Methods of experiments planning by optimization of the nutrient medium for streptomycete
Nutritious medium for the streptomycete have been optimized by a method of experiment planning. Optimization of the fermentative medium by method combined planning of experiment (TFE 24 complete factorial) with a method of concentration gradient motion. Activity of staphylolytic enzymes synthesized...
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Format: | Article |
Language: | English |
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Oles Honchar Dnipropetrovsk National University
2010-08-01
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Series: | Vìsnik Dnìpropetrovsʹkogo Unìversitetu: Serìâ Bìologìâ, Ekologìâ |
Online Access: | http://ecology.dp.ua/index.php/ECO/article/view/102 |
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author | I. V. Zhernosekova N. P. Chernogor А. А. Tymchuk A. I. Vinnikov |
author_facet | I. V. Zhernosekova N. P. Chernogor А. А. Tymchuk A. I. Vinnikov |
author_sort | I. V. Zhernosekova |
collection | DOAJ |
description | Nutritious medium for the streptomycete have been optimized by a method of experiment planning. Optimization of the fermentative medium by method combined planning of experiment (TFE 24 complete factorial) with a method of concentration gradient motion. Activity of staphylolytic enzymes synthesized by Streptomyces recifensis var. lyticus 2Р-15 was studed as a parameter of the medium optimization. The enzymes activity averaged 5589.4 Un/ml in the medium optimized by a path-of-steepest-ascent method. That exceeds 69– 86 % up on the enzymes activity in a control medium derived by a simplex method of experiment planning. |
first_indexed | 2024-12-19T07:44:44Z |
format | Article |
id | doaj.art-25e29579b2f743a7bf24f2edea3ca1b6 |
institution | Directory Open Access Journal |
issn | 2310-0842 2312-301X |
language | English |
last_indexed | 2024-12-19T07:44:44Z |
publishDate | 2010-08-01 |
publisher | Oles Honchar Dnipropetrovsk National University |
record_format | Article |
series | Vìsnik Dnìpropetrovsʹkogo Unìversitetu: Serìâ Bìologìâ, Ekologìâ |
spelling | doaj.art-25e29579b2f743a7bf24f2edea3ca1b62022-12-21T20:30:22ZengOles Honchar Dnipropetrovsk National UniversityVìsnik Dnìpropetrovsʹkogo Unìversitetu: Serìâ Bìologìâ, Ekologìâ2310-08422312-301X2010-08-01181202810.15421/011004102Methods of experiments planning by optimization of the nutrient medium for streptomyceteI. V. Zhernosekova0N. P. Chernogor1А. А. Tymchuk2A. I. Vinnikov3Днепропетровский национальный университет им. Олеся ГончараДнепропетровский национальный университет им. Олеся ГончараДнепропетровский национальный университет им. Олеся ГончараДнепропетровский национальный университет им. Олеся ГончараNutritious medium for the streptomycete have been optimized by a method of experiment planning. Optimization of the fermentative medium by method combined planning of experiment (TFE 24 complete factorial) with a method of concentration gradient motion. Activity of staphylolytic enzymes synthesized by Streptomyces recifensis var. lyticus 2Р-15 was studed as a parameter of the medium optimization. The enzymes activity averaged 5589.4 Un/ml in the medium optimized by a path-of-steepest-ascent method. That exceeds 69– 86 % up on the enzymes activity in a control medium derived by a simplex method of experiment planning.http://ecology.dp.ua/index.php/ECO/article/view/102 |
spellingShingle | I. V. Zhernosekova N. P. Chernogor А. А. Tymchuk A. I. Vinnikov Methods of experiments planning by optimization of the nutrient medium for streptomycete Vìsnik Dnìpropetrovsʹkogo Unìversitetu: Serìâ Bìologìâ, Ekologìâ |
title | Methods of experiments planning by optimization of the nutrient medium for streptomycete |
title_full | Methods of experiments planning by optimization of the nutrient medium for streptomycete |
title_fullStr | Methods of experiments planning by optimization of the nutrient medium for streptomycete |
title_full_unstemmed | Methods of experiments planning by optimization of the nutrient medium for streptomycete |
title_short | Methods of experiments planning by optimization of the nutrient medium for streptomycete |
title_sort | methods of experiments planning by optimization of the nutrient medium for streptomycete |
url | http://ecology.dp.ua/index.php/ECO/article/view/102 |
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