Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates
This study aimed to assess the antibacterial and antibiotic potentiating property of Vangueria madagascariensis (VM) (fruit and leaf extracts) against 10 clinical isolates. A microdilution broth susceptibility assay for bacteria was used for the determination of the minimum inhibitory concentration...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-10-01
|
| Series: | Journal of Traditional and Complementary Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411015000929 |
| _version_ | 1818149026721169408 |
|---|---|
| author | M. Fawzi Mahomoodally Schajeed Dilmohamed |
| author_facet | M. Fawzi Mahomoodally Schajeed Dilmohamed |
| author_sort | M. Fawzi Mahomoodally |
| collection | DOAJ |
| description | This study aimed to assess the antibacterial and antibiotic potentiating property of Vangueria madagascariensis (VM) (fruit and leaf extracts) against 10 clinical isolates. A microdilution broth susceptibility assay for bacteria was used for the determination of the minimum inhibitory concentration (MIC) and associated with antibiotics to evaluate any synergistic effect. VM extracts were found to potentiate the activity of 3 conventional antibiotics. Chloramphenicol and Ciprofloxacin showed no activity against Acinetobacter spp. but when mixed with VM (in a ratio of 50% VM extracts: 30% antibiotic), showed potentiating effect. The methanolic fruit extract at lower concentration of Chloramphenicol (30%) gave better synergistic effect (MIC = 3.75 μg/mL) as compared to 50% (MIC = 12.5 μg/mL). With Gentamicin, no activity was detected with leaf decoction but other extracts (methanolic leaf/fruit extract and fruit decoction) showed enhancement (MIC- 0.47, 7.5 and 15 μg/mL respectively). Interestingly, Chloramphenicol showed no activity against MRSA, but when mixed with VM, produced low MICs (<0.39–0.78 μg/mL with 50% antibiotic and from <0.47 to 0.94 μg/mL with 30% antibiotic). Combining Gentamicin with VM extracts showed an enhancement in the potentiating activity against MRSA. In conclusion, the observed antimicrobial property of VM tend to suggest a promising alternative and complementary strategy to manage bacterial infections and hence can open new avenues for further research using traditional medicinal food plant. |
| first_indexed | 2024-12-11T13:00:30Z |
| format | Article |
| id | doaj.art-259b6511875a404e8b7bc19705343092 |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-12-11T13:00:30Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-259b6511875a404e8b7bc197053430922022-12-22T01:06:28ZengElsevierJournal of Traditional and Complementary Medicine2225-41102016-10-016439940310.1016/j.jtcme.2015.09.002Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolatesM. Fawzi Mahomoodally0Schajeed Dilmohamed1Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, MauritiusDepartment of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, MauritiusThis study aimed to assess the antibacterial and antibiotic potentiating property of Vangueria madagascariensis (VM) (fruit and leaf extracts) against 10 clinical isolates. A microdilution broth susceptibility assay for bacteria was used for the determination of the minimum inhibitory concentration (MIC) and associated with antibiotics to evaluate any synergistic effect. VM extracts were found to potentiate the activity of 3 conventional antibiotics. Chloramphenicol and Ciprofloxacin showed no activity against Acinetobacter spp. but when mixed with VM (in a ratio of 50% VM extracts: 30% antibiotic), showed potentiating effect. The methanolic fruit extract at lower concentration of Chloramphenicol (30%) gave better synergistic effect (MIC = 3.75 μg/mL) as compared to 50% (MIC = 12.5 μg/mL). With Gentamicin, no activity was detected with leaf decoction but other extracts (methanolic leaf/fruit extract and fruit decoction) showed enhancement (MIC- 0.47, 7.5 and 15 μg/mL respectively). Interestingly, Chloramphenicol showed no activity against MRSA, but when mixed with VM, produced low MICs (<0.39–0.78 μg/mL with 50% antibiotic and from <0.47 to 0.94 μg/mL with 30% antibiotic). Combining Gentamicin with VM extracts showed an enhancement in the potentiating activity against MRSA. In conclusion, the observed antimicrobial property of VM tend to suggest a promising alternative and complementary strategy to manage bacterial infections and hence can open new avenues for further research using traditional medicinal food plant.http://www.sciencedirect.com/science/article/pii/S2225411015000929Vangueria madagascariensisAntimicrobialsAntibiotic potentiatingFood plantMauritius |
| spellingShingle | M. Fawzi Mahomoodally Schajeed Dilmohamed Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates Journal of Traditional and Complementary Medicine Vangueria madagascariensis Antimicrobials Antibiotic potentiating Food plant Mauritius |
| title | Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
| title_full | Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
| title_fullStr | Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
| title_full_unstemmed | Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
| title_short | Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
| title_sort | antibacterial and antibiotic potentiating activity of vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
| topic | Vangueria madagascariensis Antimicrobials Antibiotic potentiating Food plant Mauritius |
| url | http://www.sciencedirect.com/science/article/pii/S2225411015000929 |
| work_keys_str_mv | AT mfawzimahomoodally antibacterialandantibioticpotentiatingactivityofvangueriamadagascariensisleavesandripefruitpericarpagainsthumanpathogenicclinicalbacterialisolates AT schajeeddilmohamed antibacterialandantibioticpotentiatingactivityofvangueriamadagascariensisleavesandripefruitpericarpagainsthumanpathogenicclinicalbacterialisolates |