Abscisic Acid Inhibits Cortical Microtubules Reorganization and Enhances Ultraviolet-B Tolerance in <em>Arabidopsis thaliana</em>
Ultraviolet-B (UV-B) radiation is one of the important environmental factors limiting plant growth. Both abscisic acid (ABA) and microtubules have been previously reported to be involved in plant response to UV-B. However, whether there is a potential link between ABA and microtubules and the conseq...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/4/892 |
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| author | Lichun Shi Kun Lin Tongbing Su Fumei Shi |
| author_facet | Lichun Shi Kun Lin Tongbing Su Fumei Shi |
| author_sort | Lichun Shi |
| collection | DOAJ |
| description | Ultraviolet-B (UV-B) radiation is one of the important environmental factors limiting plant growth. Both abscisic acid (ABA) and microtubules have been previously reported to be involved in plant response to UV-B. However, whether there is a potential link between ABA and microtubules and the consequent signal transduction mechanism underlying plant response to UV-B radiation remains largely unclear. Here, by using <i>sad2-2</i> mutant plants (sensitive to ABA and drought) and exogenous application of ABA, we saw that ABA strengthens the adaptive response to UV-B stress in <i>Arabidopsis thaliana</i> (<i>A. thaliana</i>). The abnormal swelling root tips of ABA-deficient <i>aba3</i> mutants demonstrated that ABA deficiency aggravated the growth retardation imposed by UV-B radiation. In addition, the cortical microtubule arrays of the transition zones of the roots were examined in the <i>aba3</i> and <i>sad2-2</i> mutants with or without UV-B radiation. The observation revealed that UV-B remodels cortical microtubules, and high endogenous ABA can stabilize the microtubules and reduce their UV-B-induced reorganization. To further confirm the role of ABA on microtubule arrays, root growth and cortical microtubules were evaluated after exogenous ABA, taxol, and oryzalin feeding. The results suggested that ABA can promote root elongation by stabilizing the transverse cortical microtubules under UV-B stress conditions. We thus uncovered an important role of ABA, which bridges UV-B and plants’ adaptive response by remodeling the rearrangement of the cortical microtubules. |
| first_indexed | 2024-03-11T04:58:46Z |
| format | Article |
| id | doaj.art-25c4f92f84fa4560bd7f3d93c62ecc2a |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T04:58:46Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-25c4f92f84fa4560bd7f3d93c62ecc2a2023-11-17T19:24:02ZengMDPI AGGenes2073-44252023-04-0114489210.3390/genes14040892Abscisic Acid Inhibits Cortical Microtubules Reorganization and Enhances Ultraviolet-B Tolerance in <em>Arabidopsis thaliana</em>Lichun Shi0Kun Lin1Tongbing Su2Fumei Shi3School of Life Science, Liaocheng University, Liaocheng 252059, ChinaSchool of Life Science, Liaocheng University, Liaocheng 252059, ChinaNational Engineering Research Center for Vegetables, Beijing 100097, ChinaSchool of Life Science, Liaocheng University, Liaocheng 252059, ChinaUltraviolet-B (UV-B) radiation is one of the important environmental factors limiting plant growth. Both abscisic acid (ABA) and microtubules have been previously reported to be involved in plant response to UV-B. However, whether there is a potential link between ABA and microtubules and the consequent signal transduction mechanism underlying plant response to UV-B radiation remains largely unclear. Here, by using <i>sad2-2</i> mutant plants (sensitive to ABA and drought) and exogenous application of ABA, we saw that ABA strengthens the adaptive response to UV-B stress in <i>Arabidopsis thaliana</i> (<i>A. thaliana</i>). The abnormal swelling root tips of ABA-deficient <i>aba3</i> mutants demonstrated that ABA deficiency aggravated the growth retardation imposed by UV-B radiation. In addition, the cortical microtubule arrays of the transition zones of the roots were examined in the <i>aba3</i> and <i>sad2-2</i> mutants with or without UV-B radiation. The observation revealed that UV-B remodels cortical microtubules, and high endogenous ABA can stabilize the microtubules and reduce their UV-B-induced reorganization. To further confirm the role of ABA on microtubule arrays, root growth and cortical microtubules were evaluated after exogenous ABA, taxol, and oryzalin feeding. The results suggested that ABA can promote root elongation by stabilizing the transverse cortical microtubules under UV-B stress conditions. We thus uncovered an important role of ABA, which bridges UV-B and plants’ adaptive response by remodeling the rearrangement of the cortical microtubules.https://www.mdpi.com/2073-4425/14/4/892ultraviolet-Babscisic acidcortical microtubulesroot growth<i>Arabidopsis thaliana</i> |
| spellingShingle | Lichun Shi Kun Lin Tongbing Su Fumei Shi Abscisic Acid Inhibits Cortical Microtubules Reorganization and Enhances Ultraviolet-B Tolerance in <em>Arabidopsis thaliana</em> Genes ultraviolet-B abscisic acid cortical microtubules root growth <i>Arabidopsis thaliana</i> |
| title | Abscisic Acid Inhibits Cortical Microtubules Reorganization and Enhances Ultraviolet-B Tolerance in <em>Arabidopsis thaliana</em> |
| title_full | Abscisic Acid Inhibits Cortical Microtubules Reorganization and Enhances Ultraviolet-B Tolerance in <em>Arabidopsis thaliana</em> |
| title_fullStr | Abscisic Acid Inhibits Cortical Microtubules Reorganization and Enhances Ultraviolet-B Tolerance in <em>Arabidopsis thaliana</em> |
| title_full_unstemmed | Abscisic Acid Inhibits Cortical Microtubules Reorganization and Enhances Ultraviolet-B Tolerance in <em>Arabidopsis thaliana</em> |
| title_short | Abscisic Acid Inhibits Cortical Microtubules Reorganization and Enhances Ultraviolet-B Tolerance in <em>Arabidopsis thaliana</em> |
| title_sort | abscisic acid inhibits cortical microtubules reorganization and enhances ultraviolet b tolerance in em arabidopsis thaliana em |
| topic | ultraviolet-B abscisic acid cortical microtubules root growth <i>Arabidopsis thaliana</i> |
| url | https://www.mdpi.com/2073-4425/14/4/892 |
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