Differential Expression of miRNA-223 in Coronary In-Stent Restenosis
Objective: In-stent restenosis (ISR) is an unfavorable complication that occurs in patients after coronary stenting. Despite the progress with advent of modern DES and new antiplatelet agents, restenosis still hampers PCI short- and long-term results. The aim of this study was to investigate whether...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/3/849 |
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| author | Shiva Ganjali Seyed Hamid Aghaee-Bakhtiari Željko Reiner Amirhossein Sahebkar |
| author_facet | Shiva Ganjali Seyed Hamid Aghaee-Bakhtiari Željko Reiner Amirhossein Sahebkar |
| author_sort | Shiva Ganjali |
| collection | DOAJ |
| description | Objective: In-stent restenosis (ISR) is an unfavorable complication that occurs in patients after coronary stenting. Despite the progress with advent of modern DES and new antiplatelet agents, restenosis still hampers PCI short- and long-term results. The aim of this study was to investigate whether circulating miRNA-223, which is associated with HDL particles and involved in cholesterol efflux pathway, have diagnostic capability for determining ISR. Methods: This case–control study comprised 21 ISR and 26 NISR patients. The level of miRNA-223 expression was evaluated by TaqMan Real-Time PCR, quantified by the comparative method (fold change) and normalized to U6 expression. Results: Patients in ISR and NISR groups were not different in terms of demographic, clinical, and biochemical parameters, except that the percentage of patients who had DES was significantly greater in the NISR group (88.9%) in comparison with the ISR group (50%). The serum expression of miRNA-223 in ISR patients was 3.277 ± 0.9 times greater than that in NISR group (<i>p</i> = 0.016). In addition, the results of binary logistic regression demonstrated that the high level of serum miRNA-223 was strongly and positively associated with the ISR risk (OR: 17.818, 95% CI: 1.115–284.623, <i>p</i> = 0.042) after adjustment for age, sex, HDL-C, LDL-C, FBS, and statin consumption. Conclusion: Elevated serum level of miRNA-223 might be helpful in predicting the occurrence of ISR. Further confirmation in future large-scale studies is warranted. |
| first_indexed | 2024-03-09T23:38:42Z |
| format | Article |
| id | doaj.art-258a42143c9d40ccbc36325375add56f |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T23:38:42Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-258a42143c9d40ccbc36325375add56f2023-11-23T16:54:59ZengMDPI AGJournal of Clinical Medicine2077-03832022-02-0111384910.3390/jcm11030849Differential Expression of miRNA-223 in Coronary In-Stent RestenosisShiva Ganjali0Seyed Hamid Aghaee-Bakhtiari1Željko Reiner2Amirhossein Sahebkar3Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, IranBioinformatics Research Group, Mashhad University of Medical Sciences, Mashhad 9177948564, IranDepartment of Internal Medicine, School of Medicine, University Hospital Center Zagreb, University of Zagreb, 10000 Zagreb, CroatiaApplied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad 9177948564, IranObjective: In-stent restenosis (ISR) is an unfavorable complication that occurs in patients after coronary stenting. Despite the progress with advent of modern DES and new antiplatelet agents, restenosis still hampers PCI short- and long-term results. The aim of this study was to investigate whether circulating miRNA-223, which is associated with HDL particles and involved in cholesterol efflux pathway, have diagnostic capability for determining ISR. Methods: This case–control study comprised 21 ISR and 26 NISR patients. The level of miRNA-223 expression was evaluated by TaqMan Real-Time PCR, quantified by the comparative method (fold change) and normalized to U6 expression. Results: Patients in ISR and NISR groups were not different in terms of demographic, clinical, and biochemical parameters, except that the percentage of patients who had DES was significantly greater in the NISR group (88.9%) in comparison with the ISR group (50%). The serum expression of miRNA-223 in ISR patients was 3.277 ± 0.9 times greater than that in NISR group (<i>p</i> = 0.016). In addition, the results of binary logistic regression demonstrated that the high level of serum miRNA-223 was strongly and positively associated with the ISR risk (OR: 17.818, 95% CI: 1.115–284.623, <i>p</i> = 0.042) after adjustment for age, sex, HDL-C, LDL-C, FBS, and statin consumption. Conclusion: Elevated serum level of miRNA-223 might be helpful in predicting the occurrence of ISR. Further confirmation in future large-scale studies is warranted.https://www.mdpi.com/2077-0383/11/3/849in-stent restenosisHDL-associated miRNAmiRNA-223 |
| spellingShingle | Shiva Ganjali Seyed Hamid Aghaee-Bakhtiari Željko Reiner Amirhossein Sahebkar Differential Expression of miRNA-223 in Coronary In-Stent Restenosis Journal of Clinical Medicine in-stent restenosis HDL-associated miRNA miRNA-223 |
| title | Differential Expression of miRNA-223 in Coronary In-Stent Restenosis |
| title_full | Differential Expression of miRNA-223 in Coronary In-Stent Restenosis |
| title_fullStr | Differential Expression of miRNA-223 in Coronary In-Stent Restenosis |
| title_full_unstemmed | Differential Expression of miRNA-223 in Coronary In-Stent Restenosis |
| title_short | Differential Expression of miRNA-223 in Coronary In-Stent Restenosis |
| title_sort | differential expression of mirna 223 in coronary in stent restenosis |
| topic | in-stent restenosis HDL-associated miRNA miRNA-223 |
| url | https://www.mdpi.com/2077-0383/11/3/849 |
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