Exploring Transcriptional Regulation of Hyperaccumulation in <i>Sedum plumbizincicola</i> through Integrated Transcriptome Analysis and CRISPR/Cas9 Technology
The cadmium hyperaccumulator <i>Sedum plumbizincicola</i> has remarkable abilities for cadmium (Cd) transport, accumulation and detoxification, but the transcriptional regulation mechanisms responsible for its Cd hyperaccumulation remain unknown. To address this knowledge gap, we conduct...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/14/11845 |
| Summary: | The cadmium hyperaccumulator <i>Sedum plumbizincicola</i> has remarkable abilities for cadmium (Cd) transport, accumulation and detoxification, but the transcriptional regulation mechanisms responsible for its Cd hyperaccumulation remain unknown. To address this knowledge gap, we conducted a comparative transcriptome study between <i>S. plumbizincicola</i> and the non-hyperaccumulating ecotype (NHE) of <i>Sedum alfredii</i> with or without Cd treatment. Our results revealed many differentially expressed genes involved in heavy metal transport and detoxification that were abundantly expressed in <i>S. plumbizincicola</i>. Additionally, we identified a large number of differentially expressed transcription factor genes, highlighting the complexity of transcriptional regulatory networks. We further screened four transcription factor genes that were highly expressed in the roots of <i>S. plumbizincicola</i> as candidate genes for creating CRISPR/Cas9 knockout mutations. Among these, the <i>SpARR11</i> and <i>SpMYB84</i> mutant lines exhibited decreased Cd accumulation in their aboveground parts, suggesting that these two transcription factors may play a role in the regulation of the Cd hyperaccumulation in <i>S. plumbizincicola</i>. Although further research will be required to determine the precise targeted genes of these transcription factors, combined transcriptome analysis and CRISPR/Cas9 technology provides unprecedented opportunities for identifying transcription factors related to Cd hyperaccumulation and contributes to the understanding of the transcriptional regulation mechanism of hyperaccumulation in <i>S. plumbizincicola</i>. |
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| ISSN: | 1661-6596 1422-0067 |