Rapid measurement of serum caffeine concentrations in acuteclinical settings
AbstractThe number of acute caffeine poisoning cases have increased in Japan. We can use serum caffeine concentrations to evaluate the severity of caffeine poisoning and determine whether or not we should perform hemodialysis. In this study, we sought to develop a rapid method for measuring serum ca...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-11-01
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| Series: | Toxicology Communications |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/24734306.2021.1928366 |
| _version_ | 1797300139736432640 |
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| author | Tomoki Hanazawa Yoshito Kamijo Tomohiro Yoshizawa Kiyotaka Usui |
| author_facet | Tomoki Hanazawa Yoshito Kamijo Tomohiro Yoshizawa Kiyotaka Usui |
| author_sort | Tomoki Hanazawa |
| collection | DOAJ |
| description | AbstractThe number of acute caffeine poisoning cases have increased in Japan. We can use serum caffeine concentrations to evaluate the severity of caffeine poisoning and determine whether or not we should perform hemodialysis. In this study, we sought to develop a rapid method for measuring serum caffeine concentrations. We used liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the new method. We chose caffeine-d9 as the internal standard, and we used the standard addition method to quantify caffeine concentrations. We collected six blood samples from three patients with acute caffeine poisoning to measure serum caffeine concentrations. In our method, retention time for caffeine was 0.4 min, and the time required for the total LC-MS/MS analysis was 1 min per sample. We obtained accurate serum caffeine concentrations 7 min after injection into the LC-MS/MS instrument. Further, time-consuming sample pretreatment was not required because each sample was diluted 10,000-fold. As a result, we could obtain serum caffeine concentrations for each patient in a total of 40 min. Our findings suggest that rapid, accurate measurement of serum caffeine concentrations by LC-MS/MS could contribute to real-time evaluation of poisoning severity and determination of appropriate therapeutic strategies in acute clinical settings. |
| first_indexed | 2024-03-07T23:02:56Z |
| format | Article |
| id | doaj.art-25b60631aaeb44479719638a842eaf15 |
| institution | Directory Open Access Journal |
| issn | 2473-4306 |
| language | English |
| last_indexed | 2024-03-07T23:02:56Z |
| publishDate | 2021-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Toxicology Communications |
| spelling | doaj.art-25b60631aaeb44479719638a842eaf152024-02-22T09:16:09ZengTaylor & Francis GroupToxicology Communications2473-43062021-11-01519710110.1080/24734306.2021.1928366Rapid measurement of serum caffeine concentrations in acuteclinical settingsTomoki Hanazawa0Yoshito Kamijo1Tomohiro Yoshizawa2Kiyotaka Usui3Emergency Center and Poison Center, Saitama Medical University Hospital, Moroyama, Saitama, JapanEmergency Center and Poison Center, Saitama Medical University Hospital, Moroyama, Saitama, JapanEmergency Center and Poison Center, Saitama Medical University Hospital, Moroyama, Saitama, JapanEmergency Center and Poison Center, Saitama Medical University Hospital, Moroyama, Saitama, JapanAbstractThe number of acute caffeine poisoning cases have increased in Japan. We can use serum caffeine concentrations to evaluate the severity of caffeine poisoning and determine whether or not we should perform hemodialysis. In this study, we sought to develop a rapid method for measuring serum caffeine concentrations. We used liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the new method. We chose caffeine-d9 as the internal standard, and we used the standard addition method to quantify caffeine concentrations. We collected six blood samples from three patients with acute caffeine poisoning to measure serum caffeine concentrations. In our method, retention time for caffeine was 0.4 min, and the time required for the total LC-MS/MS analysis was 1 min per sample. We obtained accurate serum caffeine concentrations 7 min after injection into the LC-MS/MS instrument. Further, time-consuming sample pretreatment was not required because each sample was diluted 10,000-fold. As a result, we could obtain serum caffeine concentrations for each patient in a total of 40 min. Our findings suggest that rapid, accurate measurement of serum caffeine concentrations by LC-MS/MS could contribute to real-time evaluation of poisoning severity and determination of appropriate therapeutic strategies in acute clinical settings.https://www.tandfonline.com/doi/10.1080/24734306.2021.1928366Caffeinequantitationliquid chromatography-tandem mass spectrometryacute clinical settings |
| spellingShingle | Tomoki Hanazawa Yoshito Kamijo Tomohiro Yoshizawa Kiyotaka Usui Rapid measurement of serum caffeine concentrations in acuteclinical settings Toxicology Communications Caffeine quantitation liquid chromatography-tandem mass spectrometry acute clinical settings |
| title | Rapid measurement of serum caffeine concentrations in acuteclinical settings |
| title_full | Rapid measurement of serum caffeine concentrations in acuteclinical settings |
| title_fullStr | Rapid measurement of serum caffeine concentrations in acuteclinical settings |
| title_full_unstemmed | Rapid measurement of serum caffeine concentrations in acuteclinical settings |
| title_short | Rapid measurement of serum caffeine concentrations in acuteclinical settings |
| title_sort | rapid measurement of serum caffeine concentrations in acuteclinical settings |
| topic | Caffeine quantitation liquid chromatography-tandem mass spectrometry acute clinical settings |
| url | https://www.tandfonline.com/doi/10.1080/24734306.2021.1928366 |
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