Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells
Objective: To investigate the in vitro anticancer effect of Aloe vera (A. vera) and Calligonum comosum (C. comosum) extracts against hepatocellular carcinoma (HepG2) cells. Methods: HepG2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-05-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115303725 |
| _version_ | 1819294223876227072 |
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| author | Maram Shalabi Kh. Khilo Mahmoud M. Zakaria Mahmoud G. Elsebaei Walied Abdo Walaa Awadin |
| author_facet | Maram Shalabi Kh. Khilo Mahmoud M. Zakaria Mahmoud G. Elsebaei Walied Abdo Walaa Awadin |
| author_sort | Maram Shalabi |
| collection | DOAJ |
| description | Objective: To investigate the in vitro anticancer effect of Aloe vera (A. vera) and Calligonum comosum (C. comosum) extracts against hepatocellular carcinoma (HepG2) cells.
Methods: HepG2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic (Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG2 cells were observed using light microscopy.
Results: The IC50 values of A. vera and C. comosum extracts were (10.45 ± 0.31) and (9.60 ± 0.01) µg/mL respectively. The extracts separately increased cytotoxicity against HepG2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions.
Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis. |
| first_indexed | 2024-12-24T04:22:55Z |
| format | Article |
| id | doaj.art-25ce092ae5c34a82be564bb405ac6908 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-24T04:22:55Z |
| publishDate | 2015-05-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-25ce092ae5c34a82be564bb405ac69082022-12-21T17:15:45ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912015-05-015537538110.1016/S2221-1691(15)30372-5Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cellsMaram Shalabi0Kh. Khilo1Mahmoud M. Zakaria2Mahmoud G. Elsebaei3Walied Abdo4Walaa Awadin5Department of Biochemistry, Faculty of Veterinary Medicine, Mansoura University, Ad Daqahliyah, EgyptDepartment of Biochemistry, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, EgyptUrology and Nephrology Center, Mansoura University, Ad Daqahliyah, EgyptDepartment of Biochemistry, Faculty of Veterinary Medicine, Mansoura University, Ad Daqahliyah, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, Mansoura University, Ad Daqahliyah, EgyptObjective: To investigate the in vitro anticancer effect of Aloe vera (A. vera) and Calligonum comosum (C. comosum) extracts against hepatocellular carcinoma (HepG2) cells. Methods: HepG2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic (Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG2 cells were observed using light microscopy. Results: The IC50 values of A. vera and C. comosum extracts were (10.45 ± 0.31) and (9.60 ± 0.01) µg/mL respectively. The extracts separately increased cytotoxicity against HepG2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions. Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis.http://www.sciencedirect.com/science/article/pii/S2221169115303725HepG2Hepatocellular carcinomaAloe veraCalligonum comosumApoptosis |
| spellingShingle | Maram Shalabi Kh. Khilo Mahmoud M. Zakaria Mahmoud G. Elsebaei Walied Abdo Walaa Awadin Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells Asian Pacific Journal of Tropical Biomedicine HepG2 Hepatocellular carcinoma Aloe vera Calligonum comosum Apoptosis |
| title | Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells |
| title_full | Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells |
| title_fullStr | Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells |
| title_full_unstemmed | Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells |
| title_short | Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells |
| title_sort | anticancer activity of aloe vera and calligonum comosum extracts separetely on hepatocellular carcinoma cells |
| topic | HepG2 Hepatocellular carcinoma Aloe vera Calligonum comosum Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2221169115303725 |
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