Molecular characterization of field infectious bursal disease virus isolates from Nigeria
Aim: To characterize field isolates of infectious bursal disease virus (IBDV) from outbreaks in nine states in Nigeria through reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis of portions of the VP2 and VP1 genes and to determine the presence or absence of reassortant v...
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| Format: | Article |
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Veterinary World
2016-12-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.9/December-2016/14.pdf |
| _version_ | 1818741421291601920 |
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| author | Ijeoma O. Nwagbo Ismaila Shittu Chika I. Nwosuh George O. Ezeifeka Frederick J. C. Odibo Linda O. Michel Daral J. Jackwood |
| author_facet | Ijeoma O. Nwagbo Ismaila Shittu Chika I. Nwosuh George O. Ezeifeka Frederick J. C. Odibo Linda O. Michel Daral J. Jackwood |
| author_sort | Ijeoma O. Nwagbo |
| collection | DOAJ |
| description | Aim: To characterize field isolates of infectious bursal disease virus (IBDV) from outbreaks in nine states in Nigeria through reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis of portions of the VP2 and VP1 genes and to determine the presence or absence of reassortant viruses.
Materials and Methods: A total of 377 bursa samples were collected from 201 suspected IBD outbreaks during 2009 to 2014 from nine states in Nigeria. Samples were subjected to RT-PCR using VP2 and VP1 gene specific primers, and the resulting PCR products were sequenced.
Results: A total of 143 samples were positive for IBDV by RT-PCR. These assays amplified a 743 bp fragment from nt 701 to 1444 in the IBDV VP2 hypervariable region (hvVP2) of segment A and a 722 bp fragment from nt 168 to 889 in the VP1 gene of segment B. RT-PCR products were sequenced, aligned and compared with reference IBDV sequences obtained from GenBank. All but one hvVP2 sequence showed similarity to very virulent IBDV (vvIBDV) reference strains, yet only 3 of the VP1 67 VP1 sequences showed similarity to the VP1 gene of vvIBDV. Phylogenetic analysis revealed a new lineage of Nigerian reassortant IBDV strains.
Conclusion: Phylogenetic analysis of partial sequences of genome segment A and B of IBDV in Nigeria confirmed the existence of vvIBDV in Nigeria. In addition, we noted the existence of reassortant IBDV strains with novel triplet amino acid motifs at positions 145, 146 and 147 in the reassorted Nigerian IBDV. |
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| spelling | doaj.art-25c5e35ab4a74baa9087e17ca305eb3c2022-12-21T21:24:54ZengVeterinary WorldVeterinary World0972-89882231-09162016-12-019121420142810.14202/vetworld.2016.1420-1428Molecular characterization of field infectious bursal disease virus isolates from NigeriaIjeoma O. Nwagbo0Ismaila Shittu1Chika I. Nwosuh2George O. Ezeifeka3Frederick J. C. Odibo4Linda O. Michel5Daral J. Jackwood6Department of Virology, Viral Research Division, National Veterinary Research Institute, Vom, Plateau State, Nigeria; Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University Awka, Anambra State, Nigeria; Department of Veterinary Preventive Medicine, Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, USA; aijay02@yahoo.comDepartment of Virology, Viral Research Division, National Veterinary Research Institute, Vom, Plateau State, Nigeria; ismaila.shittu@gmail.comDepartment of Virology, Viral Research Division, National Veterinary Research Institute, Vom, Plateau State, Nigeria; chikanwosuh@nvri.gov.ngDepartment of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, Micheal Okpara University of Agriculture Umudike, Abia State, Nigeria; ezeifekag@yahoo.comDepartment of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University Awka, Anambra State, Nigeria; profodibo@yahoo.comDepartment of Veterinary Preventive Medicine, Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, USA; michel.37@osu.eduDepartment of Veterinary Preventive Medicine, Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, USA; Jackwood.2@osu.eduAim: To characterize field isolates of infectious bursal disease virus (IBDV) from outbreaks in nine states in Nigeria through reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis of portions of the VP2 and VP1 genes and to determine the presence or absence of reassortant viruses. Materials and Methods: A total of 377 bursa samples were collected from 201 suspected IBD outbreaks during 2009 to 2014 from nine states in Nigeria. Samples were subjected to RT-PCR using VP2 and VP1 gene specific primers, and the resulting PCR products were sequenced. Results: A total of 143 samples were positive for IBDV by RT-PCR. These assays amplified a 743 bp fragment from nt 701 to 1444 in the IBDV VP2 hypervariable region (hvVP2) of segment A and a 722 bp fragment from nt 168 to 889 in the VP1 gene of segment B. RT-PCR products were sequenced, aligned and compared with reference IBDV sequences obtained from GenBank. All but one hvVP2 sequence showed similarity to very virulent IBDV (vvIBDV) reference strains, yet only 3 of the VP1 67 VP1 sequences showed similarity to the VP1 gene of vvIBDV. Phylogenetic analysis revealed a new lineage of Nigerian reassortant IBDV strains. Conclusion: Phylogenetic analysis of partial sequences of genome segment A and B of IBDV in Nigeria confirmed the existence of vvIBDV in Nigeria. In addition, we noted the existence of reassortant IBDV strains with novel triplet amino acid motifs at positions 145, 146 and 147 in the reassorted Nigerian IBDV.http://www.veterinaryworld.org/Vol.9/December-2016/14.pdfinfectious bursal disease virusNigerianovelreassortantvery virulent |
| spellingShingle | Ijeoma O. Nwagbo Ismaila Shittu Chika I. Nwosuh George O. Ezeifeka Frederick J. C. Odibo Linda O. Michel Daral J. Jackwood Molecular characterization of field infectious bursal disease virus isolates from Nigeria Veterinary World infectious bursal disease virus Nigeria novel reassortant very virulent |
| title | Molecular characterization of field infectious bursal disease virus isolates from Nigeria |
| title_full | Molecular characterization of field infectious bursal disease virus isolates from Nigeria |
| title_fullStr | Molecular characterization of field infectious bursal disease virus isolates from Nigeria |
| title_full_unstemmed | Molecular characterization of field infectious bursal disease virus isolates from Nigeria |
| title_short | Molecular characterization of field infectious bursal disease virus isolates from Nigeria |
| title_sort | molecular characterization of field infectious bursal disease virus isolates from nigeria |
| topic | infectious bursal disease virus Nigeria novel reassortant very virulent |
| url | http://www.veterinaryworld.org/Vol.9/December-2016/14.pdf |
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