Prophylactic and therapeutic protection of human IgG purified from sera containing anti-exotoxin A titers against pneumonia caused by Pseudomonas aeruginosa
Antibodies are effective alternative tools to combat infections caused by Pseudomonas aeruginosa (PA), especially multi-drug-resistant PA. Thus, to solve the urgent need for an anti-PA antibody drug, we hypothesized that anti-PA intravenous immunoglobulins could be a practical attempt. Exotoxin A (E...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Human Vaccines & Immunotherapeutics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21645515.2019.1619404 |
| _version_ | 1827809698628239360 |
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| author | Jin Zhang Chuang Wan Bo Yu Chen Gao Liqun Zhao Xin Cheng Feng Yang Hao Gu Quanming Zou Jiang Gu Xingyong Wang |
| author_facet | Jin Zhang Chuang Wan Bo Yu Chen Gao Liqun Zhao Xin Cheng Feng Yang Hao Gu Quanming Zou Jiang Gu Xingyong Wang |
| author_sort | Jin Zhang |
| collection | DOAJ |
| description | Antibodies are effective alternative tools to combat infections caused by Pseudomonas aeruginosa (PA), especially multi-drug-resistant PA. Thus, to solve the urgent need for an anti-PA antibody drug, we hypothesized that anti-PA intravenous immunoglobulins could be a practical attempt. Exotoxin A (ETA) is one of the most important factors for PA infection and is also a critical target for the development of immune interventions. In this study, a total of 320 sera were collected from healthy volunteers. The concentration of ETA-specific antibodies was determined by a Luminex-based assay and then purified by affinity chromatography. The purified IgGs were able to neutralize the cytotoxicity of ETA in vitro. We showed they had a prophylactic and therapeutic protective effect in PA pneumonia and ETA toxemia models. In addition, administration of nonspecific IgGs also provided partial protection. Collectively, our results provide additional evidence for IVIG-based treatment of infections caused by multi-drug-resistant PA and suggest that patients at high risk of PA pneumonia could be prophylactically treated with anti-ETA IgGs or even with nonspecific IgGs. |
| first_indexed | 2024-03-11T22:44:27Z |
| format | Article |
| id | doaj.art-25b78ceca3b84b01b6e28757bc8a671c |
| institution | Directory Open Access Journal |
| issn | 2164-5515 2164-554X |
| language | English |
| last_indexed | 2024-03-11T22:44:27Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Human Vaccines & Immunotherapeutics |
| spelling | doaj.art-25b78ceca3b84b01b6e28757bc8a671c2023-09-22T08:45:31ZengTaylor & Francis GroupHuman Vaccines & Immunotherapeutics2164-55152164-554X2019-12-0115122993300210.1080/21645515.2019.16194041619404Prophylactic and therapeutic protection of human IgG purified from sera containing anti-exotoxin A titers against pneumonia caused by Pseudomonas aeruginosaJin Zhang0Chuang Wan1Bo Yu2Chen Gao3Liqun Zhao4Xin Cheng5Feng Yang6Hao Gu7Quanming Zou8Jiang Gu9Xingyong Wang10Children’s Hospital of Chongqing Medical UniversityCollege of Pharmacy, Third Military Medical UniversityThird Military Medical UniversityCollege of Pharmacy, Third Military Medical UniversityCollege of Pharmacy, Third Military Medical UniversityCollege of Pharmacy, Third Military Medical UniversityCollege of Pharmacy, Third Military Medical UniversityCollege of Pharmacy, Third Military Medical UniversityCollege of Pharmacy, Third Military Medical UniversityCollege of Pharmacy, Third Military Medical UniversityChildren’s Hospital of Chongqing Medical UniversityAntibodies are effective alternative tools to combat infections caused by Pseudomonas aeruginosa (PA), especially multi-drug-resistant PA. Thus, to solve the urgent need for an anti-PA antibody drug, we hypothesized that anti-PA intravenous immunoglobulins could be a practical attempt. Exotoxin A (ETA) is one of the most important factors for PA infection and is also a critical target for the development of immune interventions. In this study, a total of 320 sera were collected from healthy volunteers. The concentration of ETA-specific antibodies was determined by a Luminex-based assay and then purified by affinity chromatography. The purified IgGs were able to neutralize the cytotoxicity of ETA in vitro. We showed they had a prophylactic and therapeutic protective effect in PA pneumonia and ETA toxemia models. In addition, administration of nonspecific IgGs also provided partial protection. Collectively, our results provide additional evidence for IVIG-based treatment of infections caused by multi-drug-resistant PA and suggest that patients at high risk of PA pneumonia could be prophylactically treated with anti-ETA IgGs or even with nonspecific IgGs.http://dx.doi.org/10.1080/21645515.2019.1619404pseudomonas aeruginosaexotoxin ahuman intravenous immunoglobulintherapy |
| spellingShingle | Jin Zhang Chuang Wan Bo Yu Chen Gao Liqun Zhao Xin Cheng Feng Yang Hao Gu Quanming Zou Jiang Gu Xingyong Wang Prophylactic and therapeutic protection of human IgG purified from sera containing anti-exotoxin A titers against pneumonia caused by Pseudomonas aeruginosa Human Vaccines & Immunotherapeutics pseudomonas aeruginosa exotoxin a human intravenous immunoglobulin therapy |
| title | Prophylactic and therapeutic protection of human IgG purified from sera containing anti-exotoxin A titers against pneumonia caused by Pseudomonas aeruginosa |
| title_full | Prophylactic and therapeutic protection of human IgG purified from sera containing anti-exotoxin A titers against pneumonia caused by Pseudomonas aeruginosa |
| title_fullStr | Prophylactic and therapeutic protection of human IgG purified from sera containing anti-exotoxin A titers against pneumonia caused by Pseudomonas aeruginosa |
| title_full_unstemmed | Prophylactic and therapeutic protection of human IgG purified from sera containing anti-exotoxin A titers against pneumonia caused by Pseudomonas aeruginosa |
| title_short | Prophylactic and therapeutic protection of human IgG purified from sera containing anti-exotoxin A titers against pneumonia caused by Pseudomonas aeruginosa |
| title_sort | prophylactic and therapeutic protection of human igg purified from sera containing anti exotoxin a titers against pneumonia caused by pseudomonas aeruginosa |
| topic | pseudomonas aeruginosa exotoxin a human intravenous immunoglobulin therapy |
| url | http://dx.doi.org/10.1080/21645515.2019.1619404 |
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