The effects of wild-type and mutant SOD1 on smooth muscle contraction
In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2015-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400023N.pdf |
| Summary: | In this work we compared the mutated liver copper zinc-containing superoxide
dismutase (SOD1) protein G93A of the transgenic rat model of familial
amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined
their enzymatic activities and effects on isometric contractions of uteri of
healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1
and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle
relaxation. This result indicates that effects on smooth muscles are not
related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1
form an ion-conducting pore that diminishes the relaxatory effects of SOD1.
We propose that this type of pathogenic feedback affects neurons in FALS.
[Projekat Ministarstva nauke Republike Srbije, br. 173014 i br. 175083] |
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| ISSN: | 0354-4664 1821-4339 |