Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances

The sludge produced in wastewater treatment plants of slaughterhouses is a rich source of chemical, organic, and microbiological constituents that can be biotechnologically exploited. The purpose of this study was to (i) conduct a chemical analysis of the sludge, and (ii) isolate, quantify, and desc...

Full description

Bibliographic Details
Main Authors: Jorge Avelino Rodriguez Lozada, Klever Cristiano Silveira, Libério Junio da Silva, Marihus Altoé Baldotto, Lílian Estrela Borges Baldotto
Format: Article
Language:English
Published: Universidade Estadual de Londrina 2017-06-01
Series:Semina: Ciências Agrárias
Subjects:
Online Access:http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/25953
_version_ 1811264435662094336
author Jorge Avelino Rodriguez Lozada
Klever Cristiano Silveira
Libério Junio da Silva
Marihus Altoé Baldotto
Lílian Estrela Borges Baldotto
author_facet Jorge Avelino Rodriguez Lozada
Klever Cristiano Silveira
Libério Junio da Silva
Marihus Altoé Baldotto
Lílian Estrela Borges Baldotto
author_sort Jorge Avelino Rodriguez Lozada
collection DOAJ
description The sludge produced in wastewater treatment plants of slaughterhouses is a rich source of chemical, organic, and microbiological constituents that can be biotechnologically exploited. The purpose of this study was to (i) conduct a chemical analysis of the sludge, and (ii) isolate, quantify, and describe the amylolytic, cellulolytic, ligninolytic, proteolytic, and keratinolytic bacteria in the sludge. Sludge samples were collected at the wastewater treatment plant of the Francap SA poultry company. The nutrient contents, C/N ratio, and pH were determined. For the bacterial count, 10 g sludge was diluted in 90 mL saline solution, which was serially diluted to 10-12. Aliquots of 100 µL of each dilution were transferred to selective media for isolation of bacteria that degrade organic substances. The colony-forming units were determined for each culture medium. Individual colonies were purified and characterized morphologically. The sludge contained 9.5, 1.21, and 0.45 dag kg-1 of N, P, and K, respectively. Fifty-two isolates were purified and characterized, with 2.11 × 1012 to 9.55 ×1015 colony-forming units per g sludge. In conclusion, the sludge generated in poultry slaughterhouse wastewater treatment plants is a rich source of organo-mineral constituents and bacteria with biotechnological potential for degrading organic substances.
first_indexed 2024-04-12T20:04:18Z
format Article
id doaj.art-258b92fed8674c6da1393df9f77a9884
institution Directory Open Access Journal
issn 1676-546X
1679-0359
language English
last_indexed 2024-04-12T20:04:18Z
publishDate 2017-06-01
publisher Universidade Estadual de Londrina
record_format Article
series Semina: Ciências Agrárias
spelling doaj.art-258b92fed8674c6da1393df9f77a98842022-12-22T03:18:27ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592017-06-013831209121610.5433/1679-0359.2017v38n3p120915313Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substancesJorge Avelino Rodriguez Lozada0Klever Cristiano Silveira1Libério Junio da Silva2Marihus Altoé Baldotto3Lílian Estrela Borges Baldotto4Universidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaThe sludge produced in wastewater treatment plants of slaughterhouses is a rich source of chemical, organic, and microbiological constituents that can be biotechnologically exploited. The purpose of this study was to (i) conduct a chemical analysis of the sludge, and (ii) isolate, quantify, and describe the amylolytic, cellulolytic, ligninolytic, proteolytic, and keratinolytic bacteria in the sludge. Sludge samples were collected at the wastewater treatment plant of the Francap SA poultry company. The nutrient contents, C/N ratio, and pH were determined. For the bacterial count, 10 g sludge was diluted in 90 mL saline solution, which was serially diluted to 10-12. Aliquots of 100 µL of each dilution were transferred to selective media for isolation of bacteria that degrade organic substances. The colony-forming units were determined for each culture medium. Individual colonies were purified and characterized morphologically. The sludge contained 9.5, 1.21, and 0.45 dag kg-1 of N, P, and K, respectively. Fifty-two isolates were purified and characterized, with 2.11 × 1012 to 9.55 ×1015 colony-forming units per g sludge. In conclusion, the sludge generated in poultry slaughterhouse wastewater treatment plants is a rich source of organo-mineral constituents and bacteria with biotechnological potential for degrading organic substances.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/25953Agricultural microbiologyOrganic matterSolid waste.
spellingShingle Jorge Avelino Rodriguez Lozada
Klever Cristiano Silveira
Libério Junio da Silva
Marihus Altoé Baldotto
Lílian Estrela Borges Baldotto
Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
Semina: Ciências Agrárias
Agricultural microbiology
Organic matter
Solid waste.
title Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
title_full Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
title_fullStr Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
title_full_unstemmed Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
title_short Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
title_sort prospecting for sludge bacteria from a poultry slaughterhouse with potential for degrading organic substances
topic Agricultural microbiology
Organic matter
Solid waste.
url http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/25953
work_keys_str_mv AT jorgeavelinorodriguezlozada prospectingforsludgebacteriafromapoultryslaughterhousewithpotentialfordegradingorganicsubstances
AT klevercristianosilveira prospectingforsludgebacteriafromapoultryslaughterhousewithpotentialfordegradingorganicsubstances
AT liberiojuniodasilva prospectingforsludgebacteriafromapoultryslaughterhousewithpotentialfordegradingorganicsubstances
AT marihusaltoebaldotto prospectingforsludgebacteriafromapoultryslaughterhousewithpotentialfordegradingorganicsubstances
AT lilianestrelaborgesbaldotto prospectingforsludgebacteriafromapoultryslaughterhousewithpotentialfordegradingorganicsubstances