Galleria mellonella: An Infection Model for Screening Compounds Against the Mycobacterium tuberculosis Complex
Drug screening models have a vital role in the development of novel antimycobacterial agents which are urgently needed to tackle drug-resistant tuberculosis (TB). We recently established the larvae of the insect Galleria mellonella (greater wax moth) as a novel infection model for the Mycobacterium...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02630/full |
| _version_ | 1817987742790844416 |
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| author | Masanori Asai Yanwen Li Jasmeet Singh Khara Jasmeet Singh Khara Brian D. Robertson Paul R. Langford Sandra M. Newton |
| author_facet | Masanori Asai Yanwen Li Jasmeet Singh Khara Jasmeet Singh Khara Brian D. Robertson Paul R. Langford Sandra M. Newton |
| author_sort | Masanori Asai |
| collection | DOAJ |
| description | Drug screening models have a vital role in the development of novel antimycobacterial agents which are urgently needed to tackle drug-resistant tuberculosis (TB). We recently established the larvae of the insect Galleria mellonella (greater wax moth) as a novel infection model for the Mycobacterium tuberculosis complex. Here we demonstrate its use as a rapid and reproducible screen to evaluate antimycobacterial drug efficacy using larvae infected with bioluminescent Mycobacterium bovis BCG lux. Treatment improved larval survival outcome and, with the exception of pyrazinamide, was associated with a significant reduction in in vivo mycobacterial bioluminescence over a 96 h period compared to the untreated controls. Isoniazid and rifampicin displayed the greatest in vivo efficacy and survival outcome. Thus G. mellonella, infected with bioluminescent mycobacteria, can rapidly determine in vivo drug efficacy, and has the potential to significantly reduce and/or replace the number of animals used in TB research. |
| first_indexed | 2024-04-14T00:25:23Z |
| format | Article |
| id | doaj.art-25d488c9df084770b2eda6894e8a791c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-14T00:25:23Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-25d488c9df084770b2eda6894e8a791c2022-12-22T02:22:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-11-011010.3389/fmicb.2019.02630487231Galleria mellonella: An Infection Model for Screening Compounds Against the Mycobacterium tuberculosis ComplexMasanori Asai0Yanwen Li1Jasmeet Singh Khara2Jasmeet Singh Khara3Brian D. Robertson4Paul R. Langford5Sandra M. Newton6Section of Paediatric Infectious Diseases, Department of Infectious Disease, Imperial College London, London, United KingdomDepartment of Pharmacy, National University of Singapore, Singapore, SingaporeSection of Paediatric Infectious Diseases, Department of Infectious Disease, Imperial College London, London, United KingdomDepartment of Pharmacy, National University of Singapore, Singapore, SingaporeMRC Centre for Molecular Bacteriology and Infection, Department of Infectious Disease, Imperial College London, London, United KingdomSection of Paediatric Infectious Diseases, Department of Infectious Disease, Imperial College London, London, United KingdomSection of Paediatric Infectious Diseases, Department of Infectious Disease, Imperial College London, London, United KingdomDrug screening models have a vital role in the development of novel antimycobacterial agents which are urgently needed to tackle drug-resistant tuberculosis (TB). We recently established the larvae of the insect Galleria mellonella (greater wax moth) as a novel infection model for the Mycobacterium tuberculosis complex. Here we demonstrate its use as a rapid and reproducible screen to evaluate antimycobacterial drug efficacy using larvae infected with bioluminescent Mycobacterium bovis BCG lux. Treatment improved larval survival outcome and, with the exception of pyrazinamide, was associated with a significant reduction in in vivo mycobacterial bioluminescence over a 96 h period compared to the untreated controls. Isoniazid and rifampicin displayed the greatest in vivo efficacy and survival outcome. Thus G. mellonella, infected with bioluminescent mycobacteria, can rapidly determine in vivo drug efficacy, and has the potential to significantly reduce and/or replace the number of animals used in TB research.https://www.frontiersin.org/article/10.3389/fmicb.2019.02630/fullGalleria mellonellatuberculosisinfection modelMycobacterium tuberculosis complexdrug screeningantimycobacterial agents |
| spellingShingle | Masanori Asai Yanwen Li Jasmeet Singh Khara Jasmeet Singh Khara Brian D. Robertson Paul R. Langford Sandra M. Newton Galleria mellonella: An Infection Model for Screening Compounds Against the Mycobacterium tuberculosis Complex Frontiers in Microbiology Galleria mellonella tuberculosis infection model Mycobacterium tuberculosis complex drug screening antimycobacterial agents |
| title | Galleria mellonella: An Infection Model for Screening Compounds Against the Mycobacterium tuberculosis Complex |
| title_full | Galleria mellonella: An Infection Model for Screening Compounds Against the Mycobacterium tuberculosis Complex |
| title_fullStr | Galleria mellonella: An Infection Model for Screening Compounds Against the Mycobacterium tuberculosis Complex |
| title_full_unstemmed | Galleria mellonella: An Infection Model for Screening Compounds Against the Mycobacterium tuberculosis Complex |
| title_short | Galleria mellonella: An Infection Model for Screening Compounds Against the Mycobacterium tuberculosis Complex |
| title_sort | galleria mellonella an infection model for screening compounds against the mycobacterium tuberculosis complex |
| topic | Galleria mellonella tuberculosis infection model Mycobacterium tuberculosis complex drug screening antimycobacterial agents |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02630/full |
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