The effects of wild-type and mutant SOD1 on smooth muscle contraction
In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2015-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400023N.pdf |
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| author | Nikolić-Kokić Aleksandra Oreščanin-Dušić Zorana Spasojević Ivan Blagojević Duško Stević Zorica Anđus Pavle Spasić Mihajlo |
| author_facet | Nikolić-Kokić Aleksandra Oreščanin-Dušić Zorana Spasojević Ivan Blagojević Duško Stević Zorica Anđus Pavle Spasić Mihajlo |
| author_sort | Nikolić-Kokić Aleksandra |
| collection | DOAJ |
| description | In this work we compared the mutated liver copper zinc-containing superoxide
dismutase (SOD1) protein G93A of the transgenic rat model of familial
amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined
their enzymatic activities and effects on isometric contractions of uteri of
healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1
and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle
relaxation. This result indicates that effects on smooth muscles are not
related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1
form an ion-conducting pore that diminishes the relaxatory effects of SOD1.
We propose that this type of pathogenic feedback affects neurons in FALS.
[Projekat Ministarstva nauke Republike Srbije, br. 173014 i br. 175083] |
| first_indexed | 2024-04-12T14:08:31Z |
| format | Article |
| id | doaj.art-25ac940fd1314be281d8b4d90734f018 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-12T14:08:31Z |
| publishDate | 2015-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-25ac940fd1314be281d8b4d90734f0182022-12-22T03:29:59ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392015-01-0167118719210.2298/ABS141006023N0354-46641400023NThe effects of wild-type and mutant SOD1 on smooth muscle contractionNikolić-Kokić Aleksandra0Oreščanin-Dušić Zorana1Spasojević Ivan2Blagojević Duško3Stević Zorica4Anđus Pavle5Spasić Mihajlo6Institute for Biological Research, Department of Physiology, BelgradeInstitute for Biological Research, Department of Physiology, BelgradeInstitute for Multidisciplinary Research, Life Sciences Department, BelgradeInstitute for Biological Research, Department of Physiology, BelgradeSchool of Medicine, Institute of Neurology, BelgradeFaculty of Biology, Center for Laser Microscopy, BelgradeInstitute for Biological Research, Department of Physiology, BelgradeIn this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS. [Projekat Ministarstva nauke Republike Srbije, br. 173014 i br. 175083]http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400023N.pdfFALSmutationG93A SOD1WT SOD1muscle relaxation |
| spellingShingle | Nikolić-Kokić Aleksandra Oreščanin-Dušić Zorana Spasojević Ivan Blagojević Duško Stević Zorica Anđus Pavle Spasić Mihajlo The effects of wild-type and mutant SOD1 on smooth muscle contraction Archives of Biological Sciences FALS mutation G93A SOD1 WT SOD1 muscle relaxation |
| title | The effects of wild-type and mutant SOD1 on smooth muscle contraction |
| title_full | The effects of wild-type and mutant SOD1 on smooth muscle contraction |
| title_fullStr | The effects of wild-type and mutant SOD1 on smooth muscle contraction |
| title_full_unstemmed | The effects of wild-type and mutant SOD1 on smooth muscle contraction |
| title_short | The effects of wild-type and mutant SOD1 on smooth muscle contraction |
| title_sort | effects of wild type and mutant sod1 on smooth muscle contraction |
| topic | FALS mutation G93A SOD1 WT SOD1 muscle relaxation |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400023N.pdf |
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