Methods of experiments planning by optimization of the nutrient medium for streptomycete

Nutritious medium for the streptomycete have been optimized by a method of experiment planning. Optimization of the fermentative medium by method combined planning of experiment (TFE 24 complete factorial) with a method of concentration gradient motion. Activity of staphylolytic enzymes synthesized...

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Main Authors: I. V. Zhernosekova, N. P. Chernogor, А. А. Tymchuk, A. I. Vinnikov
Format: Article
Language:English
Published: Oles Honchar Dnipropetrovsk National University 2010-08-01
Series:Vìsnik Dnìpropetrovsʹkogo Unìversitetu: Serìâ Bìologìâ, Ekologìâ
Online Access:http://ecology.dp.ua/index.php/ECO/article/view/102
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author I. V. Zhernosekova
N. P. Chernogor
А. А. Tymchuk
A. I. Vinnikov
author_facet I. V. Zhernosekova
N. P. Chernogor
А. А. Tymchuk
A. I. Vinnikov
author_sort I. V. Zhernosekova
collection DOAJ
description Nutritious medium for the streptomycete have been optimized by a method of experiment planning. Optimization of the fermentative medium by method combined planning of experiment (TFE 24 complete factorial) with a method of concentration gradient motion. Activity of staphylolytic enzymes synthesized by Streptomyces recifensis var. lyticus 2Р-15 was studed as a parameter of the medium optimization. The enzymes activity averaged 5589.4 Un/ml in the medium optimized by a path-of-steepest-ascent method. That exceeds 69– 86 % up on the enzymes activity in a control medium derived by a simplex method of experiment planning.
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spelling doaj.art-25e29579b2f743a7bf24f2edea3ca1b62022-12-21T20:30:22ZengOles Honchar Dnipropetrovsk National UniversityVìsnik Dnìpropetrovsʹkogo Unìversitetu: Serìâ Bìologìâ, Ekologìâ2310-08422312-301X2010-08-01181202810.15421/011004102Methods of experiments planning by optimization of the nutrient medium for streptomyceteI. V. Zhernosekova0N. P. Chernogor1А. А. Tymchuk2A. I. Vinnikov3Днепропетровский национальный университет им. Олеся ГончараДнепропетровский национальный университет им. Олеся ГончараДнепропетровский национальный университет им. Олеся ГончараДнепропетровский национальный университет им. Олеся ГончараNutritious medium for the streptomycete have been optimized by a method of experiment planning. Optimization of the fermentative medium by method combined planning of experiment (TFE 24 complete factorial) with a method of concentration gradient motion. Activity of staphylolytic enzymes synthesized by Streptomyces recifensis var. lyticus 2Р-15 was studed as a parameter of the medium optimization. The enzymes activity averaged 5589.4 Un/ml in the medium optimized by a path-of-steepest-ascent method. That exceeds 69– 86 % up on the enzymes activity in a control medium derived by a simplex method of experiment planning.http://ecology.dp.ua/index.php/ECO/article/view/102
spellingShingle I. V. Zhernosekova
N. P. Chernogor
А. А. Tymchuk
A. I. Vinnikov
Methods of experiments planning by optimization of the nutrient medium for streptomycete
Vìsnik Dnìpropetrovsʹkogo Unìversitetu: Serìâ Bìologìâ, Ekologìâ
title Methods of experiments planning by optimization of the nutrient medium for streptomycete
title_full Methods of experiments planning by optimization of the nutrient medium for streptomycete
title_fullStr Methods of experiments planning by optimization of the nutrient medium for streptomycete
title_full_unstemmed Methods of experiments planning by optimization of the nutrient medium for streptomycete
title_short Methods of experiments planning by optimization of the nutrient medium for streptomycete
title_sort methods of experiments planning by optimization of the nutrient medium for streptomycete
url http://ecology.dp.ua/index.php/ECO/article/view/102
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