Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients
Diabetic cardiovascular diseases are leading causes of death. In diabetic patients, oxidative stress is key event in cardiovascular disease (CVD) development. Uncoupling protein 2 (UCP2) and myeloperoxidase (MPO) were identified as molecules regulating oxidative stress effects of oxygen free radical...
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| Format: | Article |
| Language: | English |
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Beni-Suef University, Faculty of Medicine
2023-01-01
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| Series: | Egyptian Journal of Medical Research |
| Subjects: | |
| Online Access: | https://ejmr.journals.ekb.eg/article_287657_d41d8cd98f00b204e9800998ecf8427e.pdf |
| _version_ | 1797893971969572864 |
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| author | Hanan Mohamed Farhan Khadiga Abo Gabl Iman Atef Mandour Heba Nabil Baz Neveen Ahmed Khalil Mahmoud Nassar Mohamed aly Gabr |
| author_facet | Hanan Mohamed Farhan Khadiga Abo Gabl Iman Atef Mandour Heba Nabil Baz Neveen Ahmed Khalil Mahmoud Nassar Mohamed aly Gabr |
| author_sort | Hanan Mohamed Farhan |
| collection | DOAJ |
| description | Diabetic cardiovascular diseases are leading causes of death. In diabetic patients, oxidative stress is key event in cardiovascular disease (CVD) development. Uncoupling protein 2 (UCP2) and myeloperoxidase (MPO) were identified as molecules regulating oxidative stress effects of oxygen free radicals. This work aimed to evaluate association of UCP2-866G/A (rs659366) gene polymorphism and serum MPO with CVD among type 2 diabetic patients. The present work was conducted on 80 type 2 diabetic Egyptian patients including 40 patients with CVD compared to 40 age- and sex-matched non-CVD patients; recruited from Outpatient Clinics in Beni-suef University Hospital and Kasr Eleiny Hospital. Lipid profile was assayed. Serum MPO was analyzed using enzyme-linked immunosorbent assay. UCP2-866G/A (rs659366) gene polymorphism was determined using polymerase chain reaction-restriction fragment length polymorphism. Statistically significant increase in concentration of total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) was found among CVD patients with MPO ˃ 44.3 ng/ml (P=0.01 and 0.019, respectively). Serum MPO showed significant positive correlation with TC and LDL-C (P=0.004 and 0.002, respectively). The UCP2-866 (rs659366) (GA and AA) genotype variants did not demonstrate statistically significant higher risk to develop cardiovascular events, yet were observed to have higher frequency among CVD patients and among patients with MPO ˃ 44.3ng/ml. In conclusion, our results revealed significant correlation between serum myeloperoxidase and CVD lipid risk factors in diabetic patients. This study suggests UCP2 -866G/A (rs659366) gene polymorphism and MPO as target assay studies on CVD etiology and oxidative stress parameters evaluation among type 2 diabetic patients. |
| first_indexed | 2024-04-10T07:01:22Z |
| format | Article |
| id | doaj.art-25a0acc9407e4150aae773c1523e4fcd |
| institution | Directory Open Access Journal |
| issn | 2682-4396 2682-440X |
| language | English |
| last_indexed | 2024-04-10T07:01:22Z |
| publishDate | 2023-01-01 |
| publisher | Beni-Suef University, Faculty of Medicine |
| record_format | Article |
| series | Egyptian Journal of Medical Research |
| spelling | doaj.art-25a0acc9407e4150aae773c1523e4fcd2023-02-27T17:41:55ZengBeni-Suef University, Faculty of MedicineEgyptian Journal of Medical Research2682-43962682-440X2023-01-014111413210.21608/ejmr.2022.181672.1303287657Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patientsHanan Mohamed Farhan0Khadiga Abo Gabl1Iman Atef Mandour2Heba Nabil Baz3Neveen Ahmed Khalil4Mahmoud Nassar5Mohamed aly Gabr6Clinical and Chemical pathology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, EgyptClinical Pathology Department, Faculty of Medicine, Beni-Suef University, EgyptClinical & Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, EgyptClinical & Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, EgyptClinical & Chemical Pathology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, EgyptInternal Medicine Department, Icahn School of Medicine at Mount Sinai, NYC Health+Hospital, Queens, New York, USAChest Unit, Ahmed Maher Teaching Hospital, Cairo, EgyptDiabetic cardiovascular diseases are leading causes of death. In diabetic patients, oxidative stress is key event in cardiovascular disease (CVD) development. Uncoupling protein 2 (UCP2) and myeloperoxidase (MPO) were identified as molecules regulating oxidative stress effects of oxygen free radicals. This work aimed to evaluate association of UCP2-866G/A (rs659366) gene polymorphism and serum MPO with CVD among type 2 diabetic patients. The present work was conducted on 80 type 2 diabetic Egyptian patients including 40 patients with CVD compared to 40 age- and sex-matched non-CVD patients; recruited from Outpatient Clinics in Beni-suef University Hospital and Kasr Eleiny Hospital. Lipid profile was assayed. Serum MPO was analyzed using enzyme-linked immunosorbent assay. UCP2-866G/A (rs659366) gene polymorphism was determined using polymerase chain reaction-restriction fragment length polymorphism. Statistically significant increase in concentration of total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) was found among CVD patients with MPO ˃ 44.3 ng/ml (P=0.01 and 0.019, respectively). Serum MPO showed significant positive correlation with TC and LDL-C (P=0.004 and 0.002, respectively). The UCP2-866 (rs659366) (GA and AA) genotype variants did not demonstrate statistically significant higher risk to develop cardiovascular events, yet were observed to have higher frequency among CVD patients and among patients with MPO ˃ 44.3ng/ml. In conclusion, our results revealed significant correlation between serum myeloperoxidase and CVD lipid risk factors in diabetic patients. This study suggests UCP2 -866G/A (rs659366) gene polymorphism and MPO as target assay studies on CVD etiology and oxidative stress parameters evaluation among type 2 diabetic patients.https://ejmr.journals.ekb.eg/article_287657_d41d8cd98f00b204e9800998ecf8427e.pdfcardiovascular diseaseoxidative stressmyeloperoxidaseuncoupling protein 2ucp2-866g/a (rs659366) gene polymorphism |
| spellingShingle | Hanan Mohamed Farhan Khadiga Abo Gabl Iman Atef Mandour Heba Nabil Baz Neveen Ahmed Khalil Mahmoud Nassar Mohamed aly Gabr Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients Egyptian Journal of Medical Research cardiovascular disease oxidative stress myeloperoxidase uncoupling protein 2 ucp2-866g/a (rs659366) gene polymorphism |
| title | Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients |
| title_full | Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients |
| title_fullStr | Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients |
| title_full_unstemmed | Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients |
| title_short | Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients |
| title_sort | association study of uncoupling protein 2 866g a rs659366 gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients |
| topic | cardiovascular disease oxidative stress myeloperoxidase uncoupling protein 2 ucp2-866g/a (rs659366) gene polymorphism |
| url | https://ejmr.journals.ekb.eg/article_287657_d41d8cd98f00b204e9800998ecf8427e.pdf |
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