Integration of Transcriptome and Metabolome Analyses Reveals the Mechanistic Basis for Cadmium Accumulation in Maize
Summary: Cadmium (Cd) pollution in soil has become a major environmental issue worldwide. However, the underlying molecular mechanism of low grain-Cd accumulation (GCA) in maize is still largely unknown. Herein, we report the mechanistic basis for low GCA in maize by a multiomics approach. The low G...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222017564 |
| _version_ | 1811308548571791360 |
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| author | Kaina Lin Meng Zeng Darron V. Williams Weimin Hu Sergey Shabala Meixue Zhou Fangbin Cao |
| author_facet | Kaina Lin Meng Zeng Darron V. Williams Weimin Hu Sergey Shabala Meixue Zhou Fangbin Cao |
| author_sort | Kaina Lin |
| collection | DOAJ |
| description | Summary: Cadmium (Cd) pollution in soil has become a major environmental issue worldwide. However, the underlying molecular mechanism of low grain-Cd accumulation (GCA) in maize is still largely unknown. Herein, we report the mechanistic basis for low GCA in maize by a multiomics approach. The low GCA genotype L63 showed normal vacuolar formation and a lower capacity of xylem loading of Cd than the high-accumulator L42 under Cd stress. Transcriptomic sequencing identified 84 low-GCA-associated genes which are mainly involved in the S-adenosylmethionine (SAM) cycle, metal transport, and vacuolar sequestration. A metabolome analysis revealed that L63 plants had a more active SAM cycle and a greater capacity for terpenoid synthesis and phenylalanine metabolism than L42. Combining the analysis of transcriptome and metabolome characterized several genes as key genes involved in the determination of Cd accumulation. Our study identifies a mechanistic basis for low Cd accumulation in maize grains and provides candidate genes for genetic improvement of crops. |
| first_indexed | 2024-04-13T09:26:20Z |
| format | Article |
| id | doaj.art-25df32aacea546c0b051e564816728fa |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-13T09:26:20Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-25df32aacea546c0b051e564816728fa2022-12-22T02:52:26ZengElsevieriScience2589-00422022-12-012512105484Integration of Transcriptome and Metabolome Analyses Reveals the Mechanistic Basis for Cadmium Accumulation in MaizeKaina Lin0Meng Zeng1Darron V. Williams2Weimin Hu3Sergey Shabala4Meixue Zhou5Fangbin Cao6Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaDepartment of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaDepartment of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaDepartment of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaTasmanian Institute of Agriculture, University of Tasmania, Hobart, TAS, Australia; International Research Centre for Environmental Membrane Biology, Foshan University, Foshan 528000, ChinaTasmanian Institute of Agriculture, University of Tasmania, Hobart, TAS, Australia; Corresponding authorDepartment of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China; Corresponding authorSummary: Cadmium (Cd) pollution in soil has become a major environmental issue worldwide. However, the underlying molecular mechanism of low grain-Cd accumulation (GCA) in maize is still largely unknown. Herein, we report the mechanistic basis for low GCA in maize by a multiomics approach. The low GCA genotype L63 showed normal vacuolar formation and a lower capacity of xylem loading of Cd than the high-accumulator L42 under Cd stress. Transcriptomic sequencing identified 84 low-GCA-associated genes which are mainly involved in the S-adenosylmethionine (SAM) cycle, metal transport, and vacuolar sequestration. A metabolome analysis revealed that L63 plants had a more active SAM cycle and a greater capacity for terpenoid synthesis and phenylalanine metabolism than L42. Combining the analysis of transcriptome and metabolome characterized several genes as key genes involved in the determination of Cd accumulation. Our study identifies a mechanistic basis for low Cd accumulation in maize grains and provides candidate genes for genetic improvement of crops.http://www.sciencedirect.com/science/article/pii/S2589004222017564Plant biologyMetabolomicsTranscriptomics |
| spellingShingle | Kaina Lin Meng Zeng Darron V. Williams Weimin Hu Sergey Shabala Meixue Zhou Fangbin Cao Integration of Transcriptome and Metabolome Analyses Reveals the Mechanistic Basis for Cadmium Accumulation in Maize iScience Plant biology Metabolomics Transcriptomics |
| title | Integration of Transcriptome and Metabolome Analyses Reveals the Mechanistic Basis for Cadmium Accumulation in Maize |
| title_full | Integration of Transcriptome and Metabolome Analyses Reveals the Mechanistic Basis for Cadmium Accumulation in Maize |
| title_fullStr | Integration of Transcriptome and Metabolome Analyses Reveals the Mechanistic Basis for Cadmium Accumulation in Maize |
| title_full_unstemmed | Integration of Transcriptome and Metabolome Analyses Reveals the Mechanistic Basis for Cadmium Accumulation in Maize |
| title_short | Integration of Transcriptome and Metabolome Analyses Reveals the Mechanistic Basis for Cadmium Accumulation in Maize |
| title_sort | integration of transcriptome and metabolome analyses reveals the mechanistic basis for cadmium accumulation in maize |
| topic | Plant biology Metabolomics Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222017564 |
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