Analysis of Human Papillomavirus (HPV) and Polyomaviruses (HPyVs) in Adenoid Cystic Carcinoma (AdCC) of the Head and Neck Region Reveals Three HPV-Positive Cases with Adenoid Cystic-like Features
An aetiological role of human papillomavirus (HPV) and/or human polyomaviruses (HPyVs) has been proposed in adenoid cystic carcinoma (AdCC). Moreover, HPV-related multiphenotypic carcinoma (HMSC) was recently introduced as an emerging entity of the sinonasal region. Here, we primarily want to study...
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2022-05-01
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| author | Mark Zupancic Stefan Holzhauser Liquin Cheng Torbjörn Ramqvist Juan Du Signe Friesland Anders Näsman Tina Dalianis |
| author_facet | Mark Zupancic Stefan Holzhauser Liquin Cheng Torbjörn Ramqvist Juan Du Signe Friesland Anders Näsman Tina Dalianis |
| author_sort | Mark Zupancic |
| collection | DOAJ |
| description | An aetiological role of human papillomavirus (HPV) and/or human polyomaviruses (HPyVs) has been proposed in adenoid cystic carcinoma (AdCC). Moreover, HPV-related multiphenotypic carcinoma (HMSC) was recently introduced as an emerging entity of the sinonasal region. Here, we primarily want to study the role of HPV/HPyV in a large AdCC cohort and, secondly, possibly identify and characterize HMSC. Tumour DNA from 68 patients initially diagnosed with AdCC between 2000 and 2012 was, therefore, tested for 27 HPV types and 10 HPyVs. HPV DNA-positive samples were micromorphologically re-evaluated, further stained for p16<sup>INK4a</sup>, S100, p63 and CD117 and tested for the presence of the MYB-NFIB fusion transcript. Notably, no samples were HPyV-positive, while one sinonasal and two tonsillar carcinomas were HPV- and p16-positive. After re-evaluating the micromorphology, immunohistochemistry and presence of fusion transcripts, all tumours had the same appearance and fitted within the diagnosis of HMSC, but in all these three cases, the morphology of the HMSC and basaloid squamous cell carcinoma was overlapping. We conclude that HPV and HPyV have no major role in AdCC. However, based on our data, we also suggest that HMSC should be considered as a basaloid variant of squamous cell carcinoma, and not its own entity, until better characterized. |
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| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T01:36:13Z |
| publishDate | 2022-05-01 |
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| series | Viruses |
| spelling | doaj.art-2590e1eaba7749349a310a076c1fa7d42023-11-23T13:32:16ZengMDPI AGViruses1999-49152022-05-01145104010.3390/v14051040Analysis of Human Papillomavirus (HPV) and Polyomaviruses (HPyVs) in Adenoid Cystic Carcinoma (AdCC) of the Head and Neck Region Reveals Three HPV-Positive Cases with Adenoid Cystic-like FeaturesMark Zupancic0Stefan Holzhauser1Liquin Cheng2Torbjörn Ramqvist3Juan Du4Signe Friesland5Anders Näsman6Tina Dalianis7Department of Oncology-Pathology, Karolinska Institutet, 17164 Stockholm, SwedenDepartment of Oncology-Pathology, Karolinska Institutet, 17164 Stockholm, SwedenDepartment of Microbiology, Tumour Biology and Cell Biology, Karolinska Institutet, 17177 Stockholm, SwedenDepartment of Oncology-Pathology, Karolinska Institutet, 17164 Stockholm, SwedenDepartment of Microbiology, Tumour Biology and Cell Biology, Karolinska Institutet, 17177 Stockholm, SwedenDepartment of Oncology-Pathology, Karolinska Institutet, 17164 Stockholm, SwedenDepartment of Oncology-Pathology, Karolinska Institutet, 17164 Stockholm, SwedenDepartment of Oncology-Pathology, Karolinska Institutet, 17164 Stockholm, SwedenAn aetiological role