The Characterization of Three Novel Insect-Specific Viruses Discovered in the Bean Bug, <i>Riptortus pedestris</i>
Insect-specific virus (ISV) is one of the most promising agents for the biological control of insects, which is abundantly distributed in hematophagous insects. However, few ISVs have been reported in <i>Riptortus pedestris</i> (Fabricius), one of the major pests threatening soybeans and...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/11/2500 |
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| author | Chunyun Guo Zhuangxin Ye Biao Hu Shiqi Shan Jianping Chen Zongtao Sun Junmin Li Zhongyan Wei |
| author_facet | Chunyun Guo Zhuangxin Ye Biao Hu Shiqi Shan Jianping Chen Zongtao Sun Junmin Li Zhongyan Wei |
| author_sort | Chunyun Guo |
| collection | DOAJ |
| description | Insect-specific virus (ISV) is one of the most promising agents for the biological control of insects, which is abundantly distributed in hematophagous insects. However, few ISVs have been reported in <i>Riptortus pedestris</i> (Fabricius), one of the major pests threatening soybeans and causing great losses in yield and quality. In this work, field <i>Riptortus pedestris</i> was collected from six soybean-producing regions in China, and their virome was analyzed with the metatranscriptomic approach. Altogether, seven new insect RNA viruses were identified, three of which had complete RNA-dependent RNA polymerase (RdRp) and nearly full-length genome sequences, which were named Riptortus pedestris alphadrosrha-like virus 1 (RpALv1), Riptortus pedestris alphadrosrha-like virus 2 (RpALv2) and Riptortus pedestris almendra-like virus (RiALv). The three identified novel ISVs belonged to the family Rhabdoviridae, and phylogenetic tree analysis indicated that they were clustered into new distinct clades. Interestingly, the analysis of virus-derived small-interfering RNAs (vsiRNAs) indicated that only RiALv-derived siRNAs exhibited 22 nt length preference, whereas no clear 21 or 22 nt peaks were observed for RpALv1 and RpALv2, suggesting the complexity of siRNA-based antiviral immunity in <i>R. pedestris</i>. In conclusion, this study contributes to a better understanding of the microenvironment in <i>R. pedestris</i> and provides viral information for the development of potential soybean insect-specific biocontrol agents. |
| first_indexed | 2024-03-09T17:55:56Z |
| format | Article |
| id | doaj.art-25c86964bb564080b74ac11cc914f188 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T17:55:56Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-25c86964bb564080b74ac11cc914f1882023-11-24T10:18:12ZengMDPI AGViruses1999-49152022-11-011411250010.3390/v14112500The Characterization of Three Novel Insect-Specific Viruses Discovered in the Bean Bug, <i>Riptortus pedestris</i>Chunyun Guo0Zhuangxin Ye1Biao Hu2Shiqi Shan3Jianping Chen4Zongtao Sun5Junmin Li6Zhongyan Wei7State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaInsect-specific virus (ISV) is one of the most promising agents for the biological control of insects, which is abundantly distributed in hematophagous insects. However, few ISVs have been reported in <i>Riptortus pedestris</i> (Fabricius), one of the major pests threatening soybeans and causing great losses in yield and quality. In this work, field <i>Riptortus pedestris</i> was collected from six soybean-producing regions in China, and their virome was analyzed with the metatranscriptomic approach. Altogether, seven new insect RNA viruses were identified, three of which had complete RNA-dependent RNA polymerase (RdRp) and nearly full-length genome sequences, which were named Riptortus pedestris alphadrosrha-like virus 1 (RpALv1), Riptortus pedestris alphadrosrha-like virus 2 (RpALv2) and Riptortus pedestris almendra-like virus (RiALv). The three identified novel ISVs belonged to the family Rhabdoviridae, and phylogenetic tree analysis indicated that they were clustered into new distinct clades. Interestingly, the analysis of virus-derived small-interfering RNAs (vsiRNAs) indicated that only RiALv-derived siRNAs exhibited 22 nt length preference, whereas no clear 21 or 22 nt peaks were observed for RpALv1 and RpALv2, suggesting the complexity of siRNA-based antiviral immunity in <i>R. pedestris</i>. In conclusion, this study contributes to a better understanding of the microenvironment in <i>R. pedestris</i> and provides viral information for the development of potential soybean insect-specific biocontrol agents.https://www.mdpi.com/1999-4915/14/11/2500<i>Riptortus pedestris</i>insect-specific virusmetatranscriptomic sequencingsmall interference RNA |
| spellingShingle | Chunyun Guo Zhuangxin Ye Biao Hu Shiqi Shan Jianping Chen Zongtao Sun Junmin Li Zhongyan Wei The Characterization of Three Novel Insect-Specific Viruses Discovered in the Bean Bug, <i>Riptortus pedestris</i> Viruses <i>Riptortus pedestris</i> insect-specific virus metatranscriptomic sequencing small interference RNA |
| title | The Characterization of Three Novel Insect-Specific Viruses Discovered in the Bean Bug, <i>Riptortus pedestris</i> |
| title_full | The Characterization of Three Novel Insect-Specific Viruses Discovered in the Bean Bug, <i>Riptortus pedestris</i> |
| title_fullStr | The Characterization of Three Novel Insect-Specific Viruses Discovered in the Bean Bug, <i>Riptortus pedestris</i> |
| title_full_unstemmed | The Characterization of Three Novel Insect-Specific Viruses Discovered in the Bean Bug, <i>Riptortus pedestris</i> |
| title_short | The Characterization of Three Novel Insect-Specific Viruses Discovered in the Bean Bug, <i>Riptortus pedestris</i> |
| title_sort | characterization of three novel insect specific viruses discovered in the bean bug i riptortus pedestris i |
| topic | <i>Riptortus pedestris</i> insect-specific virus metatranscriptomic sequencing small interference RNA |
| url | https://www.mdpi.com/1999-4915/14/11/2500 |
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