Methods of experiments planning by optimization of the nutrient medium for streptomycete
Nutritious medium for the streptomycete have been optimized by a method of experiment planning. Optimization of the fermentative medium by method combined planning of experiment (TFE 24 complete factorial) with a method of concentration gradient motion. Activity of staphylolytic enzymes synthesized...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Oles Honchar Dnipropetrovsk National University
2010-08-01
|
| Series: | Vìsnik Dnìpropetrovsʹkogo Unìversitetu: Serìâ Bìologìâ, Ekologìâ |
| Online Access: | http://ecology.dp.ua/index.php/ECO/article/view/102 |
| Summary: | Nutritious medium for the streptomycete have been optimized by a method of experiment planning. Optimization of the fermentative medium by method combined planning of experiment (TFE 24 complete factorial) with a method of concentration gradient motion. Activity of staphylolytic enzymes synthesized by Streptomyces recifensis var. lyticus 2Р-15 was studed as a parameter of the medium optimization. The enzymes activity averaged 5589.4 Un/ml in the medium optimized by a path-of-steepest-ascent method. That exceeds 69– 86 % up on the enzymes activity in a control medium derived by a simplex method of experiment planning. |
|---|---|
| ISSN: | 2310-0842 2312-301X |