of human papillomavirus (HPV) and/or human polyomaviruses (HPyVs) has been proposed in adenoid cystic carcinoma (AdCC). Moreover, HPV-related multiphenotypic carcinoma (HMSC) was recently introduced as an emerging entity of the sinonasal region. Here, we primarily want to study the role of HPV/HPyV in a large AdCC cohort and, secondly, possibly identify and characterize HMSC. Tumour DNA from 68 patients initially diagnosed with AdCC between 2000 and 2012 was, therefore, tested for 27 HPV types and 10 HPyVs. HPV DNA-positive samples were micromorphologically re-evaluated, further stained for p16<sup>INK4a</sup>, S100, p63 and CD117 and tested for the presence of the MYB-NFIB fusion transcript. Notably, no samples were HPyV-positive, while one sinonasal and two tonsillar carcinomas were HPV- and p16-positive. After re-evaluating the micromorphology, immunohistochemistry and presence of fusion transcripts, all tumours had the same appearance and fitted within the diagnosis of HMSC, but in all these three cases, the morphology of the HMSC and basaloid squamous cell carcinoma was overlapping. We conclude that HPV and HPyV have no major role in AdCC. However, based on our data, we also suggest that HMSC should be considered as a basaloid variant of squamous cell carcinoma, and not its own entity, until better characterized.https://www.mdpi.com/1999-4915/14/5/1040adenoid cystic carcinomabasaloid carcinomahistopathologyHPV-related multiphenotypic sinonasal carcinomahuman papillomavirushuman papillomavirus carcinoma with adenoid cystic-like features |
| spellingShingle | Mark Zupancic Stefan Holzhauser Liquin Cheng Torbjörn Ramqvist Juan Du Signe Friesland Anders Näsman Tina Dalianis Analysis of Human Papillomavirus (HPV) and Polyomaviruses (HPyVs) in Adenoid Cystic Carcinoma (AdCC) of the Head and Neck Region Reveals Three HPV-Positive Cases with Adenoid Cystic-like Features Viruses adenoid cystic carcinoma basaloid carcinoma histopathology HPV-related multiphenotypic sinonasal carcinoma human papillomavirus human papillomavirus carcinoma with adenoid cystic-like features |
| title | Analysis of Human Papillomavirus (HPV) and Polyomaviruses (HPyVs) in Adenoid Cystic Carcinoma (AdCC) of the Head and Neck Region Reveals Three HPV-Positive Cases with Adenoid Cystic-like Features |
| title_full | Analysis of Human Papillomavirus (HPV) and Polyomaviruses (HPyVs) in Adenoid Cystic Carcinoma (AdCC) of the Head and Neck Region Reveals Three HPV-Positive Cases with Adenoid Cystic-like Features |
| title_fullStr | Analysis of Human Papillomavirus (HPV) and Polyomaviruses (HPyVs) in Adenoid Cystic Carcinoma (AdCC) of the Head and Neck Region Reveals Three HPV-Positive Cases with Adenoid Cystic-like Features |
| title_full_unstemmed | Analysis of Human Papillomavirus (HPV) and Polyomaviruses (HPyVs) in Adenoid Cystic Carcinoma (AdCC) of the Head and Neck Region Reveals Three HPV-Positive Cases with Adenoid Cystic-like Features |
| title_short | Analysis of Human Papillomavirus (HPV) and Polyomaviruses (HPyVs) in Adenoid Cystic Carcinoma (AdCC) of the Head and Neck Region Reveals Three HPV-Positive Cases with Adenoid Cystic-like Features |
| title_sort | analysis of human papillomavirus hpv and polyomaviruses hpyvs in adenoid cystic carcinoma adcc of the head and neck region reveals three hpv positive cases with adenoid cystic like features |
| topic | adenoid cystic carcinoma basaloid carcinoma histopathology HPV-related multiphenotypic sinonasal carcinoma human papillomavirus human papillomavirus carcinoma with adenoid cystic-like features |
| url | https://www.mdpi.com/1999-4915/14/5/1040 |
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