Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System
Chip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue for normal operation, this study presents a system model of the real-t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2010-01-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/10/1/697/ |
| _version_ | 1817991904798703616 |
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| author | Da-Sheng Lee |
| author_facet | Da-Sheng Lee |
| author_sort | Da-Sheng Lee |
| collection | DOAJ |
| description | Chip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue for normal operation, this study presents a system model of the real-time Polymerase Chain Reaction (PCR) machine to analyze the instrument design through numerical experiments. Based on model analysis, a systematic approach was developed to lower the variation of DNA quantification and achieve a robust design for a real-time PCR-on-a-chip system. Accelerated lift testing was adopted to evaluate the reliability of the chip prototype. According to the life test plan, this proposed real-time PCR-on-a-chip system was simulated to work continuously for over three years with similar reproducibility in DNA quantification. This not only shows the robustness of the lab-on-a-chip system, but also verifies the effectiveness of our systematic method for achieving a robust design. |
| first_indexed | 2024-04-14T01:19:29Z |
| format | Article |
| id | doaj.art-25d30a054c104d19b8f7eeb96cb50228 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T01:19:29Z |
| publishDate | 2010-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-25d30a054c104d19b8f7eeb96cb502282022-12-22T02:20:41ZengMDPI AGSensors1424-82202010-01-0110169771810.3390/s100100697Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip SystemDa-Sheng LeeChip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue for normal operation, this study presents a system model of the real-time Polymerase Chain Reaction (PCR) machine to analyze the instrument design through numerical experiments. Based on model analysis, a systematic approach was developed to lower the variation of DNA quantification and achieve a robust design for a real-time PCR-on-a-chip system. Accelerated lift testing was adopted to evaluate the reliability of the chip prototype. According to the life test plan, this proposed real-time PCR-on-a-chip system was simulated to work continuously for over three years with similar reproducibility in DNA quantification. This not only shows the robustness of the lab-on-a-chip system, but also verifies the effectiveness of our systematic method for achieving a robust design.http://www.mdpi.com/1424-8220/10/1/697/DNA quantification reliabilityrobust designsystem identification modelreal-time PCR machinereal-time PCR on-a-chip |
| spellingShingle | Da-Sheng Lee Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System Sensors DNA quantification reliability robust design system identification model real-time PCR machine real-time PCR on-a-chip |
| title | Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System |
| title_full | Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System |
| title_fullStr | Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System |
| title_full_unstemmed | Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System |
| title_short | Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System |
| title_sort | real time pcr machine system modeling and a systematic approach for the robust design of a real time pcr on a chip system |
| topic | DNA quantification reliability robust design system identification model real-time PCR machine real-time PCR on-a-chip |
| url | http://www.mdpi.com/1424-8220/10/1/697/ |
| work_keys_str_mv | AT dashenglee realtimepcrmachinesystemmodelingandasystematicapproachfortherobustdesignofarealtimepcronachipsystem |