Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation
Although the clinical application of cell-free tissue-engineered vascular grafts (TEVGs) has been proposed, vascular tissue regeneration mechanisms have not been fully clarified. Here, we report that monocyte subpopulations reconstruct vascular-like tissues through integrin signaling. An Arg-Glu-Asp...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423003071 |
| _version_ | 1797646378082500608 |
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| author | Atsushi Mahara Manabu Shirai Raghav Soni Hue Thi Le Kaito Shimizu Yoshiaki Hirano Tetsuji Yamaoka |
| author_facet | Atsushi Mahara Manabu Shirai Raghav Soni Hue Thi Le Kaito Shimizu Yoshiaki Hirano Tetsuji Yamaoka |
| author_sort | Atsushi Mahara |
| collection | DOAJ |
| description | Although the clinical application of cell-free tissue-engineered vascular grafts (TEVGs) has been proposed, vascular tissue regeneration mechanisms have not been fully clarified. Here, we report that monocyte subpopulations reconstruct vascular-like tissues through integrin signaling. An Arg-Glu-Asp-Val peptide-modified acellular long-bypass graft was used as the TEVG, and tissue regeneration in the graft was evaluated using a cardiopulmonary pump system and porcine transplantation model. In 1 day, the luminal surface of the graft was covered with cells that expressed CD163, CD14, and CD16, which represented the monocyte subpopulation, and they exhibited proliferative and migratory abilities. RNA sequencing showed that captured cells had an immune-related phenotype similar to that of monocytes and strongly expressed cell adhesion-related genes. In vitro angiogenesis assay showed that tube formation of the captured cells occurred via integrin signal activation. After medium- and long-term graft transplantation, the captured cells infiltrated the tunica media layer and constructed vascular with a CD31/CD105-positive layer and an αSMA-positive structure after 3 months. This finding, including multiple early-time observations provides clear evidence that blood-circulating monocytes are directly involved in vascular remodeling. |
| first_indexed | 2024-03-11T15:01:31Z |
| format | Article |
| id | doaj.art-2595b36a298d4bdb80ccc2b6be259c99 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-11T15:01:31Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-2595b36a298d4bdb80ccc2b6be259c992023-10-30T06:08:36ZengElsevierMaterials Today Bio2590-00642023-12-0123100847Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activationAtsushi Mahara0Manabu Shirai1Raghav Soni2Hue Thi Le3Kaito Shimizu4Yoshiaki Hirano5Tetsuji Yamaoka6Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Kishibe Shimmachi, Suita Osaka, 564-8565, Japan; Corresponding author.Omics Research Center, National Cerebral and Cardiovascular Center Research Institute, Kishibe Shimmachi, Suita Osaka, 564-8565, JapanDepartment of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Kishibe Shimmachi, Suita Osaka, 564-8565, JapanDepartment of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Kishibe Shimmachi, Suita Osaka, 564-8565, JapanFaculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamatecho, Suita, Osaka, 565-8680, JapanFaculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamatecho, Suita, Osaka, 565-8680, JapanDepartment of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Kishibe Shimmachi, Suita Osaka, 564-8565, Japan; Corresponding author.Although the clinical application of cell-free tissue-engineered vascular grafts (TEVGs) has been proposed, vascular tissue regeneration mechanisms have not been fully clarified. Here, we report that monocyte subpopulations reconstruct vascular-like tissues through integrin signaling. An Arg-Glu-Asp-Val peptide-modified acellular long-bypass graft was used as the TEVG, and tissue regeneration in the graft was evaluated using a cardiopulmonary pump system and porcine transplantation model. In 1 day, the luminal surface of the graft was covered with cells that expressed CD163, CD14, and CD16, which represented the monocyte subpopulation, and they exhibited proliferative and migratory abilities. RNA sequencing showed that captured cells had an immune-related phenotype similar to that of monocytes and strongly expressed cell adhesion-related genes. In vitro angiogenesis assay showed that tube formation of the captured cells occurred via integrin signal activation. After medium- and long-term graft transplantation, the captured cells infiltrated the tunica media layer and constructed vascular with a CD31/CD105-positive layer and an αSMA-positive structure after 3 months. This finding, including multiple early-time observations provides clear evidence that blood-circulating monocytes are directly involved in vascular remodeling.http://www.sciencedirect.com/science/article/pii/S2590006423003071Tissue engineered vascular graftMonocyteIntegrinRegeneration |
| spellingShingle | Atsushi Mahara Manabu Shirai Raghav Soni Hue Thi Le Kaito Shimizu Yoshiaki Hirano Tetsuji Yamaoka Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation Materials Today Bio Tissue engineered vascular graft Monocyte Integrin Regeneration |
| title | Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation |
| title_full | Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation |
| title_fullStr | Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation |
| title_full_unstemmed | Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation |
| title_short | Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation |
| title_sort | vascular tissue reconstruction by monocyte subpopulations on small diameter acellular grafts via integrin activation |
| topic | Tissue engineered vascular graft Monocyte Integrin Regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423003071 |
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