Automatic Synthesis and Micro PET/CT Imaging Study of 11C-DASB as a Serotonin Imaging Agent
Objectives: Automatic synthesis of 11C-DASB as a serotonin imaging agent was established and Micro PET/CT imaging with Wistar rat was performed. Methods: Optimization of 11C-DASB synthesis was established by modifying different 11C-methylation agents, solvent and reaction conditions. Automated proce...
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| Format: | Article |
| Language: | zho |
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Editorial Board of Journal of Isotopes
2015-01-01
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| Series: | Journal of Isotopes |
| Subjects: | |
| Online Access: | http://www.tws.org.cn/CN/10.7538/tws.2015.28.01.0001 |
| _version_ | 1818758145630011392 |
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| author | ZHANG Xiao-jun LI Yun-gang ZHANG Jin-ming |
| author_facet | ZHANG Xiao-jun LI Yun-gang ZHANG Jin-ming |
| author_sort | ZHANG Xiao-jun |
| collection | DOAJ |
| description | Objectives: Automatic synthesis of 11C-DASB as a serotonin imaging agent was established and Micro PET/CT imaging with Wistar rat was performed. Methods: Optimization of 11C-DASB synthesis was established by modifying different 11C-methylation agents, solvent and reaction conditions. Automated procedure for the synthesis of 11C-DASB was established, and Micro PET/CT imaging was complied with Wistar rat. Results: The optimized 11C-DASB synthesis was obtained. When 11C-CH3-Triflate was used as methylation agent, 500 μL DMSO was used to dissolve 1 mg precursor, the reaction was carried out at 80 ℃ for 2 min, the radiochemical synthesis yield was 63.7%. Micro PET/CT studies in rat showed that the initial uptake of 11C-DASB in the brain was high, especially in region of the highest density of SERT, such as colliculus and mid brain. Conclusion: The study should provide the basis for routine preparations of 11C-DASB with high reliability, it may be useful for PET imaging of SERT binding sites in the brain. |
| first_indexed | 2024-12-18T06:22:11Z |
| format | Article |
| id | doaj.art-25cfd4328b35400e9e9c9dd36f77ccc2 |
| institution | Directory Open Access Journal |
| issn | 1000-7512 1000-7512 |
| language | zho |
| last_indexed | 2024-12-18T06:22:11Z |
| publishDate | 2015-01-01 |
| publisher | Editorial Board of Journal of Isotopes |
| record_format | Article |
| series | Journal of Isotopes |
| spelling | doaj.art-25cfd4328b35400e9e9c9dd36f77ccc22022-12-21T21:18:08ZzhoEditorial Board of Journal of IsotopesJournal of Isotopes1000-75121000-75122015-01-012811610.7538/tws.2015.28.01.0001 Automatic Synthesis and Micro PET/CT Imaging Study of 11C-DASB as a Serotonin Imaging AgentZHANG Xiao-jun0LI Yun-gang1ZHANG Jin-mingLIU JianTIAN Jia-heObjectives: Automatic synthesis of 11C-DASB as a serotonin imaging agent was established and Micro PET/CT imaging with Wistar rat was performed. Methods: Optimization of 11C-DASB synthesis was established by modifying different 11C-methylation agents, solvent and reaction conditions. Automated procedure for the synthesis of 11C-DASB was established, and Micro PET/CT imaging was complied with Wistar rat. Results: The optimized 11C-DASB synthesis was obtained. When 11C-CH3-Triflate was used as methylation agent, 500 μL DMSO was used to dissolve 1 mg precursor, the reaction was carried out at 80 ℃ for 2 min, the radiochemical synthesis yield was 63.7%. Micro PET/CT studies in rat showed that the initial uptake of 11C-DASB in the brain was high, especially in region of the highest density of SERT, such as colliculus and mid brain. Conclusion: The study should provide the basis for routine preparations of 11C-DASB with high reliability, it may be useful for PET imaging of SERT binding sites in the brain.http://www.tws.org.cn/CN/10.7538/tws.2015.28.01.000111C-DASB SERT Micro PET/CT |
| spellingShingle | ZHANG Xiao-jun LI Yun-gang ZHANG Jin-ming Automatic Synthesis and Micro PET/CT Imaging Study of 11C-DASB as a Serotonin Imaging Agent Journal of Isotopes 11C-DASB SERT Micro PET/CT |
| title | Automatic Synthesis and Micro PET/CT Imaging Study of 11C-DASB as a Serotonin Imaging Agent |
| title_full | Automatic Synthesis and Micro PET/CT Imaging Study of 11C-DASB as a Serotonin Imaging Agent |
| title_fullStr | Automatic Synthesis and Micro PET/CT Imaging Study of 11C-DASB as a Serotonin Imaging Agent |
| title_full_unstemmed | Automatic Synthesis and Micro PET/CT Imaging Study of 11C-DASB as a Serotonin Imaging Agent |
| title_short | Automatic Synthesis and Micro PET/CT Imaging Study of 11C-DASB as a Serotonin Imaging Agent |
| title_sort | automatic synthesis and micro pet ct imaging study of 11c dasb as a serotonin imaging agent |
| topic | 11C-DASB SERT Micro PET/CT |
| url | http://www.tws.org.cn/CN/10.7538/tws.2015.28.01.0001 |
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