Transcriptional landscape of glomerular parietal epithelial cells.
Very little is known about the function of glomerular parietal epithelial cells (PECs). In this study, we performed genome-wide expression analysis on PEC-enriched capsulated vs. PEC-deprived decapsulated rat glomeruli to determine the transcriptional state of PECs under normal conditions. We identi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4134297?pdf=render |
| _version_ | 1818874221935198208 |
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| author | Sina A Gharib Jeffrey W Pippin Takamoto Ohse Scott G Pickering Ronald D Krofft Stuart J Shankland |
| author_facet | Sina A Gharib Jeffrey W Pippin Takamoto Ohse Scott G Pickering Ronald D Krofft Stuart J Shankland |
| author_sort | Sina A Gharib |
| collection | DOAJ |
| description | Very little is known about the function of glomerular parietal epithelial cells (PECs). In this study, we performed genome-wide expression analysis on PEC-enriched capsulated vs. PEC-deprived decapsulated rat glomeruli to determine the transcriptional state of PECs under normal conditions. We identified hundreds of differentially expressed genes that mapped to distinct biologic modules including development, tight junction, ion transport, and metabolic processes. Since developmental programs were highly enriched in PECs, we characterized several of their candidate members at the protein level. Collectively, our findings confirm that PECs are multifaceted cells and help define their diverse functional repertoire. |
| first_indexed | 2024-12-19T13:07:10Z |
| format | Article |
| id | doaj.art-2598d6e6621040d7b2451a55d8206edb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T13:07:10Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2598d6e6621040d7b2451a55d8206edb2022-12-21T20:20:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10528910.1371/journal.pone.0105289Transcriptional landscape of glomerular parietal epithelial cells.Sina A GharibJeffrey W PippinTakamoto OhseScott G PickeringRonald D KrofftStuart J ShanklandVery little is known about the function of glomerular parietal epithelial cells (PECs). In this study, we performed genome-wide expression analysis on PEC-enriched capsulated vs. PEC-deprived decapsulated rat glomeruli to determine the transcriptional state of PECs under normal conditions. We identified hundreds of differentially expressed genes that mapped to distinct biologic modules including development, tight junction, ion transport, and metabolic processes. Since developmental programs were highly enriched in PECs, we characterized several of their candidate members at the protein level. Collectively, our findings confirm that PECs are multifaceted cells and help define their diverse functional repertoire.http://europepmc.org/articles/PMC4134297?pdf=render |
| spellingShingle | Sina A Gharib Jeffrey W Pippin Takamoto Ohse Scott G Pickering Ronald D Krofft Stuart J Shankland Transcriptional landscape of glomerular parietal epithelial cells. PLoS ONE |
| title | Transcriptional landscape of glomerular parietal epithelial cells. |
| title_full | Transcriptional landscape of glomerular parietal epithelial cells. |
| title_fullStr | Transcriptional landscape of glomerular parietal epithelial cells. |
| title_full_unstemmed | Transcriptional landscape of glomerular parietal epithelial cells. |
| title_short | Transcriptional landscape of glomerular parietal epithelial cells. |
| title_sort | transcriptional landscape of glomerular parietal epithelial cells |
| url | http://europepmc.org/articles/PMC4134297?pdf=render |
| work_keys_str_mv | AT sinaagharib transcriptionallandscapeofglomerularparietalepithelialcells AT jeffreywpippin transcriptionallandscapeofglomerularparietalepithelialcells AT takamotoohse transcriptionallandscapeofglomerularparietalepithelialcells AT scottgpickering transcriptionallandscapeofglomerularparietalepithelialcells AT ronalddkrofft transcriptionallandscapeofglomerularparietalepithelialcells AT stuartjshankland transcriptionallandscapeofglomerularparietalepithelialcells |