Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation
Lateral opening of the LptDE transporter in the outer membrane of Gram-negative bacteria is necessary for insertion of lipopolysaccharides. Here, Botte et al. report a cryo-EM structure of a partially opened LptDE transporter, in complex with rigid chaperones derived from nanobodies.
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-29459-2 |
| _version_ | 1831724505507037184 |
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| author | Mathieu Botte Dongchun Ni Stephan Schenck Iwan Zimmermann Mohamed Chami Nicolas Bocquet Pascal Egloff Denis Bucher Matilde Trabuco Robert K. Y. Cheng Janine D. Brunner Markus A. Seeger Henning Stahlberg Michael Hennig |
| author_facet | Mathieu Botte Dongchun Ni Stephan Schenck Iwan Zimmermann Mohamed Chami Nicolas Bocquet Pascal Egloff Denis Bucher Matilde Trabuco Robert K. Y. Cheng Janine D. Brunner Markus A. Seeger Henning Stahlberg Michael Hennig |
| author_sort | Mathieu Botte |
| collection | DOAJ |
| description | Lateral opening of the LptDE transporter in the outer membrane of Gram-negative bacteria is necessary for insertion of lipopolysaccharides. Here, Botte et al. report a cryo-EM structure of a partially opened LptDE transporter, in complex with rigid chaperones derived from nanobodies. |
| first_indexed | 2024-12-21T04:22:52Z |
| format | Article |
| id | doaj.art-25e3d626c5e9434d8732b67049815a5b |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-21T04:22:52Z |
| publishDate | 2022-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-25e3d626c5e9434d8732b67049815a5b2022-12-21T19:16:07ZengNature PortfolioNature Communications2041-17232022-04-0113111010.1038/s41467-022-29459-2Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocationMathieu Botte0Dongchun Ni1Stephan Schenck2Iwan Zimmermann3Mohamed Chami4Nicolas Bocquet5Pascal Egloff6Denis Bucher7Matilde Trabuco8Robert K. Y. Cheng9Janine D. Brunner10Markus A. Seeger11Henning Stahlberg12Michael Hennig13leadXpro AG, Park InnovaareC-CINA, Biozentrum, University of BaselleadXpro AG, Park InnovaareInstitute of Medical Microbiology, University of ZürichC-CINA, Biozentrum, University of BaselleadXpro AG, Park InnovaareInstitute of Medical Microbiology, University of ZürichleadXpro AG, Park InnovaareleadXpro AG, Park InnovaareleadXpro AG, Park InnovaareLaboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institute (PSI)Institute of Medical Microbiology, University of ZürichC-CINA, Biozentrum, University of BaselleadXpro AG, Park InnovaareLateral opening of the LptDE transporter in the outer membrane of Gram-negative bacteria is necessary for insertion of lipopolysaccharides. Here, Botte et al. report a cryo-EM structure of a partially opened LptDE transporter, in complex with rigid chaperones derived from nanobodies.https://doi.org/10.1038/s41467-022-29459-2 |
| spellingShingle | Mathieu Botte Dongchun Ni Stephan Schenck Iwan Zimmermann Mohamed Chami Nicolas Bocquet Pascal Egloff Denis Bucher Matilde Trabuco Robert K. Y. Cheng Janine D. Brunner Markus A. Seeger Henning Stahlberg Michael Hennig Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation Nature Communications |
| title | Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation |
| title_full | Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation |
| title_fullStr | Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation |
| title_full_unstemmed | Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation |
| title_short | Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation |
| title_sort | cryo em structures of a lptde transporter in complex with pro macrobodies offer insight into lipopolysaccharide translocation |
| url | https://doi.org/10.1038/s41467-022-29459-2 |
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