WD40-domain protein GORI is an integrative scaffold that is required for pollen tube growth in rice
The pollen tube plays a critical role in angiosperm plants by delivering sperm gametes for double fertilization. Although the molecular mechanisms underlying pollen tube germination and growth are crucial to crop plants, they are poorly understood. Here, we describe recent advancements in the unders...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2022.2082678 |
| _version_ | 1827379154166743040 |
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| author | Yu-Jin Kim Ki-Hong Jung |
| author_facet | Yu-Jin Kim Ki-Hong Jung |
| author_sort | Yu-Jin Kim |
| collection | DOAJ |
| description | The pollen tube plays a critical role in angiosperm plants by delivering sperm gametes for double fertilization. Although the molecular mechanisms underlying pollen tube germination and growth are crucial to crop plants, they are poorly understood. Here, we describe recent advancements in the understanding of the role of the WD40-domain protein in regulating pollen germination and discuss future directions to investigate its role in rice. GORI encodes a seven-WD40-motif protein that interacts with an AP180 N-terminal homology (ANTH)-domain protein, which modulates clathrin-mediated endocytosis (CME), and regulates Rac6 activity in the apical plasma membrane of elongating pollen tubes. Loss of function of GORI or Rac6 reduces pollen germination and tube growth, thereby resulting in male sterility in rice. In contrast, overexpression of Rac6 increases pollen tube elongation, with this effect being rescued by GORI overexpression. In the absence of ANTH, pollen germination was reduced, similar to the results observed after inhibitor treatment, indicating that pollen germination partially requires CME. Our findings demonstrated that the GORI protein is a positive regulator of pollen germination and tube growth, serving as a link between Rac6 activity regulation and ANTH-mediated endocytosis. |
| first_indexed | 2024-03-08T13:08:03Z |
| format | Article |
| id | doaj.art-259e5f85c42c4a4c89c6b735268b7bc0 |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:08:03Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj.art-259e5f85c42c4a4c89c6b735268b7bc02024-01-18T15:58:21ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242023-12-0118110.1080/15592324.2022.20826782082678WD40-domain protein GORI is an integrative scaffold that is required for pollen tube growth in riceYu-Jin Kim0Ki-Hong Jung1Pusan National UniversityKyung Hee UniversityThe pollen tube plays a critical role in angiosperm plants by delivering sperm gametes for double fertilization. Although the molecular mechanisms underlying pollen tube germination and growth are crucial to crop plants, they are poorly understood. Here, we describe recent advancements in the understanding of the role of the WD40-domain protein in regulating pollen germination and discuss future directions to investigate its role in rice. GORI encodes a seven-WD40-motif protein that interacts with an AP180 N-terminal homology (ANTH)-domain protein, which modulates clathrin-mediated endocytosis (CME), and regulates Rac6 activity in the apical plasma membrane of elongating pollen tubes. Loss of function of GORI or Rac6 reduces pollen germination and tube growth, thereby resulting in male sterility in rice. In contrast, overexpression of Rac6 increases pollen tube elongation, with this effect being rescued by GORI overexpression. In the absence of ANTH, pollen germination was reduced, similar to the results observed after inhibitor treatment, indicating that pollen germination partially requires CME. Our findings demonstrated that the GORI protein is a positive regulator of pollen germination and tube growth, serving as a link between Rac6 activity regulation and ANTH-mediated endocytosis.http://dx.doi.org/10.1080/15592324.2022.2082678ricepollen tube growthwd40-domain proteinanthropendocytosis |
| spellingShingle | Yu-Jin Kim Ki-Hong Jung WD40-domain protein GORI is an integrative scaffold that is required for pollen tube growth in rice Plant Signaling & Behavior rice pollen tube growth wd40-domain protein anth rop endocytosis |
| title | WD40-domain protein GORI is an integrative scaffold that is required for pollen tube growth in rice |
| title_full | WD40-domain protein GORI is an integrative scaffold that is required for pollen tube growth in rice |
| title_fullStr | WD40-domain protein GORI is an integrative scaffold that is required for pollen tube growth in rice |
| title_full_unstemmed | WD40-domain protein GORI is an integrative scaffold that is required for pollen tube growth in rice |
| title_short | WD40-domain protein GORI is an integrative scaffold that is required for pollen tube growth in rice |
| title_sort | wd40 domain protein gori is an integrative scaffold that is required for pollen tube growth in rice |
| topic | rice pollen tube growth wd40-domain protein anth rop endocytosis |
| url | http://dx.doi.org/10.1080/15592324.2022.2082678 |
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