Differential Expression of Subsets of Genes Related to HDL Metabolism and Atherogenesis in the Peripheral Blood in Coronary Artery Disease
Differential expression of genes (DEGs) in coronary artery disease (CAD) and the association between transcript level and high-density lipoprotein cholesterol (HDL-C) were studied with 76 male patients with CAD and 63 control patients. The transcript level of genes related to HDL metabolism (24 gene...
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MDPI AG
2023-08-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/8/431 |
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| author | Alexander D. Dergunov Elena V. Nosova Alexandra V. Rozhkova Margarita A. Vinogradina Veronika B. Baserova Mikhail A. Popov Svetlana A. Limborska Liudmila V. Dergunova |
| author_facet | Alexander D. Dergunov Elena V. Nosova Alexandra V. Rozhkova Margarita A. Vinogradina Veronika B. Baserova Mikhail A. Popov Svetlana A. Limborska Liudmila V. Dergunova |
| author_sort | Alexander D. Dergunov |
| collection | DOAJ |
| description | Differential expression of genes (DEGs) in coronary artery disease (CAD) and the association between transcript level and high-density lipoprotein cholesterol (HDL-C) were studied with 76 male patients with CAD and 63 control patients. The transcript level of genes related to HDL metabolism (24 genes) and atherosclerosis-prone (41 genes) in RNA isolated from peripheral blood mononuclear cells was measured by real-time RT-PCR. Twenty-eight DEGs were identified. The expression of cholesterol transporters, <i>ALB</i>, <i>APOA1</i>, and <i>LCAT</i> was down-regulated, while the expression of <i>AMN</i>, <i>APOE</i>, <i>LDLR</i>, <i>LPL</i>, <i>PLTP</i>, <i>PRKACA</i>, and <i>CETP</i> was up-regulated. The systemic inflammation in CAD is evidenced by the up-regulation of <i>IL1B</i>, <i>TLR8, CXCL5</i>, and <i>TNFRSF1A</i>. For the controls, <i>TLR8</i> and <i>SOAT1</i> were negative predictors of the HDL-C level. For CAD patients, <i>PRKACG</i>, <i>PRKCQ</i>, and <i>SREBF1</i> were positive predictors, while <i>PRKACB</i>, <i>LCAT</i>, and <i>S100A8</i> were negative predictors. For CAD patients, the efficiency of reverse cholesterol transport is 73–79%, and intracellular free cholesterol seems to accumulate at hyperalphalipoproteinemia. Both atheroprotective (via <i>S100A8</i>) and proatherogenic (via <i>SREBF1</i>, <i>LCAT</i>, <i>PRKACG</i>, <i>PRKACB</i>, and <i>PRKCQ</i>) associations of gene expression with HDL-C determine HDL functionality in CAD patients. The selected key genes and involved pathways may represent HDL-specific targets for the diagnosis and treatment of CAD and atherosclerosis. |
| first_indexed | 2024-03-11T00:00:57Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-11T00:00:57Z |
| publishDate | 2023-08-01 |
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| spelling | doaj.art-25aeb1fae43640e9b42a0e5f2d2a4d3f2023-11-19T00:42:09ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-08-014586823684110.3390/cimb45080431Differential Expression of Subsets of Genes Related to HDL Metabolism and Atherogenesis in the Peripheral Blood in Coronary Artery DiseaseAlexander D. Dergunov0Elena V. Nosova1Alexandra V. Rozhkova2Margarita A. Vinogradina3Veronika B. Baserova4Mikhail A. Popov5Svetlana A. Limborska6Liudmila V. Dergunova7National Medical Research Center for Therapy and Preventive Medicine, Petroverigsky Street 10, Moscow 101990, RussiaLaboratory of Human Molecular Genetics, National Research Center “Kurchatov Institute”, Kurchatov Sq. 2, Moscow 123182, RussiaLaboratory of Human Molecular Genetics, National Research Center “Kurchatov Institute”, Kurchatov Sq. 2, Moscow 123182, RussiaLaboratory of Human Molecular Genetics, National Research Center “Kurchatov Institute”, Kurchatov Sq. 2, Moscow 123182, RussiaNational