Perfluorooctanoic Acid (PFOA) Exposure Compromises Fertility by Affecting Ovarian and Oocyte Development
PFOA, a newly emerging persistent organic pollutant, is widely present in various environmental media. Previous reports have proved that PFOA exposure can accumulate in the ovary and lead to reproductive toxicity in pregnant mice. However, the potential mechanism of PFOA exposure on fertility remain...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/136 |
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| author | Han Zhang Lulu Han Lijun Qiu Bo Zhao Yang Gao Zhangjie Chu Xiaoxin Dai |
| author_facet | Han Zhang Lulu Han Lijun Qiu Bo Zhao Yang Gao Zhangjie Chu Xiaoxin Dai |
| author_sort | Han Zhang |
| collection | DOAJ |
| description | PFOA, a newly emerging persistent organic pollutant, is widely present in various environmental media. Previous reports have proved that PFOA exposure can accumulate in the ovary and lead to reproductive toxicity in pregnant mice. However, the potential mechanism of PFOA exposure on fertility remains unclear. In this study, we explore how PFOA compromises fertility in the zebrafish. The data show that PFOA (100 mg/L for 15 days) exposure significantly impaired fertilization and hatching capability. Based on tissue sections, we found that PFOA exposure led to ovarian damage and a decrease in the percentage of mature oocytes. Moreover, through in vitro incubation, we determined that PFOA inhibits oocyte development. We also sequenced the transcriptome of the ovary of female zebrafish and a total of 284 overlapping DEGs were obtained. Functional enrichment analysis showed that 284 overlapping DEGs function mainly in complement and coagulation cascades signaling pathways. In addition, we identified genes that may be associated with immunity, such as LOC108191474 and ZGC:173837. We found that exposure to PFOA can cause an inflammatory response that can lead to ovarian damage and delayed oocyte development. |
| first_indexed | 2024-03-08T15:06:07Z |
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| id | doaj.art-259a28e0baa04e07a578975281176a10 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T15:06:07Z |
| publishDate | 2023-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-259a28e0baa04e07a578975281176a102024-01-10T14:58:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0125113610.3390/ijms25010136Perfluorooctanoic Acid (PFOA) Exposure Compromises Fertility by Affecting Ovarian and Oocyte DevelopmentHan Zhang0Lulu Han1Lijun Qiu2Bo Zhao3Yang Gao4Zhangjie Chu5Xiaoxin Dai6School of Fisheries, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Fisheries, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Fisheries, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Fisheries, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Fisheries, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Fisheries, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Fisheries, Zhejiang Ocean University, Zhoushan 316022, ChinaPFOA, a newly emerging persistent organic pollutant, is widely present in various environmental media. Previous reports have proved that PFOA exposure can accumulate in the ovary and lead to reproductive toxicity in pregnant mice. However, the potential mechanism of PFOA exposure on fertility remains unclear. In this study, we explore how PFOA compromises fertility in the zebrafish. The data show that PFOA (100 mg/L for 15 days) exposure significantly impaired fertilization and hatching capability. Based on tissue sections, we found that PFOA exposure led to ovarian damage and a decrease in the percentage of mature oocytes. Moreover, through in vitro incubation, we determined that PFOA inhibits oocyte development. We also sequenced the transcriptome of the ovary of female zebrafish and a total of 284 overlapping DEGs were obtained. Functional enrichment analysis showed that 284 overlapping DEGs function mainly in complement and coagulation cascades signaling pathways. In addition, we identified genes that may be associated with immunity, such as LOC108191474 and ZGC:173837. We found that exposure to PFOA can cause an inflammatory response that can lead to ovarian damage and delayed oocyte development.https://www.mdpi.com/1422-0067/25/1/136zebrafishfertilization capabilityoocytesperfluorooctanoic acid |
| spellingShingle | Han Zhang Lulu Han Lijun Qiu Bo Zhao Yang Gao Zhangjie Chu Xiaoxin Dai Perfluorooctanoic Acid (PFOA) Exposure Compromises Fertility by Affecting Ovarian and Oocyte Development International Journal of Molecular Sciences zebrafish fertilization capability oocytes perfluorooctanoic acid |
| title | Perfluorooctanoic Acid (PFOA) Exposure Compromises Fertility by Affecting Ovarian and Oocyte Development |
| title_full | Perfluorooctanoic Acid (PFOA) Exposure Compromises Fertility by Affecting Ovarian and Oocyte Development |
| title_fullStr | Perfluorooctanoic Acid (PFOA) Exposure Compromises Fertility by Affecting Ovarian and Oocyte Development |
| title_full_unstemmed | Perfluorooctanoic Acid (PFOA) Exposure Compromises Fertility by Affecting Ovarian and Oocyte Development |
| title_short | Perfluorooctanoic Acid (PFOA) Exposure Compromises Fertility by Affecting Ovarian and Oocyte Development |
| title_sort | perfluorooctanoic acid pfoa exposure compromises fertility by affecting ovarian and oocyte development |
| topic | zebrafish fertilization capability oocytes perfluorooctanoic acid |
| url | https://www.mdpi.com/1422-0067/25/1/136 |
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