a key role in oceanic carbon fixation and transformation. To improve the photosynthetic efficiency of synechococcus, a post-transcriptional regulatory mechanism - Selective RNA Processing and Stabilization (SRPS) was considered. We inactivated the SRPS-enzymes, executor of the SRPS mechanism, to explore their regulation rule of photosynthetic carbon fixation efficiency in Synechococcus. The results showed that the inactivation of SRPS-enzymes mainly affected the growth rate or growth phase. It significantly alters the photosynthetic oxygen evolution rate, pigment content, chlorophyll fluorescence, carbon and nitrogen content, as well as the composition and biological activity of the dissolved organic matter derived from Synechococcus (SOM). Inactivating SRPS-enzymes results in an increase in the expression level of most subunits of the Cytochrome b6-f complex, while the expression levels of most subunits of PSI, PSII, RuBisCO, and NDH decrease. All SRPS-enzymes are involved in the expression regulation of basilic protein complexes in photosynthesis, such as PSI, PSII, Cytochrome b6-f complex, ATP synthase, and RuBisCO. Our results indicate that the inactivation of SRPS-enzymes have a significant influence on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002. |
| first_indexed | 2024-03-13T01:47:18Z |
| format | Article |
| id | doaj.art-2583f2298f22414d9e9566f8cc456bb6 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-03-13T01:47:18Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-2583f2298f22414d9e9566f8cc456bb62023-07-03T06:10:24ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-07-011010.3389/fmars.2023.12230601223060Effects of selective RNA processing and stabilization enzymes on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002Jinyu Chen0Daixi Liu1Yafei Wang2Shaoyu Wang3Ranran Huang4Institute of Marine Science and Technology, Shandong University, Qingdao, ChinaSchool of Pharmaceutical Sciences, Shandong University, Jinan, ChinaInstitute of Marine Science and Technology, Shandong University, Qingdao, ChinaInstitute of Marine Science and Technology, Shandong University, Qingdao, ChinaInstitute of Marine Science and Technology, Shandong University, Qingdao, ChinaSynechococcus is one of the most abundant prokaryotic photosynthetic organisms on Earth and plays a key role in oceanic carbon fixation and transformation. To improve the photosynthetic efficiency of synechococcus, a post-transcriptional regulatory mechanism - Selective RNA Processing and Stabilization (SRPS) was considered. We inactivated the SRPS-enzymes, executor of the SRPS mechanism, to explore their regulation rule of photosynthetic carbon fixation efficiency in Synechococcus. The results showed that the inactivation of SRPS-enzymes mainly affected the growth rate or growth phase. It significantly alters the photosynthetic oxygen evolution rate, pigment content, chlorophyll fluorescence, carbon and nitrogen content, as well as the composition and biological activity of the dissolved organic matter derived from Synechococcus (SOM). Inactivating SRPS-enzymes results in an increase in the expression level of most subunits of the Cytochrome b6-f complex, while the expression levels of most subunits of PSI, PSII, RuBisCO, and NDH decrease. All SRPS-enzymes are involved in the expression regulation of basilic protein complexes in photosynthesis, such as PSI, PSII, Cytochrome b6-f complex, ATP synthase, and RuBisCO. Our results indicate that the inactivation of SRPS-enzymes have a significant influence on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002.https://www.frontiersin.org/articles/10.3389/fmars.2023.1223060/fullSynechococcus sp. PCC 7002selective RNA processing and stabilization (SRPS)photosynthetic carbon fixationdissolved organic matter derived from Synechococcus (SOM)splice site |
| spellingShingle | Jinyu Chen Daixi Liu Yafei Wang Shaoyu Wang Ranran Huang Effects of selective RNA processing and stabilization enzymes on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002 Frontiers in Marine Science Synechococcus sp. PCC 7002 selective RNA processing and stabilization (SRPS) photosynthetic carbon fixation dissolved organic matter derived from Synechococcus (SOM) splice site |
| title | Effects of selective RNA processing and stabilization enzymes on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002 |
| title_full | Effects of selective RNA processing and stabilization enzymes on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002 |
| title_fullStr | Effects of selective RNA processing and stabilization enzymes on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002 |
| title_full_unstemmed | Effects of selective RNA processing and stabilization enzymes on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002 |
| title_short | Effects of selective RNA processing and stabilization enzymes on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002 |
| title_sort | effects of selective rna processing and stabilization enzymes on carbon sequestration by photosynthesis of synechococcus sp pcc7002 |
| topic | Synechococcus sp. PCC 7002 selective RNA processing and stabilization (SRPS) photosynthetic carbon fixation dissolved organic matter derived from Synechococcus (SOM) splice site |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1223060/full |
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