Fission yeast arrestin-related trafficking adaptor, Arn1/Any1, is ubiquitinated by Pub1 E3 ligase and regulates endocytosis of Cat1 amino acid transporter
The Tsc1–Tsc2 complex homologous to human tuberous sclerosis complex proteins governs amino acid uptake by regulating the expression and intracellular distribution of amino acid transporters in Schizosaccharomyces pombe. Here, we performed a genetic screening for molecules that are involved in amino...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2014-05-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/3/6/542 |
| _version_ | 1818461277865902080 |
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| author | Akio Nakashima Shinji Kamada Fuyuhiko Tamanoi Ushio Kikkawa |
| author_facet | Akio Nakashima Shinji Kamada Fuyuhiko Tamanoi Ushio Kikkawa |
| author_sort | Akio Nakashima |
| collection | DOAJ |
| description | The Tsc1–Tsc2 complex homologous to human tuberous sclerosis complex proteins governs amino acid uptake by regulating the expression and intracellular distribution of amino acid transporters in Schizosaccharomyces pombe. Here, we performed a genetic screening for molecules that are involved in amino acid uptake and found Arn1 (also known as Any1). Arn1 is homologous to ART1, an arrestin-related trafficking adaptor (ART) in Saccharomyces cerevisiae, and contains a conserved arrestin motif, a ubiquitination site, and two PY motifs. Overexpression of arn1+ confers canavanine resistance on cells, whereas its disruption causes hypersensitivity to canavanine. We also show that Arn1 regulates endocytosis of the Cat1 amino acid transporter. Furthermore, deletion of arn1+ suppresses a defect of amino acid uptake and the aberrant Cat1 localization in tsc2Δ. Arn1 interacts with and is ubiquitinated by the Pub1 ubiquitin ligase, which is necessary to regulate Cat1 endocytosis. Cat1 undergoes ubiquitinations on lysine residues within the N-terminus, which are mediated, in part, by Arn1 to determine Cat1 localization. Correctively, Arn1 is an ART in S. pombe and contributes to amino acid uptake through regulating Cat1 endocytosis in which Tsc2 is involved. |
| first_indexed | 2024-12-14T23:43:36Z |
| format | Article |
| id | doaj.art-25d65b306dcc47bcbf7f1231c3b2dc30 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-14T23:43:36Z |
| publishDate | 2014-05-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-25d65b306dcc47bcbf7f1231c3b2dc302022-12-21T22:43:28ZengThe Company of BiologistsBiology Open2046-63902014-05-013654255210.1242/bio.2014836720148367Fission yeast arrestin-related trafficking adaptor, Arn1/Any1, is ubiquitinated by Pub1 E3 ligase and regulates endocytosis of Cat1 amino acid transporterAkio Nakashima0Shinji Kamada1Fuyuhiko Tamanoi2Ushio Kikkawa3 Biosignal Research Center, Kobe University, Kobe 657-8501, Japan Biosignal Research Center, Kobe University, Kobe 657-8501, Japan Department of Microbiology, Immunology and Molecular Genetics, Molecular Biology Institute, Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095-1489, USA Biosignal Research Center, Kobe University, Kobe 657-8501, Japan The Tsc1–Tsc2 complex homologous to human tuberous sclerosis complex proteins governs amino acid uptake by regulating the expression and intracellular distribution of amino acid transporters in Schizosaccharomyces pombe. Here, we performed a genetic screening for molecules that are involved in amino acid uptake and found Arn1 (also known as Any1). Arn1 is homologous to ART1, an arrestin-related trafficking adaptor (ART) in Saccharomyces cerevisiae, and contains a conserved arrestin motif, a ubiquitination site, and two PY motifs. Overexpression of arn1+ confers canavanine resistance on cells, whereas its disruption causes hypersensitivity to canavanine. We also show that Arn1 regulates endocytosis of the Cat1 amino acid transporter. Furthermore, deletion of arn1+ suppresses a defect of amino acid uptake and the aberrant Cat1 localization in tsc2Δ. Arn1 interacts with and is ubiquitinated by the Pub1 ubiquitin ligase, which is necessary to regulate Cat1 endocytosis. Cat1 undergoes ubiquitinations on lysine residues within the N-terminus, which are mediated, in part, by Arn1 to determine Cat1 localization. Correctively, Arn1 is an ART in S. pombe and contributes to amino acid uptake through regulating Cat1 endocytosis in which Tsc2 is involved.http://bio.biologists.org/content/3/6/542Arrestin-related trafficking adaptorARTArn1/Any1Amino acid uptakeEndocytosis of transporterUbiquitination |
| spellingShingle | Akio Nakashima Shinji Kamada Fuyuhiko Tamanoi Ushio Kikkawa Fission yeast arrestin-related trafficking adaptor, Arn1/Any1, is ubiquitinated by Pub1 E3 ligase and regulates endocytosis of Cat1 amino acid transporter Biology Open Arrestin-related trafficking adaptor ART Arn1/Any1 Amino acid uptake Endocytosis of transporter Ubiquitination |
| title | Fission yeast arrestin-related trafficking adaptor, Arn1/Any1, is ubiquitinated by Pub1 E3 ligase and regulates endocytosis of Cat1 amino acid transporter |
| title_full | Fission yeast arrestin-related trafficking adaptor, Arn1/Any1, is ubiquitinated by Pub1 E3 ligase and regulates endocytosis of Cat1 amino acid transporter |
| title_fullStr | Fission yeast arrestin-related trafficking adaptor, Arn1/Any1, is ubiquitinated by Pub1 E3 ligase and regulates endocytosis of Cat1 amino acid transporter |
| title_full_unstemmed | Fission yeast arrestin-related trafficking adaptor, Arn1/Any1, is ubiquitinated by Pub1 E3 ligase and regulates endocytosis of Cat1 amino acid transporter |
| title_short | Fission yeast arrestin-related trafficking adaptor, Arn1/Any1, is ubiquitinated by Pub1 E3 ligase and regulates endocytosis of Cat1 amino acid transporter |
| title_sort | fission yeast arrestin related trafficking adaptor arn1 any1 is ubiquitinated by pub1 e3 ligase and regulates endocytosis of cat1 amino acid transporter |
| topic | Arrestin-related trafficking adaptor ART Arn1/Any1 Amino acid uptake Endocytosis of transporter Ubiquitination |
| url | http://bio.biologists.org/content/3/6/542 |
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