Genetic variants in miR-145 gene are associated with the risk of asthma in Taiwan
Abstract Asthma is a chronic airway inflammation disease and the diagnosis and treatment strategies remain difficult. MicroRNAs play important roles in many biological and pathological processes including asthma development. There is no study confirming the contribution of genetic variants in miR-14...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-18587-w |
| _version_ | 1811211612889022464 |
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| author | Shou-Cheng Wang Chia-Wen Tsai Wen-Shin Chang Ning-Yi Hsia Mei-Chin Mong Yun-Chi Wang Te-Chun Hsia Jian Gu Da-Tian Bau |
| author_facet | Shou-Cheng Wang Chia-Wen Tsai Wen-Shin Chang Ning-Yi Hsia Mei-Chin Mong Yun-Chi Wang Te-Chun Hsia Jian Gu Da-Tian Bau |
| author_sort | Shou-Cheng Wang |
| collection | DOAJ |
| description | Abstract Asthma is a chronic airway inflammation disease and the diagnosis and treatment strategies remain difficult. MicroRNAs play important roles in many biological and pathological processes including asthma development. There is no study confirming the contribution of genetic variants in miR-145 to asthma etiology. We hypothesize that single nucleotide polymorphisms (SNPs) in the promoter region of miR-145 may be associated with the risk of asthma in Taiwanese. We used a case–control study to test this hypothesis. In 198 asthma patients and 453 healthy controls, the genotypes of miR-145 rs4705342 and rs4705343 were determined, and the associations of miR-145 genotypes with asthma risk and severity were evaluated. The distribution of miR-145 rs4705342 genotypes between asthma patients and non-asthmatic control groups were significantly different (p = 0.0187). In multivariable logistic regression analysis, compared with the wild-type TT genotype, individuals carrying the variant genotypes had progressively decreased risks of asthma: the odds ratio (OR) for the heterogeneous variant genotype (CT) and homozygous variant genotype (CC) was 0.77 (95% CI 0.55–1.10, p = 0.1788) and 0.41 (95% CI 0.21–0.79, p = 0.0102), respectively (p for trend = 0.0187). In allelic test, the C allele was associated with a 31% reduced risk of asthma (OR = 0.69, 95% CI 0.53–0.90, p = 0.0070). In addition, the rs4705342 variant genotypes were correlated with the symptom severity (p = 3 × 10–5). Furthermore, the variant genotypes correlated with lower miR-145-5p expression level in serum (p = 0.0001). As for rs4705343, there was no differential distribution of genotypes between cases and controls. Our data provide evidence for miR-145 rs4705342 to serve as a novel biomarker for asthma risk prediction. |
| first_indexed | 2024-04-12T05:15:38Z |
| format | Article |
| id | doaj.art-259c6859b27248d8b593535da2d66e70 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T05:15:38Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-259c6859b27248d8b593535da2d66e702022-12-22T03:46:37ZengNature PortfolioScientific Reports2045-23222022-09-011211910.1038/s41598-022-18587-wGenetic variants in miR-145 gene are associated with the risk of asthma in TaiwanShou-Cheng Wang0Chia-Wen Tsai1Wen-Shin Chang2Ning-Yi Hsia3Mei-Chin Mong4Yun-Chi Wang5Te-Chun Hsia6Jian Gu7Da-Tian Bau8Taichung Armed Forces General HospitalGraduate Institute of Biomedical Sciences, China Medical UniversityGraduate Institute of Biomedical Sciences, China Medical UniversityTerry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University HospitalDepartment of Food Nutrition and Health Biotechnology, Asia UniversityGraduate Institute of Biomedical Sciences, China Medical UniversityTerry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University HospitalDepartment of Epidemiology, The University of Texas MD Anderson Cancer CenterGraduate Institute of Biomedical Sciences, China Medical UniversityAbstract Asthma is a chronic airway inflammation disease and the diagnosis and treatment strategies remain difficult. MicroRNAs play important roles in many biological and pathological processes including asthma development. There is no study confirming the contribution of genetic variants in miR-145 to asthma etiology. We hypothesize that single nucleotide polymorphisms (SNPs) in the promoter region of miR-145 may be associated with the risk of asthma in Taiwanese. We used a case–control study to test this hypothesis. In 198 asthma patients and 453 healthy controls, the genotypes of miR-145 rs4705342 and rs4705343 were determined, and the associations of miR-145 genotypes with asthma risk and severity were evaluated. The distribution of miR-145 rs4705342 genotypes between asthma patients and non-asthmatic control groups were significantly different (p = 0.0187). In multivariable logistic regression analysis, compared with the wild-type TT genotype, individuals carrying the variant genotypes had progressively decreased risks of asthma: the odds ratio (OR) for the heterogeneous variant genotype (CT) and homozygous variant genotype (CC) was 0.77 (95% CI 0.55–1.10, p = 0.1788) and 0.41 (95% CI 0.21–0.79, p = 0.0102), respectively (p for trend = 0.0187). In allelic test, the C allele was associated with a 31% reduced risk of asthma (OR = 0.69, 95% CI 0.53–0.90, p = 0.0070). In addition, the rs4705342 variant genotypes were correlated with the symptom severity (p = 3 × 10–5). Furthermore, the variant genotypes correlated with lower miR-145-5p expression level in serum (p = 0.0001). As for rs4705343, there was no differential distribution of genotypes between cases and controls. Our data provide evidence for miR-145 rs4705342 to serve as a novel biomarker for asthma risk prediction.https://doi.org/10.1038/s41598-022-18587-w |
| spellingShingle | Shou-Cheng Wang Chia-Wen Tsai Wen-Shin Chang Ning-Yi Hsia Mei-Chin Mong Yun-Chi Wang Te-Chun Hsia Jian Gu Da-Tian Bau Genetic variants in miR-145 gene are associated with the risk of asthma in Taiwan Scientific Reports |
| title | Genetic variants in miR-145 gene are associated with the risk of asthma in Taiwan |
| title_full | Genetic variants in miR-145 gene are associated with the risk of asthma in Taiwan |
| title_fullStr | Genetic variants in miR-145 gene are associated with the risk of asthma in Taiwan |
| title_full_unstemmed | Genetic variants in miR-145 gene are associated with the risk of asthma in Taiwan |
| title_short | Genetic variants in miR-145 gene are associated with the risk of asthma in Taiwan |
| title_sort | genetic variants in mir 145 gene are associated with the risk of asthma in taiwan |
| url | https://doi.org/10.1038/s41598-022-18587-w |
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