Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice
We previously showed that supplementation of a high fat diet with paramylon (PM) reduces the postprandial glucose rise, serum total and LDL cholesterol levels, and abdominal fat accumulation in mice. The purpose of this study was to explore the underlying mechanism of PM using microarray analysis. M...
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MDPI AG
2021-09-01
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| author | Seiichiro Aoe Chiemi Yamanaka Kento Mio |
| author_facet | Seiichiro Aoe Chiemi Yamanaka Kento Mio |
| author_sort | Seiichiro Aoe |
| collection | DOAJ |
| description | We previously showed that supplementation of a high fat diet with paramylon (PM) reduces the postprandial glucose rise, serum total and LDL cholesterol levels, and abdominal fat accumulation in mice. The purpose of this study was to explore the underlying mechanism of PM using microarray analysis. Male mice (C57BL/BL strain) were fed an experimental diet (50% fat energy) containing 5% PM isolated from <i>Euglena gracilis</i> EOD-1 for 12 weeks. After confirming that PM had an improving effect on lipid metabolism, we assessed ileal and hepatic mRNA expression using DNA microarray and subsequent analysis by gene ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The results suggested that dietary supplementation with PM resulted in decreased abdominal fat accumulation and serum LDL cholesterol concentrations via suppression of the digestion and absorption pathway in the ileum and activation of the hepatic PPAR signaling pathway. Postprandial glucose rise was reduced in mice fed PM, whereas changes in the glucose metabolism pathway were not detected in GO classification and KEGG pathway analysis. PM intake might enhance serum secretory immunoglobulin A concentrations via promotion of the immunoglobulin production pathway in the ileum. |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T06:17:58Z |
| publishDate | 2021-09-01 |
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| series | Nutrients |
| spelling | doaj.art-2598727078f24913b950fcd7658645b02023-11-22T19:28:05ZengMDPI AGNutrients2072-66432021-09-011310340610.3390/nu13103406Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese MiceSeiichiro Aoe0Chiemi Yamanaka1Kento Mio2The Institute of Human Culture Studies, Otsuma Women’s University, Chiyoda-ku, Tokyo 102-8357, JapanThe Institute of Human Culture Studies, Otsuma Women’s University, Chiyoda-ku, Tokyo 102-8357, JapanStudies in Human Life Sciences, Graduate School of Studies in Human Culture, Otsuma Women’s University, Chiyoda-ku, Tokyo 102-8357, JapanWe previously showed that supplementation of a high fat diet with paramylon (PM) reduces the postprandial glucose rise, serum total and LDL cholesterol levels, and abdominal fat accumulation in mice. The purpose of this study was to explore the underlying mechanism of PM using microarray analysis. Male mice (C57BL/BL strain) were fed an experimental diet (50% fat energy) containing 5% PM isolated from <i>Euglena gracilis</i> EOD-1 for 12 weeks. After confirming that PM had an improving effect on lipid metabolism, we assessed ileal and hepatic mRNA expression using DNA microarray and subsequent analysis by gene ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The results suggested that dietary supplementation with PM resulted in decreased abdominal fat accumulation and serum LDL cholesterol concentrations via suppression of the digestion and absorption pathway in the ileum and activation of the hepatic PPAR signaling pathway. Postprandial glucose rise was reduced in mice fed PM, whereas changes in the glucose metabolism pathway were not detected in GO classification and KEGG pathway analysis. PM intake might enhance serum secretory immunoglobulin A concentrations via promotion of the immunoglobulin production pathway in the ileum.https://www.mdpi.com/2072-6643/13/10/3406paramylonabdominal fatDNA microarraygene ontologyPPAR signaling |
| spellingShingle | Seiichiro Aoe Chiemi Yamanaka Kento Mio Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice Nutrients paramylon abdominal fat DNA microarray gene ontology PPAR signaling |
| title | Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice |
| title_full | Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice |
| title_fullStr | Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice |
| title_full_unstemmed | Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice |
| title_short | Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice |
| title_sort | microarray analysis of paramylon isolated from euglena gracilis eod 1 and its effects on lipid metabolism in the ileum and liver in diet induced obese mice |
| topic | paramylon abdominal fat DNA microarray gene ontology PPAR signaling |
| url | https://www.mdpi.com/2072-6643/13/10/3406 |
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