Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitness
In sandflies, the absence of the peritrophic matrix (PM) affects the rate of blood digestion. Also, the kinetics of PM secretion varies according to species. We previously characterised PpChit1, a midgut-specific chitinase secreted in Phlebotomus papatasi (PPIS) that is involved in the maturation of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2014-12-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000801064&lng=en&tlng=en |
| _version_ | 1827844832510345216 |
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| author | Maricela Robles-Murguia Nicholas Bloedow Leigh Murray Marcelo Ramalho-Ortigão |
| author_facet | Maricela Robles-Murguia Nicholas Bloedow Leigh Murray Marcelo Ramalho-Ortigão |
| author_sort | Maricela Robles-Murguia |
| collection | DOAJ |
| description | In sandflies, the absence of the peritrophic matrix (PM) affects the rate of blood digestion. Also, the kinetics of PM secretion varies according to species. We previously characterised PpChit1, a midgut-specific chitinase secreted in Phlebotomus papatasi (PPIS) that is involved in the maturation of the PM and showed that antibodies against PpChit1 reduce the chitinolytic activity in the midgut of several sandfly species. Here, sandflies were fed on red blood cells reconstituted with naïve or anti-PpChit1 sera and assessed for fitness parameters that included blood digestion, oviposition onset, number of eggs laid, egg bouts, average number of eggs per bout and survival. In PPIS, anti-PpChit1 led to a one-day delay in the onset of egg laying, with flies surviving three days longer compared to the control group. Anti-PpChit1 also had a negative effect on overall ability of flies to lay eggs, as several gravid females from all three species were unable to lay any eggs despite having lived longer than control flies. Whereas the longer survival might be associated with improved haeme scavenging ability by the PM, the inability of females to lay eggs is possibly linked to changes in PM permeability affecting nutrient absorption. |
| first_indexed | 2024-03-12T08:48:32Z |
| format | Article |
| id | doaj.art-258f1cb1b5364a038f75f4ac68d4e5ea |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:48:32Z |
| publishDate | 2014-12-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-258f1cb1b5364a038f75f4ac68d4e5ea2023-09-02T16:22:39ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602014-12-0110981064106910.1590/0074-0276140382S0074-02762014000801064Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitnessMaricela Robles-MurguiaNicholas BloedowLeigh MurrayMarcelo Ramalho-OrtigãoIn sandflies, the absence of the peritrophic matrix (PM) affects the rate of blood digestion. Also, the kinetics of PM secretion varies according to species. We previously characterised PpChit1, a midgut-specific chitinase secreted in Phlebotomus papatasi (PPIS) that is involved in the maturation of the PM and showed that antibodies against PpChit1 reduce the chitinolytic activity in the midgut of several sandfly species. Here, sandflies were fed on red blood cells reconstituted with naïve or anti-PpChit1 sera and assessed for fitness parameters that included blood digestion, oviposition onset, number of eggs laid, egg bouts, average number of eggs per bout and survival. In PPIS, anti-PpChit1 led to a one-day delay in the onset of egg laying, with flies surviving three days longer compared to the control group. Anti-PpChit1 also had a negative effect on overall ability of flies to lay eggs, as several gravid females from all three species were unable to lay any eggs despite having lived longer than control flies. Whereas the longer survival might be associated with improved haeme scavenging ability by the PM, the inability of females to lay eggs is possibly linked to changes in PM permeability affecting nutrient absorption.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000801064&lng=en&tlng=ensandfliesPpChit1peritrophic matrixblood digestion |
| spellingShingle | Maricela Robles-Murguia Nicholas Bloedow Leigh Murray Marcelo Ramalho-Ortigão Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitness Memorias do Instituto Oswaldo Cruz sandflies PpChit1 peritrophic matrix blood digestion |
| title | Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitness |
| title_full | Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitness |
| title_fullStr | Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitness |
| title_full_unstemmed | Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitness |
| title_short | Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitness |
| title_sort | effect of mouse antisera targeting the phlebotomus papatasi midgut chitinase ppchit1 on sandfly physiology and fitness |
| topic | sandflies PpChit1 peritrophic matrix blood digestion |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000801064&lng=en&tlng=en |
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