Trans-Corneal Subretinal Injection in Mice and Its Effect on the Function and Morphology of the Retina.
To introduce a practical method of subretinal injection in mice and evaluate injection-induced retinal detachment (RD) and damage using a dynamic imaging system, electrophysiology, and histology.After full dilation of a 2-month-old C57BL/6J mouse pupil, the cornea near the limbus was punctured with...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4552822?pdf=render |
| _version_ | 1819240923309015040 |
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| author | Yan Qi Xufeng Dai Hua Zhang Ying He Yangyang Zhang Juanjuan Han Ping Zhu Yuxin Zhang Qinxiang Zheng Xia Li Chen Zhao Jijing Pang |
| author_facet | Yan Qi Xufeng Dai Hua Zhang Ying He Yangyang Zhang Juanjuan Han Ping Zhu Yuxin Zhang Qinxiang Zheng Xia Li Chen Zhao Jijing Pang |
| author_sort | Yan Qi |
| collection | DOAJ |
| description | To introduce a practical method of subretinal injection in mice and evaluate injection-induced retinal detachment (RD) and damage using a dynamic imaging system, electrophysiology, and histology.After full dilation of a 2-month-old C57BL/6J mouse pupil, the cornea near the limbus was punctured with a 30 ½-gague disposable beveled needle. A 33 ½-gauge blunt needle was inserted through the corneal perforation into the anterior chamber, avoiding the lens before going deeper into the vitreous cavity, and penetrating the inner retina to reach the subretinal space. The mice were divided into four groups: in group 1, about 80-100% of the retina was filled with subretinally injected solution; in group 2, approximately 50-70% of the retina was filled with injected solution; in group 3, the procedures were stopped before solution injection; and non-injected eyes were used as the negative control in group 4. An optical coherence tomography (OCT) imaging system was used to monitor retinal reattachment during the first three days following the injections. Histological and functional changes were examined by light microscopy and electroretinography (ERG) at five weeks post-injection.After a short-term training, a 70% success rate with 50% or more coverage (i.e., retinal blebs occupied 50% or more retinal area and filled with the injected solution) with minimal injection-related damages can be achieved. Bleb formation was associated with retinal detachment (RD) between the neuroretina and the retinal pigment epithelium (RPE) layer. Partial RD could be observed at post-injection day 1, and by day 2 most of the retina had reattached. At 5 weeks post-injection, compared to uninjected control group 4, the b-wave amplitudes of ERG decreased 22% in group 1, 16% in group 2, and 7% in group 3; the b-wave amplitudes were statistically different between the uninjected group and the groups with either 50-70% or 80-100% coverage. The subretinal injection-induced RD reattached and became stable at five weeks post-injection, although some photoreceptor damage could still be observed in and around the injection sites, especially in 80-100% coverage group.Trans-corneal subretinal injection is effective and practical, although subretinal injection-related damages can cause some morphological and functional loss. |
| first_indexed | 2024-12-23T14:15:43Z |
| format | Article |
| id | doaj.art-25e2c278396e4daf9431b9a9e7a369d2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T14:15:43Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-25e2c278396e4daf9431b9a9e7a369d22022-12-21T17:43:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013652310.1371/journal.pone.0136523Trans-Corneal Subretinal Injection in Mice and Its Effect on the Function and Morphology of the Retina.Yan QiXufeng DaiHua ZhangYing HeYangyang ZhangJuanjuan HanPing ZhuYuxin ZhangQinxiang ZhengXia LiChen ZhaoJijing PangTo introduce a practical method of subretinal injection in mice and evaluate injection-induced retinal detachment (RD) and damage using a dynamic imaging system, electrophysiology, and histology.After full dilation of a 2-month-old C57BL/6J mouse pupil, the cornea near the limbus was punctured with a 30 ½-gague disposable beveled needle. A 33 ½-gauge blunt needle was inserted through the corneal perforation into the anterior chamber, avoiding the lens before going deeper into the vitreous cavity, and penetrating the inner retina to reach the subretinal space. The mice were divided into four groups: in group 1, about 80-100% of the retina was filled with subretinally injected solution; in group 2, approximately 50-70% of the retina was filled with injected solution; in group 3, the procedures were stopped before solution injection; and non-injected eyes were used as the negative control in group 4. An optical coherence tomography (OCT) imaging system was used to monitor retinal reattachment during the first three days following the injections. Histological and functional changes were examined by light microscopy and electroretinography (ERG) at five weeks post-injection.After a short-term training, a 70% success rate with 50% or more coverage (i.e., retinal blebs occupied 50% or more retinal area and filled with the injected solution) with minimal injection-related damages can be achieved. Bleb formation was associated with retinal detachment (RD) between the neuroretina and the retinal pigment epithelium (RPE) layer. Partial RD could be observed at post-injection day 1, and by day 2 most of the retina had reattached. At 5 weeks post-injection, compared to uninjected control group 4, the b-wave amplitudes of ERG decreased 22% in group 1, 16% in group 2, and 7% in group 3; the b-wave amplitudes were statistically different between the uninjected group and the groups with either 50-70% or 80-100% coverage. The subretinal injection-induced RD reattached and became stable at five weeks post-injection, although some photoreceptor damage could still be observed in and around the injection sites, especially in 80-100% coverage group.Trans-corneal subretinal injection is effective and practical, although subretinal injection-related damages can cause some morphological and functional loss.http://europepmc.org/articles/PMC4552822?pdf=render |
| spellingShingle | Yan Qi Xufeng Dai Hua Zhang Ying He Yangyang Zhang Juanjuan Han Ping Zhu Yuxin Zhang Qinxiang Zheng Xia Li Chen Zhao Jijing Pang Trans-Corneal Subretinal Injection in Mice and Its Effect on the Function and Morphology of the Retina. PLoS ONE |
| title | Trans-Corneal Subretinal Injection in Mice and Its Effect on the Function and Morphology of the Retina. |
| title_full | Trans-Corneal Subretinal Injection in Mice and Its Effect on the Function and Morphology of the Retina. |
| title_fullStr | Trans-Corneal Subretinal Injection in Mice and Its Effect on the Function and Morphology of the Retina. |
| title_full_unstemmed | Trans-Corneal Subretinal Injection in Mice and Its Effect on the Function and Morphology of the Retina. |
| title_short | Trans-Corneal Subretinal Injection in Mice and Its Effect on the Function and Morphology of the Retina. |
| title_sort | trans corneal subretinal injection in mice and its effect on the function and morphology of the retina |
| url | http://europepmc.org/articles/PMC4552822?pdf=render |
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