Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates
A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2003-02-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/9/2/02-0246_article |
| _version_ | 1818284828511961088 |
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| author | Wieland Meyer Alexandra Castañeda Stuart Jackson Matthew Huynh Elizabeth Castañeda |
| author_facet | Wieland Meyer Alexandra Castañeda Stuart Jackson Matthew Huynh Elizabeth Castañeda |
| author_sort | Wieland Meyer |
| collection | DOAJ |
| description | A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with HhaI and Sau96I in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gattii serotypes B and C isolates. |
| first_indexed | 2024-12-13T00:59:00Z |
| format | Article |
| id | doaj.art-25bd62bef9064911adc84982dd8e7f53 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-13T00:59:00Z |
| publishDate | 2003-02-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-25bd62bef9064911adc84982dd8e7f532022-12-22T00:04:44ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592003-02-019218919510.3201/eid0902.020246Molecular Typing of IberoAmerican Cryptococcus neoformans IsolatesWieland MeyerAlexandra CastañedaStuart JacksonMatthew HuynhElizabeth CastañedaA network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with HhaI and Sau96I in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gattii serotypes B and C isolates.https://wwwnc.cdc.gov/eid/article/9/2/02-0246_articleCryptococcus neoformansmolecular epidemiologyPCR-fingerprintingURA5 – RFLP analysisresearchAustralia |
| spellingShingle | Wieland Meyer Alexandra Castañeda Stuart Jackson Matthew Huynh Elizabeth Castañeda Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates Emerging Infectious Diseases Cryptococcus neoformans molecular epidemiology PCR-fingerprinting URA5 – RFLP analysis research Australia |
| title | Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates |
| title_full | Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates |
| title_fullStr | Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates |
| title_full_unstemmed | Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates |
| title_short | Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates |
| title_sort | molecular typing of iberoamerican cryptococcus neoformans isolates |
| topic | Cryptococcus neoformans molecular epidemiology PCR-fingerprinting URA5 – RFLP analysis research Australia |
| url | https://wwwnc.cdc.gov/eid/article/9/2/02-0246_article |
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