Exploring Transcriptional Regulation of Hyperaccumulation in <i>Sedum plumbizincicola</i> through Integrated Transcriptome Analysis and CRISPR/Cas9 Technology
The cadmium hyperaccumulator <i>Sedum plumbizincicola</i> has remarkable abilities for cadmium (Cd) transport, accumulation and detoxification, but the transcriptional regulation mechanisms responsible for its Cd hyperaccumulation remain unknown. To address this knowledge gap, we conduct...
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/14/11845 |
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| author | Yixin Zhang Yanlan Mo Liyuan Han Zhenyuan Sun Wenzhong Xu |
| author_facet | Yixin Zhang Yanlan Mo Liyuan Han Zhenyuan Sun Wenzhong Xu |
| author_sort | Yixin Zhang |
| collection | DOAJ |
| description | The cadmium hyperaccumulator <i>Sedum plumbizincicola</i> has remarkable abilities for cadmium (Cd) transport, accumulation and detoxification, but the transcriptional regulation mechanisms responsible for its Cd hyperaccumulation remain unknown. To address this knowledge gap, we conducted a comparative transcriptome study between <i>S. plumbizincicola</i> and the non-hyperaccumulating ecotype (NHE) of <i>Sedum alfredii</i> with or without Cd treatment. Our results revealed many differentially expressed genes involved in heavy metal transport and detoxification that were abundantly expressed in <i>S. plumbizincicola</i>. Additionally, we identified a large number of differentially expressed transcription factor genes, highlighting the complexity of transcriptional regulatory networks. We further screened four transcription factor genes that were highly expressed in the roots of <i>S. plumbizincicola</i> as candidate genes for creating CRISPR/Cas9 knockout mutations. Among these, the <i>SpARR11</i> and <i>SpMYB84</i> mutant lines exhibited decreased Cd accumulation in their aboveground parts, suggesting that these two transcription factors may play a role in the regulation of the Cd hyperaccumulation in <i>S. plumbizincicola</i>. Although further research will be required to determine the precise targeted genes of these transcription factors, combined transcriptome analysis and CRISPR/Cas9 technology provides unprecedented opportunities for identifying transcription factors related to Cd hyperaccumulation and contributes to the understanding of the transcriptional regulation mechanism of hyperaccumulation in <i>S. plumbizincicola</i>. |
| first_indexed | 2024-03-11T00:59:25Z |
| format | Article |
| id | doaj.art-25bb6f1a6b5249338ec325ba5229d97c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:59:25Z |
| publishDate | 2023-07-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-25bb6f1a6b5249338ec325ba5229d97c2023-11-18T19:45:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124141184510.3390/ijms241411845Exploring Transcriptional Regulation of Hyperaccumulation in <i>Sedum plumbizincicola</i> through Integrated Transcriptome Analysis and CRISPR/Cas9 TechnologyYixin Zhang0Yanlan Mo1Liyuan Han2Zhenyuan Sun3Wenzhong Xu4State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaKey Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaKey Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaThe cadmium hyperaccumulator <i>Sedum plumbizincicola</i> has remarkable abilities for cadmium (Cd) transport, accumulation and detoxification, but the transcriptional regulation mechanisms responsible for its Cd hyperaccumulation remain unknown. To address this knowledge gap, we conducted a comparative transcriptome study between <i>S. plumbizincicola</i> and the non-hyperaccumulating ecotype (NHE) of <i>Sedum alfredii</i> with or without Cd treatment. Our results revealed many differentially expressed genes involved in heavy metal transport and detoxification that were abundantly expressed in <i>S. plumbizincicola</i>. Additionally, we identified a large number of differentially expressed transcription factor genes, highlighting the complexity of transcriptional regulatory networks. We further screened four transcription factor genes that were highly expressed in the roots of <i>S. plumbizincicola</i> as candidate genes for creating CRISPR/Cas9 knockout mutations. Among these, the <i>SpARR11</i> and <i>SpMYB84</i> mutant lines exhibited decreased Cd accumulation in their aboveground parts, suggesting that these two transcription factors may play a role in the regulation of the Cd hyperaccumulation in <i>S. plumbizincicola</i>. Although further research will be required to determine the precise targeted genes of these transcription factors, combined transcriptome analysis and CRISPR/Cas9 technology provides unprecedented opportunities for identifying transcription factors related to Cd hyperaccumulation and contributes to the understanding of the transcriptional regulation mechanism of hyperaccumulation in <i>S. plumbizincicola</i>.https://www.mdpi.com/1422-0067/24/14/11845cadmiumCRISPR/Cas9hyperaccumulationtranscription factor<i>Sedum plumbizincicola</i>transcriptome sequencing |
| spellingShingle | Yixin Zhang Yanlan Mo Liyuan Han Zhenyuan Sun Wenzhong Xu Exploring Transcriptional Regulation of Hyperaccumulation in <i>Sedum plumbizincicola</i> through Integrated Transcriptome Analysis and CRISPR/Cas9 Technology International Journal of Molecular Sciences cadmium CRISPR/Cas9 hyperaccumulation transcription factor <i>Sedum plumbizincicola</i> transcriptome sequencing |
| title | Exploring Transcriptional Regulation of Hyperaccumulation in <i>Sedum plumbizincicola</i> through Integrated Transcriptome Analysis and CRISPR/Cas9 Technology |
| title_full | Exploring Transcriptional Regulation of Hyperaccumulation in <i>Sedum plumbizincicola</i> through Integrated Transcriptome Analysis and CRISPR/Cas9 Technology |
| title_fullStr | Exploring Transcriptional Regulation of Hyperaccumulation in <i>Sedum plumbizincicola</i> through Integrated Transcriptome Analysis and CRISPR/Cas9 Technology |
| title_full_unstemmed | Exploring Transcriptional Regulation of Hyperaccumulation in <i>Sedum plumbizincicola</i> through Integrated Transcriptome Analysis and CRISPR/Cas9 Technology |
| title_short | Exploring Transcriptional Regulation of Hyperaccumulation in <i>Sedum plumbizincicola</i> through Integrated Transcriptome Analysis and CRISPR/Cas9 Technology |
| title_sort | exploring transcriptional regulation of hyperaccumulation in i sedum plumbizincicola i through integrated transcriptome analysis and crispr cas9 technology |
| topic | cadmium CRISPR/Cas9 hyperaccumulation transcription factor <i>Sedum plumbizincicola</i> transcriptome sequencing |
| url | https://www.mdpi.com/1422-0067/24/14/11845 |
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