The Poplar (<i>Populus trichocarpa</i>) Dehydrin Gene <i>PtrDHN-3</i> Enhances Tolerance to Salt Stress in Arabidopsis
Dehydrin (DHN), a member of the late embryogenesis abundant protein (LEA) family, was recently found to play a role in physiological responses to salt and drought stress. In this study, we identified and cloned the <i>PtrDHN-3</i> gene from <i>Populus trichocarpa</i>. The Ptr...
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MDPI AG
2022-10-01
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| author | Meiqi Zhou Nafei Peng Chuanping Yang Chao Wang |
| author_facet | Meiqi Zhou Nafei Peng Chuanping Yang Chao Wang |
| author_sort | Meiqi Zhou |
| collection | DOAJ |
| description | Dehydrin (DHN), a member of the late embryogenesis abundant protein (LEA) family, was recently found to play a role in physiological responses to salt and drought stress. In this study, we identified and cloned the <i>PtrDHN-3</i> gene from <i>Populus trichocarpa</i>. The PtrDHN-3 protein encoded 226 amino acids, having a molecular weight of 25.78 KDa and an isoelectric point of 5.18. It was identified as a SKn-type DHN and was clustered with other resistance-related DHN proteins. Real-time fluorescent quantitative PCR showed that transcription levels of <i>PtrDHN-3</i> were induced by mannitol stress, and more significantly by salt stress. Meanwhile, in a yeast transgenic assay, salt tolerance increased in the <i>PtrDHN-3</i> transgenic yeast, while the germination rate, fresh weight and chlorophyll content increased in <i>PtrDHN-3</i>-overexpressing transgenic <i>Arabidopsis</i> plants (OE) under salt stress. Significant increases in expression levels of six antioxidant enzymes genes, and SOD and POD enzyme activity was also observed in the OE lines, resulting in a decrease in O<sub>2</sub><sup>-</sup> and H<sub>2</sub>O<sub>2</sub> accumulation. The proline content also increased significantly compared with the wild-type, along with expression of proline synthesis-related genes <i>P5CS1</i> and <i>P5CS2</i>. These findings suggest that <i>PtrDHN-3</i> plays an important role in salt resistance in plants. |
| first_indexed | 2024-03-09T19:33:21Z |
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| last_indexed | 2024-03-09T19:33:21Z |
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| spelling | doaj.art-258948bc7e5643d0af2513b07222df372023-11-24T02:03:47ZengMDPI AGPlants2223-77472022-10-011120270010.3390/plants11202700The Poplar (<i>Populus trichocarpa</i>) Dehydrin Gene <i>PtrDHN-3</i> Enhances Tolerance to Salt Stress in ArabidopsisMeiqi Zhou0Nafei Peng1Chuanping Yang2Chao Wang3State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaDehydrin (DHN), a member of the late embryogenesis abundant protein (LEA) family, was recently found to play a role in physiological responses to salt and drought stress. In this study, we identified and cloned the <i>PtrDHN-3</i> gene from <i>Populus trichocarpa</i>. The PtrDHN-3 protein encoded 226 amino acids, having a molecular weight of 25.78 KDa and an isoelectric point of 5.18. It was identified as a SKn-type DHN and was clustered with other resistance-related DHN proteins. Real-time fluorescent quantitative PCR showed that transcription levels of <i>PtrDHN-3</i> were induced by mannitol stress, and more significantly by salt stress. Meanwhile, in a yeast transgenic assay, salt tolerance increased in the <i>PtrDHN-3</i> transgenic yeast, while the germination rate, fresh weight and chlorophyll content increased in <i>PtrDHN-3</i>-overexpressing transgenic <i>Arabidopsis</i> plants (OE) under salt stress. Significant increases in expression levels of six antioxidant enzymes genes, and SOD and POD enzyme activity was also observed in the OE lines, resulting in a decrease in O<sub>2</sub><sup>-</sup> and H<sub>2</sub>O<sub>2</sub> accumulation. The proline content also increased significantly compared with the wild-type, along with expression of proline synthesis-related genes <i>P5CS1</i> and <i>P5CS2</i>. These findings suggest that <i>PtrDHN-3</i> plays an important role in salt resistance in plants.https://www.mdpi.com/2223-7747/11/20/2700<i>Populus trichocarpa</i><i>PtrDHN-3</i>salt stressROS-scavenging capability |
| spellingShingle | Meiqi Zhou Nafei Peng Chuanping Yang Chao Wang The Poplar (<i>Populus trichocarpa</i>) Dehydrin Gene <i>PtrDHN-3</i> Enhances Tolerance to Salt Stress in Arabidopsis Plants <i>Populus trichocarpa</i> <i>PtrDHN-3</i> salt stress ROS-scavenging capability |
| title | The Poplar (<i>Populus trichocarpa</i>) Dehydrin Gene <i>PtrDHN-3</i> Enhances Tolerance to Salt Stress in Arabidopsis |
| title_full | The Poplar (<i>Populus trichocarpa</i>) Dehydrin Gene <i>PtrDHN-3</i> Enhances Tolerance to Salt Stress in Arabidopsis |
| title_fullStr | The Poplar (<i>Populus trichocarpa</i>) Dehydrin Gene <i>PtrDHN-3</i> Enhances Tolerance to Salt Stress in Arabidopsis |
| title_full_unstemmed | The Poplar (<i>Populus trichocarpa</i>) Dehydrin Gene <i>PtrDHN-3</i> Enhances Tolerance to Salt Stress in Arabidopsis |
| title_short | The Poplar (<i>Populus trichocarpa</i>) Dehydrin Gene <i>PtrDHN-3</i> Enhances Tolerance to Salt Stress in Arabidopsis |
| title_sort | poplar i populus trichocarpa i dehydrin gene i ptrdhn 3 i enhances tolerance to salt stress in arabidopsis |
| topic | <i>Populus trichocarpa</i> <i>PtrDHN-3</i> salt stress ROS-scavenging capability |
| url | https://www.mdpi.com/2223-7747/11/20/2700 |
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