Role of Serum Vitamin D, Interleukin 13, and microRNA-135a in Hepatocellular Carcinoma and Treatment Failure in Egyptian HCV-Infected Patients Receiving Direct Antiviral Agents
Direct-acting antivirals (DAAs) are used for hepatitis C virus (HCV) treatment. However, treatment failure and hepatocellular carcinoma (HCC) development following treatment was reported. In this study, we assessed the role of serum vitamin D, interleukin 13 (IL-13), and microRNA-135a in the predict...
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2021-10-01
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| Online Access: | https://www.mdpi.com/1999-4915/13/10/2008 |
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| author | Mohamed E. Ali Hamada M. Halby Mamdouh Yones Ali Elham Ahmed Hassan Mohamed A. El-Mokhtar Ibrahim M. Sayed Marwa M. Thabet Magdy Fouad Ahmed M. El-Ashmawy Zainab Gaber Mahran |
| author_facet | Mohamed E. Ali Hamada M. Halby Mamdouh Yones Ali Elham Ahmed Hassan Mohamed A. El-Mokhtar Ibrahim M. Sayed Marwa M. Thabet Magdy Fouad Ahmed M. El-Ashmawy Zainab Gaber Mahran |
| author_sort | Mohamed E. Ali |
| collection | DOAJ |
| description | Direct-acting antivirals (DAAs) are used for hepatitis C virus (HCV) treatment. However, treatment failure and hepatocellular carcinoma (HCC) development following treatment was reported. In this study, we assessed the role of serum vitamin D, interleukin 13 (IL-13), and microRNA-135a in the prediction of treatment failure with DAA and HCC development among Egyptian HCV-infected patients. A total of 950 patients with HCV-related chronic liver disease underwent DAA treatment. Before DAAs, serum vitamin D and IL-13 were determined by ELISA, and gene expression of miRNA-135a was assessed in serum by real-time PCR. The predictive abilities of these markers were determined using the receiver operating characteristic (ROC) curve. Sustained virological response (SVR) was achieved in 92.6% of HCV-infected patients (responders). High viral load, IL-13, miRNA-135a, and low vitamin D levels were associated with treatment failure and HCC development. HCC development was recorded in non-responders, but not in the responders (35.7% vs. 0% <i>p</i> < 0.001). In conclusion: serum IL-13, Vitamin D, and miRNA-135a could be potential biomarkers in monitoring DAA treatment and HCC prediction. DAAs-induced SVR may decrease the incidence of HCC. |
| first_indexed | 2024-03-10T06:08:14Z |
| format | Article |
| id | doaj.art-25da00ded3de4cd280c9df55322edea2 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T06:08:14Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-25da00ded3de4cd280c9df55322edea22023-11-22T20:19:22ZengMDPI AGViruses1999-49152021-10-011310200810.3390/v13102008Role of Serum Vitamin D, Interleukin 13, and microRNA-135a in Hepatocellular Carcinoma and Treatment Failure in Egyptian HCV-Infected Patients Receiving Direct Antiviral AgentsMohamed E. Ali0Hamada M. Halby1Mamdouh Yones Ali2Elham Ahmed Hassan3Mohamed A. El-Mokhtar4Ibrahim M. Sayed5Marwa M. Thabet6Magdy Fouad7Ahmed M. El-Ashmawy8Zainab Gaber Mahran9Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, EgyptDepartment of Gastroenterology and Tropical Medicine, Faculty of Medicine, Assiut University, Assiut 71515, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, EgyptDepartment of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut 71515, EgyptHepato-Gastroenterology Unit, Tropical Medicine Department, Faculty of Medicine, El-Minia University, Minya 61519, EgyptGastroenterology and Hepatology Unit, Department of Internal Medicine, Faculty of Medicine, Assiut University, Assiut 71515, EgyptDepartment of Gastroenterology and Tropical Medicine, Faculty of Medicine, Assiut University, Assiut 71515, EgyptDirect-acting antivirals (DAAs) are used for hepatitis C virus (HCV) treatment. However, treatment failure and hepatocellular carcinoma (HCC) development following treatment was reported. In this study, we assessed the role of serum vitamin D, interleukin 13 (IL-13), and microRNA-135a in the prediction of treatment failure with DAA and HCC development among Egyptian HCV-infected patients. A total of 950 patients with HCV-related chronic liver disease underwent DAA treatment. Before DAAs, serum vitamin D and IL-13 were determined by ELISA, and gene expression of miRNA-135a was assessed in serum by real-time PCR. The predictive abilities of these markers were determined using the receiver operating characteristic (ROC) curve. Sustained virological response (SVR) was achieved in 92.6% of HCV-infected patients (responders). High viral load, IL-13, miRNA-135a, and low vitamin D levels were associated with treatment failure and HCC development. HCC development was recorded in non-responders, but not in the responders (35.7% vs. 0% <i>p</i> < 0.001). In conclusion: serum IL-13, Vitamin D, and miRNA-135a could be potential biomarkers in monitoring DAA treatment and HCC prediction. DAAs-induced SVR may decrease the incidence of HCC.https://www.mdpi.com/1999-4915/13/10/2008HCVprognostic markerIL-13vit DmiRNA-135aHCC |
| spellingShingle | Mohamed E. Ali Hamada M. Halby Mamdouh Yones Ali Elham Ahmed Hassan Mohamed A. El-Mokhtar Ibrahim M. Sayed Marwa M. Thabet Magdy Fouad Ahmed M. El-Ashmawy Zainab Gaber Mahran Role of Serum Vitamin D, Interleukin 13, and microRNA-135a in Hepatocellular Carcinoma and Treatment Failure in Egyptian HCV-Infected Patients Receiving Direct Antiviral Agents Viruses HCV prognostic marker IL-13 vit D miRNA-135a HCC |
| title | Role of Serum Vitamin D, Interleukin 13, and microRNA-135a in Hepatocellular Carcinoma and Treatment Failure in Egyptian HCV-Infected Patients Receiving Direct Antiviral Agents |
| title_full | Role of Serum Vitamin D, Interleukin 13, and microRNA-135a in Hepatocellular Carcinoma and Treatment Failure in Egyptian HCV-Infected Patients Receiving Direct Antiviral Agents |
| title_fullStr | Role of Serum Vitamin D, Interleukin 13, and microRNA-135a in Hepatocellular Carcinoma and Treatment Failure in Egyptian HCV-Infected Patients Receiving Direct Antiviral Agents |
| title_full_unstemmed | Role of Serum Vitamin D, Interleukin 13, and microRNA-135a in Hepatocellular Carcinoma and Treatment Failure in Egyptian HCV-Infected Patients Receiving Direct Antiviral Agents |
| title_short | Role of Serum Vitamin D, Interleukin 13, and microRNA-135a in Hepatocellular Carcinoma and Treatment Failure in Egyptian HCV-Infected Patients Receiving Direct Antiviral Agents |
| title_sort | role of serum vitamin d interleukin 13 and microrna 135a in hepatocellular carcinoma and treatment failure in egyptian hcv infected patients receiving direct antiviral agents |
| topic | HCV prognostic marker IL-13 vit D miRNA-135a HCC |
| url | https://www.mdpi.com/1999-4915/13/10/2008 |
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