Chromatographic Analysis of Aflatoxigenic <i>Aspergillus flavus</i> Isolated from Malaysian Sweet Corn
High-performance liquid chromatography (HPLC) provides a quick and efficient tool for accurately characterizing aflatoxigenic and non-aflatoxigenic isolates of <i>Aspergillus flavus</i>. This method also provides a quantitative analysis of AFs in <i>Aspergillus flavus.</i> Th...
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MDPI AG
2021-07-01
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| author | Rahim Khan Farinazleen Mohamad Ghazali Nor Ainy Mahyudin Nik Iskandar Putra Samsudin |
| author_facet | Rahim Khan Farinazleen Mohamad Ghazali Nor Ainy Mahyudin Nik Iskandar Putra Samsudin |
| author_sort | Rahim Khan |
| collection | DOAJ |
| description | High-performance liquid chromatography (HPLC) provides a quick and efficient tool for accurately characterizing aflatoxigenic and non-aflatoxigenic isolates of <i>Aspergillus flavus</i>. This method also provides a quantitative analysis of AFs in <i>Aspergillus flavus.</i> The method’s recovery was assessed by spiking a mixture of AF at different concentrations to the testing medium. The validity of the method was confirmed using aflatoxigenic and non-aflatoxigenic strains of <i>A. flavus</i>. The HPLC system, coupled with a fluorescence detector and post-column photochemical reactor, showed high sensitivity in detecting spiked AFs or AFs produced by <i>A. flavus</i> isolates. Recovery from medium spiked with 10, 20, 60, and 80 ppb of AFs was found to be 73–86% using this approach. For AFB<sub>1</sub> and AFB<sub>2</sub>, the limit of detection was 0.072 and 0.062 ppb, while the limit of quantification was 0.220 and 0.189 ppb, respectively. The AFB<sub>1</sub> concentrations ranged from 0.09 to 50.68 ppb, while the AFB<sub>2</sub> concentrations ranged between 0.33 and 9.23 ppb. The findings showed that six isolates produced more AFB<sub>1</sub> and AFB<sub>2</sub> than the acceptable limit of 5 ppb. The incidence of aflatoxigenic isolates of <i>A. flavus</i> in sweet corn and higher concentrations of AFB<sub>1</sub> and AFB<sub>2</sub> emphasize the need for field trials to explore their real potential for AF production in corn. |
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| spelling | doaj.art-2596359cf78c46caa8c68e1bade05eb72023-11-22T04:58:29ZengMDPI AGSeparations2297-87392021-07-01879810.3390/separations8070098Chromatographic Analysis of Aflatoxigenic <i>Aspergillus flavus</i> Isolated from Malaysian Sweet CornRahim Khan0Farinazleen Mohamad Ghazali1Nor Ainy Mahyudin2Nik Iskandar Putra Samsudin3Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, MalaysiaDepartment of Food Service and Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, MalaysiaHigh-performance liquid chromatography (HPLC) provides a quick and efficient tool for accurately characterizing aflatoxigenic and non-aflatoxigenic isolates of <i>Aspergillus flavus</i>. This method also provides a quantitative analysis of AFs in <i>Aspergillus flavus.</i> The method’s recovery was assessed by spiking a mixture of AF at different concentrations to the testing medium. The validity of the method was confirmed using aflatoxigenic and non-aflatoxigenic strains of <i>A. flavus</i>. The HPLC system, coupled with a fluorescence detector and post-column photochemical reactor, showed high sensitivity in detecting spiked AFs or AFs produced by <i>A. flavus</i> isolates. Recovery from medium spiked with 10, 20, 60, and 80 ppb of AFs was found to be 73–86% using this approach. For AFB<sub>1</sub> and AFB<sub>2</sub>, the limit of detection was 0.072 and 0.062 ppb, while the limit of quantification was 0.220 and 0.189 ppb, respectively. The AFB<sub>1</sub> concentrations ranged from 0.09 to 50.68 ppb, while the AFB<sub>2</sub> concentrations ranged between 0.33 and 9.23 ppb. The findings showed that six isolates produced more AFB<sub>1</sub> and AFB<sub>2</sub> than the acceptable limit of 5 ppb. The incidence of aflatoxigenic isolates of <i>A. flavus</i> in sweet corn and higher concentrations of AFB<sub>1</sub> and AFB<sub>2</sub> emphasize the need for field trials to explore their real potential for AF production in corn.https://www.mdpi.com/2297-8739/8/7/98<i>Aspergillus flavus</i>extractionHPLClimit of detectionlimit of quantitation |
| spellingShingle | Rahim Khan Farinazleen Mohamad Ghazali Nor Ainy Mahyudin Nik Iskandar Putra Samsudin Chromatographic Analysis of Aflatoxigenic <i>Aspergillus flavus</i> Isolated from Malaysian Sweet Corn Separations <i>Aspergillus flavus</i> extraction HPLC limit of detection limit of quantitation |
| title | Chromatographic Analysis of Aflatoxigenic <i>Aspergillus flavus</i> Isolated from Malaysian Sweet Corn |
| title_full | Chromatographic Analysis of Aflatoxigenic <i>Aspergillus flavus</i> Isolated from Malaysian Sweet Corn |
| title_fullStr | Chromatographic Analysis of Aflatoxigenic <i>Aspergillus flavus</i> Isolated from Malaysian Sweet Corn |
| title_full_unstemmed | Chromatographic Analysis of Aflatoxigenic <i>Aspergillus flavus</i> Isolated from Malaysian Sweet Corn |
| title_short | Chromatographic Analysis of Aflatoxigenic <i>Aspergillus flavus</i> Isolated from Malaysian Sweet Corn |
| title_sort | chromatographic analysis of aflatoxigenic i aspergillus flavus i isolated from malaysian sweet corn |
| topic | <i>Aspergillus flavus</i> extraction HPLC limit of detection limit of quantitation |
| url | https://www.mdpi.com/2297-8739/8/7/98 |
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