LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis
Abstract Background Adventitious root (AR) formation is a complex genetic trait, which is controlled by various endogenous and environmental cues. Auxin is known to play a central role in AR formation; however, the mechanisms underlying this role are not well understood. Results In this study, we sh...
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1659-4 |
| _version_ | 1818148862454398976 |
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| author | Han Woo Lee Chuloh Cho Shashank K. Pandey Yoona Park Min-Jung Kim Jungmook Kim |
| author_facet | Han Woo Lee Chuloh Cho Shashank K. Pandey Yoona Park Min-Jung Kim Jungmook Kim |
| author_sort | Han Woo Lee |
| collection | DOAJ |
| description | Abstract Background Adventitious root (AR) formation is a complex genetic trait, which is controlled by various endogenous and environmental cues. Auxin is known to play a central role in AR formation; however, the mechanisms underlying this role are not well understood. Results In this study, we showed that a previously identified auxin signaling module, AUXIN RESPONSE FACTOR(ARF)7/ARF19-LATERAL ORGAN BOUNDARIES DOMAIN(LBD)16/LBD18 via AUXIN1(AUX1)/LIKE-AUXIN3 (LAX3) auxin influx carriers, which plays important roles in lateral root formation, is involved in AR formation in Arabidopsis. In aux1, lax3, arf7, arf19, lbd16 and lbd18 single mutants, we observed reduced numbers of ARs than in the wild type. Double and triple mutants exhibited an additional decrease in AR numbers compared with the corresponding single or double mutants, respectively, and the aux1 lax3 lbd16 lbd18 quadruple mutant was devoid of ARs. Expression of LBD16 or LBD18 under their own promoters in lbd16 or lbd18 mutants rescued the reduced number of ARs to wild-type levels. LBD16 or LBD18 fused to a dominant SRDX repressor suppressed promoter activity of the cell cycle gene, Cyclin-Dependent Kinase(CDK)A1;1, to some extent. Expression of LBD16 or LBD18 was significantly reduced in arf7 and arf19 mutants during AR formation in a light-dependent manner, but not in arf6 and arf8. GUS expression analysis of promoter-GUS reporter transgenic lines revealed overlapping expression patterns for LBD16, LBD18, ARF7, ARF19 and LAX3 in AR primordia. Conclusion These results suggest that the ARF7/ARF19-LBD16/LBD18 transcriptional module via the AUX1/LAX3 auxin influx carriers plays an important role in AR formation in Arabidopsis. |
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| format | Article |
| id | doaj.art-25d84425c0d84e2baf8816dd8669da10 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-11T12:57:53Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-25d84425c0d84e2baf8816dd8669da102022-12-22T01:06:31ZengBMCBMC Plant Biology1471-22292019-01-0119111110.1186/s12870-019-1659-4LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in ArabidopsisHan Woo Lee0Chuloh Cho1Shashank K. Pandey2Yoona Park3Min-Jung Kim4Jungmook Kim5Department of Bioenergy Science and Technology, Chonnam National UniversityDepartment of Bioenergy Science and Technology, Chonnam National UniversityDepartment of Bioenergy Science and Technology, Chonnam National UniversityDepartment of Bioenergy Science and Technology, Chonnam National UniversityDepartment of Bioenergy Science and Technology, Chonnam National UniversityDepartment of Bioenergy Science and Technology, Chonnam National UniversityAbstract Background Adventitious root (AR) formation is a complex genetic trait, which is controlled by various endogenous and environmental cues. Auxin is known to play a central role in AR formation; however, the mechanisms underlying this role are not well understood. Results In this study, we showed that a previously identified auxin signaling module, AUXIN RESPONSE FACTOR(ARF)7/ARF19-LATERAL ORGAN BOUNDARIES DOMAIN(LBD)16/LBD18 via AUXIN1(AUX1)/LIKE-AUXIN3 (LAX3) auxin influx carriers, which plays important roles in lateral root formation, is involved in AR formation in Arabidopsis. In aux1, lax3, arf7, arf19, lbd16 and lbd18 single mutants, we observed reduced numbers of ARs than in the wild type. Double and triple mutants exhibited an additional decrease in AR numbers compared with the corresponding single or double mutants, respectively, and the aux1 lax3 lbd16 lbd18 quadruple mutant was devoid of ARs. Expression of LBD16 or LBD18 under their own promoters in lbd16 or lbd18 mutants rescued the reduced number of ARs to wild-type levels. LBD16 or LBD18 fused to a dominant SRDX repressor suppressed promoter activity of the cell cycle gene, Cyclin-Dependent Kinase(CDK)A1;1, to some extent. Expression of LBD16 or LBD18 was significantly reduced in arf7 and arf19 mutants during AR formation in a light-dependent manner, but not in arf6 and arf8. GUS expression analysis of promoter-GUS reporter transgenic lines revealed overlapping expression patterns for LBD16, LBD18, ARF7, ARF19 and LAX3 in AR primordia. Conclusion These results suggest that the ARF7/ARF19-LBD16/LBD18 transcriptional module via the AUX1/LAX3 auxin influx carriers plays an important role in AR formation in Arabidopsis.http://link.springer.com/article/10.1186/s12870-019-1659-4Arabidopsis thalianaAdventitious root formationAuxin response factorLateral organ boundaries domainLBD16LBD18 |
| spellingShingle | Han Woo Lee Chuloh Cho Shashank K. Pandey Yoona Park Min-Jung Kim Jungmook Kim LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis BMC Plant Biology Arabidopsis thaliana Adventitious root formation Auxin response factor Lateral organ boundaries domain LBD16 LBD18 |
| title | LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis |
| title_full | LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis |
| title_fullStr | LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis |
| title_full_unstemmed | LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis |
| title_short | LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis |
| title_sort | lbd16 and lbd18 acting downstream of arf7 and arf19 are involved in adventitious root formation in arabidopsis |
| topic | Arabidopsis thaliana Adventitious root formation Auxin response factor Lateral organ boundaries domain LBD16 LBD18 |
| url | http://link.springer.com/article/10.1186/s12870-019-1659-4 |
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