Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs
Group I self-splicing intron constitutes an important class of functional RNA molecules that can promote chemical transformation. Although the fundamental mechanism of the auto-excision from its precursor RNA has been established, convenient assay systems for its splicing activity are still useful f...
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MDPI AG
2016-11-01
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| Series: | Biology |
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| Online Access: | http://www.mdpi.com/2079-7737/5/4/43 |
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| author | Airi Furukawa Takahiro Tanaka Hiroyuki Furuta Shigeyoshi Matsumura Yoshiya Ikawa |
| author_facet | Airi Furukawa Takahiro Tanaka Hiroyuki Furuta Shigeyoshi Matsumura Yoshiya Ikawa |
| author_sort | Airi Furukawa |
| collection | DOAJ |
| description | Group I self-splicing intron constitutes an important class of functional RNA molecules that can promote chemical transformation. Although the fundamental mechanism of the auto-excision from its precursor RNA has been established, convenient assay systems for its splicing activity are still useful for a further understanding of its detailed mechanism and of its application. Because some host RNA sequences, to which group I introns inserted form stable three-dimensional (3D) structures, the effects of the 3D structures of exonic elements on the splicing efficiency of group I introns are important but not a fully investigated issue. We developed an assay system for group I intron self-splicing by employing a fluorescent aptamer RNA (spinach RNA) as a model exonic sequence inserted by the Tetrahymena group I intron. We investigated self-splicing of the intron from spinach RNA, serving as a model exonic sequence with a 3D structure. |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-12T19:22:31Z |
| publishDate | 2016-11-01 |
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| record_format | Article |
| series | Biology |
| spelling | doaj.art-25c2495b48f3424e93d0cc81a7c2629c2023-08-02T04:59:48ZengMDPI AGBiology2079-77372016-11-01544310.3390/biology5040043biology5040043Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAsAiri Furukawa0Takahiro Tanaka1Hiroyuki Furuta2Shigeyoshi Matsumura3Yoshiya Ikawa4Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 930-8555, JapanDepartment of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Moto-oka 744, Nishi-ku, Fukuoka 819-0395, JapanDepartment of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Moto-oka 744, Nishi-ku, Fukuoka 819-0395, JapanDepartment of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 930-8555, JapanDepartment of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 930-8555, JapanGroup I self-splicing intron constitutes an important class of functional RNA molecules that can promote chemical transformation. Although the fundamental mechanism of the auto-excision from its precursor RNA has been established, convenient assay systems for its splicing activity are still useful for a further understanding of its detailed mechanism and of its application. Because some host RNA sequences, to which group I introns inserted form stable three-dimensional (3D) structures, the effects of the 3D structures of exonic elements on the splicing efficiency of group I introns are important but not a fully investigated issue. We developed an assay system for group I intron self-splicing by employing a fluorescent aptamer RNA (spinach RNA) as a model exonic sequence inserted by the Tetrahymena group I intron. We investigated self-splicing of the intron from spinach RNA, serving as a model exonic sequence with a 3D structure.http://www.mdpi.com/2079-7737/5/4/43intronribozymesself-splicingspinach RNATetrahymena |
| spellingShingle | Airi Furukawa Takahiro Tanaka Hiroyuki Furuta Shigeyoshi Matsumura Yoshiya Ikawa Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs Biology intron ribozymes self-splicing spinach RNA Tetrahymena |
| title | Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs |
| title_full | Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs |
| title_fullStr | Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs |
| title_full_unstemmed | Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs |
| title_short | Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs |
| title_sort | use of a fluorescent aptamer rna as an exonic sequence to analyze self splicing ability of a group i intron from structured rnas |
| topic | intron ribozymes self-splicing spinach RNA Tetrahymena |
| url | http://www.mdpi.com/2079-7737/5/4/43 |
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