Differentially Expressed Gene Patterns in Ascarid-Infected Chickens of Higher- or Lower-Performing Genotypes
Here, we describe the first transcriptomic investigation of the peripheral blood of chickens exposed to <i>Ascaridia galli</i> and <i>Heterakis gallinarum</i> infections. We investigated differentially expressed gene (DEG) patterns in two chicken genotypes with either a highe...
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MDPI AG
2021-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/4/1002 |
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| author | Isabel Kilic Manuel Stehr Mark Hennies Cornelia C. Metges Sören Franzenburg Clemens Falker-Gieske Jens Tetens Gürbüz Daş |
| author_facet | Isabel Kilic Manuel Stehr Mark Hennies Cornelia C. Metges Sören Franzenburg Clemens Falker-Gieske Jens Tetens Gürbüz Daş |
| author_sort | Isabel Kilic |
| collection | DOAJ |
| description | Here, we describe the first transcriptomic investigation of the peripheral blood of chickens exposed to <i>Ascaridia galli</i> and <i>Heterakis gallinarum</i> infections. We investigated differentially expressed gene (DEG) patterns in two chicken genotypes with either a higher (Lohmann Brown Plus, LB) or lower (Lohmann Dual, LD) laying performance level. The hens were experimentally coinfected with <i>A. galli</i> and <i>H. gallinarum</i>, and their worm burdens and infection parameters were determined six weeks post infection. Based on most representative infection parameters, the hens were clustered into lower- and higher-infection intensity classes. We identified a total of 78 DEGs contributing to infection-related phenotypic variation in the two genotypes. Our data showed significant upregulation of <i>Guanylate Binding Protein 7</i> (<i>GBP7</i>) in LD hens, making it a promising candidate for tolerance to ascarid infections in chickens. Gene ontology analysis revealed higher transcriptome activity related to biological processes such as “response to external stimulus” in LB hens, implying a higher stress response in this genotype. In contrast, LD hens showed higher transcriptomic expression of genes related to ontology classes that are possibly associated with a higher tolerance to infections. These findings may help explain why lower-performing genotypes (i.e., LD) are less sensitive to infections in terms of maintaining their performance. |
| first_indexed | 2024-03-10T12:38:24Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T12:38:24Z |
| publishDate | 2021-04-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-25905989d7a049538037589adeeb41d62023-11-21T14:03:55ZengMDPI AGAnimals2076-26152021-04-01114100210.3390/ani11041002Differentially Expressed Gene Patterns in Ascarid-Infected Chickens of Higher- or Lower-Performing GenotypesIsabel Kilic0Manuel Stehr1Mark Hennies2Cornelia C. Metges3Sören Franzenburg4Clemens Falker-Gieske5Jens Tetens6Gürbüz Daş7Department of Animal Sciences, University of Göttingen, 37077 Göttingen, GermanyInstitute of Nutritional Physiology ‘Oskar Kellner’, Leibniz Institute for Farm Animal Biology, 18196 Dummerstorf, GermanyTECOdevelopment GmbH, Marie-Curie-Str. 1, 53359 Rheinbach, GermanyInstitute of Nutritional Physiology ‘Oskar Kellner’, Leibniz Institute for Farm Animal Biology, 18196 Dummerstorf, GermanyInstitute of Clinical Molecular Biology, Christian-Albrechts-University of Kiel, 24105 Kiel, GermanyDepartment of Animal Sciences, University of Göttingen, 37077 Göttingen, GermanyDepartment of Animal Sciences, University of Göttingen, 37077 Göttingen, GermanyInstitute of Nutritional Physiology ‘Oskar Kellner’, Leibniz Institute for Farm Animal Biology, 18196 Dummerstorf, GermanyHere, we describe the first transcriptomic investigation of the peripheral blood of chickens exposed to <i>Ascaridia galli</i> and <i>Heterakis gallinarum</i> infections. We investigated differentially expressed gene (DEG) patterns in two chicken genotypes with either a higher (Lohmann Brown Plus, LB) or lower (Lohmann Dual, LD) laying performance level. The hens were experimentally coinfected with <i>A. galli</i> and <i>H. gallinarum</i>, and their worm burdens and infection parameters were determined six weeks post infection. Based on most representative infection parameters, the hens were clustered into lower- and higher-infection intensity classes. We identified a total of 78 DEGs contributing to infection-related phenotypic variation in the two genotypes. Our data showed significant upregulation of <i>Guanylate Binding Protein 7</i> (<i>GBP7</i>) in LD hens, making it a promising candidate for tolerance to ascarid infections in chickens. Gene ontology analysis revealed higher transcriptome activity related to biological processes such as “response to external stimulus” in LB hens, implying a higher stress response in this genotype. In contrast, LD hens showed higher transcriptomic expression of genes related to ontology classes that are possibly associated with a higher tolerance to infections. These findings may help explain why lower-performing genotypes (i.e., LD) are less sensitive to infections in terms of maintaining their performance.https://www.mdpi.com/2076-2615/11/4/1002host-parasite interactionhost performancenematode infectionperipheral bloodtolerancetranscriptome |
| spellingShingle | Isabel Kilic Manuel Stehr Mark Hennies Cornelia C. Metges Sören Franzenburg Clemens Falker-Gieske Jens Tetens Gürbüz Daş Differentially Expressed Gene Patterns in Ascarid-Infected Chickens of Higher- or Lower-Performing Genotypes Animals host-parasite interaction host performance nematode infection peripheral blood tolerance transcriptome |
| title | Differentially Expressed Gene Patterns in Ascarid-Infected Chickens of Higher- or Lower-Performing Genotypes |
| title_full | Differentially Expressed Gene Patterns in Ascarid-Infected Chickens of Higher- or Lower-Performing Genotypes |
| title_fullStr | Differentially Expressed Gene Patterns in Ascarid-Infected Chickens of Higher- or Lower-Performing Genotypes |
| title_full_unstemmed | Differentially Expressed Gene Patterns in Ascarid-Infected Chickens of Higher- or Lower-Performing Genotypes |
| title_short | Differentially Expressed Gene Patterns in Ascarid-Infected Chickens of Higher- or Lower-Performing Genotypes |
| title_sort | differentially expressed gene patterns in ascarid infected chickens of higher or lower performing genotypes |
| topic | host-parasite interaction host performance nematode infection peripheral blood tolerance transcriptome |
| url | https://www.mdpi.com/2076-2615/11/4/1002 |
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