Medical Research Center for Therapy and Preventive Medicine, Petroverigsky Street 10, Moscow 101990, RussiaMoscow Regional Research and Clinical Institute MONIKI, Moscow 129110, RussiaLaboratory of Human Molecular Genetics, National Research Center “Kurchatov Institute”, Kurchatov Sq. 2, Moscow 123182, RussiaLaboratory of Human Molecular Genetics, National Research Center “Kurchatov Institute”, Kurchatov Sq. 2, Moscow 123182, RussiaDifferential expression of genes (DEGs) in coronary artery disease (CAD) and the association between transcript level and high-density lipoprotein cholesterol (HDL-C) were studied with 76 male patients with CAD and 63 control patients. The transcript level of genes related to HDL metabolism (24 genes) and atherosclerosis-prone (41 genes) in RNA isolated from peripheral blood mononuclear cells was measured by real-time RT-PCR. Twenty-eight DEGs were identified. The expression of cholesterol transporters, <i>ALB</i>, <i>APOA1</i>, and <i>LCAT</i> was down-regulated, while the expression of <i>AMN</i>, <i>APOE</i>, <i>LDLR</i>, <i>LPL</i>, <i>PLTP</i>, <i>PRKACA</i>, and <i>CETP</i> was up-regulated. The systemic inflammation in CAD is evidenced by the up-regulation of <i>IL1B</i>, <i>TLR8, CXCL5</i>, and <i>TNFRSF1A</i>. For the controls, <i>TLR8</i> and <i>SOAT1</i> were negative predictors of the HDL-C level. For CAD patients, <i>PRKACG</i>, <i>PRKCQ</i>, and <i>SREBF1</i> were positive predictors, while <i>PRKACB</i>, <i>LCAT</i>, and <i>S100A8</i> were negative predictors. For CAD patients, the efficiency of reverse cholesterol transport is 73–79%, and intracellular free cholesterol seems to accumulate at hyperalphalipoproteinemia. Both atheroprotective (via <i>S100A8</i>) and proatherogenic (via <i>SREBF1</i>, <i>LCAT</i>, <i>PRKACG</i>, <i>PRKACB</i>, and <i>PRKCQ</i>) associations of gene expression with HDL-C determine HDL functionality in CAD patients. The selected key genes and involved pathways may represent HDL-specific targets for the diagnosis and treatment of CAD and atherosclerosis.https://www.mdpi.com/1467-3045/45/8/431atherosclerosiscoronary artery diseasedifferentially expressed genesHDL metabolisminflammationtranscript |
| spellingShingle | Alexander D. Dergunov Elena V. Nosova Alexandra V. Rozhkova Margarita A. Vinogradina Veronika B. Baserova Mikhail A. Popov Svetlana A. Limborska Liudmila V. Dergunova Differential Expression of Subsets of Genes Related to HDL Metabolism and Atherogenesis in the Peripheral Blood in Coronary Artery Disease Current Issues in Molecular Biology atherosclerosis coronary artery disease differentially expressed genes HDL metabolism inflammation transcript |
| title | Differential Expression of Subsets of Genes Related to HDL Metabolism and Atherogenesis in the Peripheral Blood in Coronary Artery Disease |
| title_full | Differential Expression of Subsets of Genes Related to HDL Metabolism and Atherogenesis in the Peripheral Blood in Coronary Artery Disease |
| title_fullStr | Differential Expression of Subsets of Genes Related to HDL Metabolism and Atherogenesis in the Peripheral Blood in Coronary Artery Disease |
| title_full_unstemmed | Differential Expression of Subsets of Genes Related to HDL Metabolism and Atherogenesis in the Peripheral Blood in Coronary Artery Disease |
| title_short | Differential Expression of Subsets of Genes Related to HDL Metabolism and Atherogenesis in the Peripheral Blood in Coronary Artery Disease |
| title_sort | differential expression of subsets of genes related to hdl metabolism and atherogenesis in the peripheral blood in coronary artery disease |
| topic | atherosclerosis coronary artery disease differentially expressed genes HDL metabolism inflammation transcript |
| url | https://www.mdpi.com/1467-3045/45/8/431 |
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