Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate
Taxol is a precious and effective anticancer drug. Cerium and methyl jasmonate (MJ) have been shown to increase the yield of taxol in taxus cells. However, the mechanisms of cerium-mediated and MJ-mediated taxol biosynthesis remain unknown. RNA-Seq was applied to study the overall regulation mechani...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-11-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1040596/full |
| _version_ | 1797988943214411776 |
|---|---|
| author | Na Han Wen-ji Geng Jian Li Shu-ting Liu Jie Zhang Yi-jie Wen Huai-hua Xu Meng-yuan Li Yan-ru Li Pei-pei Han |
| author_facet | Na Han Wen-ji Geng Jian Li Shu-ting Liu Jie Zhang Yi-jie Wen Huai-hua Xu Meng-yuan Li Yan-ru Li Pei-pei Han |
| author_sort | Na Han |
| collection | DOAJ |
| description | Taxol is a precious and effective anticancer drug. Cerium and methyl jasmonate (MJ) have been shown to increase the yield of taxol in taxus cells. However, the mechanisms of cerium-mediated and MJ-mediated taxol biosynthesis remain unknown. RNA-Seq was applied to study the overall regulation mechanism of cerium and MJ on taxol biosynthesis and analyze the differences among T. mairei cells elicited by Ce3+, Ce4+ and MJ on transcriptional level . Using sequence homology, 179 unigenes were identified as taxol synthesis genes. Under the condition of 100 μM MJ, taxol synthesis genes were up-regulated. Notably, taxol synthesis genes were down-regulated expression at 1 mM Ce3+ and 1 mM Ce4+. Differential expression genes involved in some related functions were analyzed, such as MAPK signaling pathway and plant-pathogen interaction. Sequence alignment and phylogenetic analysis of nine differentially expressed WRKYs in our data were carried out. |
| first_indexed | 2024-04-11T08:11:14Z |
| format | Article |
| id | doaj.art-25972346088144a4be41b309b157e141 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T08:11:14Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-25972346088144a4be41b309b157e1412022-12-22T04:35:19ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10405961040596Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonateNa HanWen-ji GengJian LiShu-ting LiuJie ZhangYi-jie WenHuai-hua XuMeng-yuan LiYan-ru LiPei-pei HanTaxol is a precious and effective anticancer drug. Cerium and methyl jasmonate (MJ) have been shown to increase the yield of taxol in taxus cells. However, the mechanisms of cerium-mediated and MJ-mediated taxol biosynthesis remain unknown. RNA-Seq was applied to study the overall regulation mechanism of cerium and MJ on taxol biosynthesis and analyze the differences among T. mairei cells elicited by Ce3+, Ce4+ and MJ on transcriptional level . Using sequence homology, 179 unigenes were identified as taxol synthesis genes. Under the condition of 100 μM MJ, taxol synthesis genes were up-regulated. Notably, taxol synthesis genes were down-regulated expression at 1 mM Ce3+ and 1 mM Ce4+. Differential expression genes involved in some related functions were analyzed, such as MAPK signaling pathway and plant-pathogen interaction. Sequence alignment and phylogenetic analysis of nine differentially expressed WRKYs in our data were carried out.https://www.frontiersin.org/articles/10.3389/fpls.2022.1040596/fullT. maireitranscriptometaxol biosynthesisceriumMJ |
| spellingShingle | Na Han Wen-ji Geng Jian Li Shu-ting Liu Jie Zhang Yi-jie Wen Huai-hua Xu Meng-yuan Li Yan-ru Li Pei-pei Han Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate Frontiers in Plant Science T. mairei transcriptome taxol biosynthesis cerium MJ |
| title | Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate |
| title_full | Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate |
| title_fullStr | Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate |
| title_full_unstemmed | Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate |
| title_short | Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate |
| title_sort | transcription level differences in taxus wallichiana var mairei elicited by ce3 ce4 and methyl jasmonate |
| topic | T. mairei transcriptome taxol biosynthesis cerium MJ |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1040596/full |
| work_keys_str_mv | AT nahan transcriptionleveldifferencesintaxuswallichianavarmaireielicitedbyce3ce4andmethyljasmonate AT wenjigeng transcriptionleveldifferencesintaxuswallichianavarmaireielicitedbyce3ce4andmethyljasmonate AT jianli transcriptionleveldifferencesintaxuswallichianavarmaireielicitedbyce3ce4andmethyljasmonate AT shutingliu transcriptionleveldifferencesintaxuswallichianavarmaireielicitedbyce3ce4andmethyljasmonate AT jiezhang transcriptionleveldifferencesintaxuswallichianavarmaireielicitedbyce3ce4andmethyljasmonate AT yijiewen transcriptionleveldifferencesintaxuswallichianavarmaireielicitedbyce3ce4andmethyljasmonate AT huaihuaxu transcriptionleveldifferencesintaxuswallichianavarmaireielicitedbyce3ce4andmethyljasmonate AT mengyuanli transcriptionleveldifferencesintaxuswallichianavarmaireielicitedbyce3ce4andmethyljasmonate AT yanruli transcriptionleveldifferencesintaxuswallichianavarmaireielicitedbyce3ce4andmethyljasmonate AT peipeihan transcriptionleveldifferencesintaxuswallichianavarmaireielicitedbyce3ce4andmethyljasmonate |