Molecular species composition of polar lipids from two microalgae Nitzschia palea and Scenedesmus costatus using HPLC-ESI-MS/MS
This study examines the polar lipid profiles of two freshwater algae, Scenedesmus costatus and Nitzschia palea. HILIC-ESI-MS/MS analysis was used to determine and quantify the major phospholipids and glycolipids, as well as their relative molecular species, extracted from the two microalgal cultures...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2023-09-01
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| Series: | PeerJ Analytical Chemistry |
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| Online Access: | https://peerj.com/articles/achem-27.pdf |
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| author | Nicolas Mazzella Mariem Fadhlaoui Aurélie Moreira Soizic Morin |
| author_facet | Nicolas Mazzella Mariem Fadhlaoui Aurélie Moreira Soizic Morin |
| author_sort | Nicolas Mazzella |
| collection | DOAJ |
| description | This study examines the polar lipid profiles of two freshwater algae, Scenedesmus costatus and Nitzschia palea. HILIC-ESI-MS/MS analysis was used to determine and quantify the major phospholipids and glycolipids, as well as their relative molecular species, extracted from the two microalgal cultures. Glycolipids were eluted first, followed by phospholipids partially co-eluting with a sulfoglycolipid. The fragmentation pattern in the negative ionization mode for galactolipids was studied, revealing the stereospecific distribution of fatty acids on the glycerol backbone. Green algae frequently include 18:3 fatty acid in both phospholipids and galactolipids, while monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) were more saturated and contained shorter acyls. The diatom phospholipids contained mainly molecular species with saturated or monounsaturated fatty acids, while MGDG and DGDG exhibited a higher proportion of polyunsaturated fatty acids, such as the unique and abundant MGDG (20:5/20:2). |
| first_indexed | 2024-03-12T01:14:26Z |
| format | Article |
| id | doaj.art-25c5dd7fff4a4831b66da8e34d0e5f81 |
| institution | Directory Open Access Journal |
| issn | 2691-6630 |
| language | English |
| last_indexed | 2024-03-12T01:14:26Z |
| publishDate | 2023-09-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ Analytical Chemistry |
| spelling | doaj.art-25c5dd7fff4a4831b66da8e34d0e5f812023-09-13T15:05:06ZengPeerJ Inc.PeerJ Analytical Chemistry2691-66302023-09-015e2710.7717/peerj-achem.27Molecular species composition of polar lipids from two microalgae Nitzschia palea and Scenedesmus costatus using HPLC-ESI-MS/MSNicolas Mazzella0Mariem Fadhlaoui1Aurélie Moreira2Soizic Morin3INRAE, UR EABX, Cestas, FranceINRS-ETE, Québec, CanadaINRAE, UR EABX, Cestas, FranceINRAE, UR EABX, Cestas, FranceThis study examines the polar lipid profiles of two freshwater algae, Scenedesmus costatus and Nitzschia palea. HILIC-ESI-MS/MS analysis was used to determine and quantify the major phospholipids and glycolipids, as well as their relative molecular species, extracted from the two microalgal cultures. Glycolipids were eluted first, followed by phospholipids partially co-eluting with a sulfoglycolipid. The fragmentation pattern in the negative ionization mode for galactolipids was studied, revealing the stereospecific distribution of fatty acids on the glycerol backbone. Green algae frequently include 18:3 fatty acid in both phospholipids and galactolipids, while monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) were more saturated and contained shorter acyls. The diatom phospholipids contained mainly molecular species with saturated or monounsaturated fatty acids, while MGDG and DGDG exhibited a higher proportion of polyunsaturated fatty acids, such as the unique and abundant MGDG (20:5/20:2).https://peerj.com/articles/achem-27.pdfGlycolipidsPhospholipidsFatty acidsMass spectrometryDiatomsChlorophyta |
| spellingShingle | Nicolas Mazzella Mariem Fadhlaoui Aurélie Moreira Soizic Morin Molecular species composition of polar lipids from two microalgae Nitzschia palea and Scenedesmus costatus using HPLC-ESI-MS/MS PeerJ Analytical Chemistry Glycolipids Phospholipids Fatty acids Mass spectrometry Diatoms Chlorophyta |
| title | Molecular species composition of polar lipids from two microalgae Nitzschia palea and Scenedesmus costatus using HPLC-ESI-MS/MS |
| title_full | Molecular species composition of polar lipids from two microalgae Nitzschia palea and Scenedesmus costatus using HPLC-ESI-MS/MS |
| title_fullStr | Molecular species composition of polar lipids from two microalgae Nitzschia palea and Scenedesmus costatus using HPLC-ESI-MS/MS |
| title_full_unstemmed | Molecular species composition of polar lipids from two microalgae Nitzschia palea and Scenedesmus costatus using HPLC-ESI-MS/MS |
| title_short | Molecular species composition of polar lipids from two microalgae Nitzschia palea and Scenedesmus costatus using HPLC-ESI-MS/MS |
| title_sort | molecular species composition of polar lipids from two microalgae nitzschia palea and scenedesmus costatus using hplc esi ms ms |
| topic | Glycolipids Phospholipids Fatty acids Mass spectrometry Diatoms Chlorophyta |
| url | https://peerj.com/articles/achem-27.pdf |
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